Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet
Context Callistemon citrinus Skeels (Myrtaceae) exhibits many biological activities.Objective This study analyzes for the first time, the toxicity, obesogenic, and antioxidant effects of C. citrinus in rats fed with a high fat-fructose diet (HFFD).Materials and methods Four studies using male Wistar...
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Format: | Article |
Language: | English |
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Taylor & Francis Group
2022-12-01
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Series: | Pharmaceutical Biology |
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Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2022.2099907 |
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author | Luis Gerardo Ortega-Pérez Jonathan Saúl Piñón-Simental Oliver Rafid Magaña-Rodríguez Alejandro Lopéz-Mejía Luis Alberto Ayala-Ruiz Aram Josué García-Calderón Daniel Godínez-Hernández Patricia Rios-Chavez |
author_facet | Luis Gerardo Ortega-Pérez Jonathan Saúl Piñón-Simental Oliver Rafid Magaña-Rodríguez Alejandro Lopéz-Mejía Luis Alberto Ayala-Ruiz Aram Josué García-Calderón Daniel Godínez-Hernández Patricia Rios-Chavez |
author_sort | Luis Gerardo Ortega-Pérez |
collection | DOAJ |
description | Context Callistemon citrinus Skeels (Myrtaceae) exhibits many biological activities.Objective This study analyzes for the first time, the toxicity, obesogenic, and antioxidant effects of C. citrinus in rats fed with a high fat-fructose diet (HFFD).Materials and methods Four studies using male Wistar rats were conducted: (a) 7 groups (n = 3): control (corn oil) and ethanol extract of C. citrinus leaf (single oral dose at 100–4000 mg/kg) for acute toxicity; (b) 2 groups (n = 8): control (corn oil) and C. citrinus (1000 mg/kg/day) for 28 days for subacute toxicity; (c) 3 groups (n = 4) with single oral dose of lipid emulsion: control (lipid emulsion), C. citrinus and orlistat (250 and 50 mg/kg, respectively) for lipid absorption; (d) 4 groups (n = 6): control (normal diet) and 3 groups fed with HFFD: HFFD only, C. citrinus and simvastatin (oral dose 250 and 3 mg/kg, respectively) for 13 weeks. Antioxidant enzymes and biomarkers were evaluated and inhibition of pancreatic lipase was determined in vitro.Results Toxicological studies of C. citrinus showed no differences in biochemical parameters and lethal dose (LD50) was higher than 4000 mg/kg. C. citrinus inhibited pancreatic lipase activity, with IC50 of 392.00 µg/mL, and decreased lipid absorption by 70%. Additionally, it reduced the body weight 22%, restored the activities of antioxidant enzymes, and reduced the biomarkers of oxidative stress.Conclusions Callistemon citrinus showed an effect against oxidative stress by reducing biomarkers and induced antioxidant system, without toxic effects. |
first_indexed | 2024-04-11T21:41:46Z |
format | Article |
id | doaj.art-13e47aa3520846d4a3e9eccd7595b7ab |
institution | Directory Open Access Journal |
issn | 1388-0209 1744-5116 |
language | English |
last_indexed | 2024-04-11T21:41:46Z |
publishDate | 2022-12-01 |
publisher | Taylor & Francis Group |
record_format | Article |
series | Pharmaceutical Biology |
spelling | doaj.art-13e47aa3520846d4a3e9eccd7595b7ab2022-12-22T04:01:33ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162022-12-016011384139310.1080/13880209.2022.2099907Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose dietLuis Gerardo Ortega-Pérez0Jonathan Saúl Piñón-Simental1Oliver Rafid Magaña-Rodríguez2Alejandro Lopéz-Mejía3Luis Alberto Ayala-Ruiz4Aram Josué García-Calderón5Daniel Godínez-Hernández6Patricia Rios-Chavez7Facultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MéxicoFacultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MéxicoFacultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MéxicoFacultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MéxicoFacultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MéxicoFacultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MéxicoInstituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MéxicoFacultad de Biología, Universidad Michoacana de San Nicolás de Hidalgo, Morelia, Michoacán, MéxicoContext Callistemon citrinus Skeels (Myrtaceae) exhibits many biological activities.Objective This study analyzes for the first time, the toxicity, obesogenic, and antioxidant effects of C. citrinus in rats fed with a high fat-fructose diet (HFFD).Materials and methods Four studies using male Wistar rats were conducted: (a) 7 groups (n = 3): control (corn oil) and ethanol extract of C. citrinus leaf (single oral dose at 100–4000 mg/kg) for acute toxicity; (b) 2 groups (n = 8): control (corn oil) and C. citrinus (1000 mg/kg/day) for 28 days for subacute toxicity; (c) 3 groups (n = 4) with single oral dose of lipid emulsion: control (lipid emulsion), C. citrinus and orlistat (250 and 50 mg/kg, respectively) for lipid absorption; (d) 4 groups (n = 6): control (normal diet) and 3 groups fed with HFFD: HFFD only, C. citrinus and simvastatin (oral dose 250 and 3 mg/kg, respectively) for 13 weeks. Antioxidant enzymes and biomarkers were evaluated and inhibition of pancreatic lipase was determined in vitro.Results Toxicological studies of C. citrinus showed no differences in biochemical parameters and lethal dose (LD50) was higher than 4000 mg/kg. C. citrinus inhibited pancreatic lipase activity, with IC50 of 392.00 µg/mL, and decreased lipid absorption by 70%. Additionally, it reduced the body weight 22%, restored the activities of antioxidant enzymes, and reduced the biomarkers of oxidative stress.Conclusions Callistemon citrinus showed an effect against oxidative stress by reducing biomarkers and induced antioxidant system, without toxic effects.https://www.tandfonline.com/doi/10.1080/13880209.2022.2099907Alternative medicineoxidative stressoverweightliverkidneystomach |
spellingShingle | Luis Gerardo Ortega-Pérez Jonathan Saúl Piñón-Simental Oliver Rafid Magaña-Rodríguez Alejandro Lopéz-Mejía Luis Alberto Ayala-Ruiz Aram Josué García-Calderón Daniel Godínez-Hernández Patricia Rios-Chavez Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet Pharmaceutical Biology Alternative medicine oxidative stress overweight liver kidney stomach |
title | Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet |
title_full | Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet |
title_fullStr | Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet |
title_full_unstemmed | Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet |
title_short | Evaluation of the toxicology, anti-lipase, and antioxidant effects of Callistemon citrinus in rats fed with a high fat-fructose diet |
title_sort | evaluation of the toxicology anti lipase and antioxidant effects of callistemon citrinus in rats fed with a high fat fructose diet |
topic | Alternative medicine oxidative stress overweight liver kidney stomach |
url | https://www.tandfonline.com/doi/10.1080/13880209.2022.2099907 |
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