The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells.
Here, we report the results of a study on the effects of ethanol exposure on human red blood cells (RBCs) using quantitative phase imaging techniques at the level of individual cells. Three-dimensional refractive index tomograms and dynamic membrane fluctuations of RBCs were measured using common-pa...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145327&type=printable |
| _version_ | 1826584349835788288 |
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| author | Sang Yun Lee Hyun Joo Park Catherine Best-Popescu Seongsoo Jang Yong Keun Park |
| author_facet | Sang Yun Lee Hyun Joo Park Catherine Best-Popescu Seongsoo Jang Yong Keun Park |
| author_sort | Sang Yun Lee |
| collection | DOAJ |
| description | Here, we report the results of a study on the effects of ethanol exposure on human red blood cells (RBCs) using quantitative phase imaging techniques at the level of individual cells. Three-dimensional refractive index tomograms and dynamic membrane fluctuations of RBCs were measured using common-path diffraction optical tomography, from which morphological (volume, surface area, and sphericity); biochemical (hemoglobin (Hb) concentration and Hb content); and biomechanical (membrane fluctuation) parameters were retrieved at various concentrations of ethanol. RBCs exposed to the ethanol concentration of 0.1 and 0.3% v/v exhibited cell sphericities higher than those of normal cells. However, mean surface area and sphericity of RBCs in a lethal alcoholic condition (0.5% v/v) are not statistically different with those of healthy RBCs. Meanwhile, significant decreases of Hb content and concentration in RBC cytoplasm at the lethal condition were observed. Furthermore, dynamic fluctuation of RBC membranes increased significantly upon ethanol treatments, indicating ethanol-induced membrane fluidization. |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2025-03-14T15:36:21Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-13e7a15ceac447fdae330cbf51ec42552025-02-25T05:32:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011012e014532710.1371/journal.pone.0145327The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells.Sang Yun LeeHyun Joo ParkCatherine Best-PopescuSeongsoo JangYong Keun ParkHere, we report the results of a study on the effects of ethanol exposure on human red blood cells (RBCs) using quantitative phase imaging techniques at the level of individual cells. Three-dimensional refractive index tomograms and dynamic membrane fluctuations of RBCs were measured using common-path diffraction optical tomography, from which morphological (volume, surface area, and sphericity); biochemical (hemoglobin (Hb) concentration and Hb content); and biomechanical (membrane fluctuation) parameters were retrieved at various concentrations of ethanol. RBCs exposed to the ethanol concentration of 0.1 and 0.3% v/v exhibited cell sphericities higher than those of normal cells. However, mean surface area and sphericity of RBCs in a lethal alcoholic condition (0.5% v/v) are not statistically different with those of healthy RBCs. Meanwhile, significant decreases of Hb content and concentration in RBC cytoplasm at the lethal condition were observed. Furthermore, dynamic fluctuation of RBC membranes increased significantly upon ethanol treatments, indicating ethanol-induced membrane fluidization.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145327&type=printable |
| spellingShingle | Sang Yun Lee Hyun Joo Park Catherine Best-Popescu Seongsoo Jang Yong Keun Park The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells. PLoS ONE |
| title | The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells. |
| title_full | The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells. |
| title_fullStr | The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells. |
| title_full_unstemmed | The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells. |
| title_short | The Effects of Ethanol on the Morphological and Biochemical Properties of Individual Human Red Blood Cells. |
| title_sort | effects of ethanol on the morphological and biochemical properties of individual human red blood cells |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0145327&type=printable |
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