Gold Nanoparticles as a Direct and Rapid Sensor for Sensitive Analytical Detection of Biogenic Amines
Abstract A new optical sensor was developed for rapid screening with high sensitivity for the existence of biogenic amines (BAs) in poultry meat samples. Gold nanoparticles (GNPs) with particle size 11–19 nm function as a fast and sensitive biosensor for detection of histamine resulting from bacteri...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2017-03-01
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| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s11671-017-2014-z |
| _version_ | 1827839911438319616 |
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| author | K. M. A. El-Nour E. T. A. Salam H. M. Soliman A. S. Orabi |
| author_facet | K. M. A. El-Nour E. T. A. Salam H. M. Soliman A. S. Orabi |
| author_sort | K. M. A. El-Nour |
| collection | DOAJ |
| description | Abstract A new optical sensor was developed for rapid screening with high sensitivity for the existence of biogenic amines (BAs) in poultry meat samples. Gold nanoparticles (GNPs) with particle size 11–19 nm function as a fast and sensitive biosensor for detection of histamine resulting from bacterial decarboxylation of histidine as a spoilage marker for stored poultry meat. Upon reaction with histamine, the red color of the GNPs converted into deep blue. The appearance of blue color favorably coincides with the concentration of BAs that can induce symptoms of poisoning. This biosensor enables a semi-quantitative detection of analyte in real samples by eye-vision. Quality evaluation is carried out by measuring histamine and histidine using different analytical techniques such as UV–vis, FTIR, and fluorescence spectroscopy as well as TEM. A rapid quantitative readout of samples by UV–vis and fluorescence methods with standard instrumentation were proposed in a short time unlike chromatographic and electrophoretic methods. Sensitivity and limit of detection (LOD) of 6.59 × 10−4 and 0.6 μM, respectively, are determined for histamine as a spoilage marker with a correlation coefficient (R 2) of 0.993. |
| first_indexed | 2024-03-12T07:26:53Z |
| format | Article |
| id | doaj.art-13ebb5462571489680ad24a600aa95ac |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T07:26:53Z |
| publishDate | 2017-03-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-13ebb5462571489680ad24a600aa95ac2023-09-02T22:06:00ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2017-03-0112111110.1186/s11671-017-2014-zGold Nanoparticles as a Direct and Rapid Sensor for Sensitive Analytical Detection of Biogenic AminesK. M. A. El-Nour0E. T. A. Salam1H. M. Soliman2A. S. Orabi3Present Address: Department of Chemistry, College of Liberal Arts and Science, University of FloridaDepartment of Chemistry, Faculty of Science, Suez Canal UniversityDepartment of Chemistry, Faculty of Science, Suez Canal UniversityDepartment of Chemistry, Faculty of Science, Suez Canal UniversityAbstract A new optical sensor was developed for rapid screening with high sensitivity for the existence of biogenic amines (BAs) in poultry meat samples. Gold nanoparticles (GNPs) with particle size 11–19 nm function as a fast and sensitive biosensor for detection of histamine resulting from bacterial decarboxylation of histidine as a spoilage marker for stored poultry meat. Upon reaction with histamine, the red color of the GNPs converted into deep blue. The appearance of blue color favorably coincides with the concentration of BAs that can induce symptoms of poisoning. This biosensor enables a semi-quantitative detection of analyte in real samples by eye-vision. Quality evaluation is carried out by measuring histamine and histidine using different analytical techniques such as UV–vis, FTIR, and fluorescence spectroscopy as well as TEM. A rapid quantitative readout of samples by UV–vis and fluorescence methods with standard instrumentation were proposed in a short time unlike chromatographic and electrophoretic methods. Sensitivity and limit of detection (LOD) of 6.59 × 10−4 and 0.6 μM, respectively, are determined for histamine as a spoilage marker with a correlation coefficient (R 2) of 0.993.http://link.springer.com/article/10.1186/s11671-017-2014-zHistamineBiogenic aminesGold nanoparticlesSpoilage markerColorimetric sensor |
| spellingShingle | K. M. A. El-Nour E. T. A. Salam H. M. Soliman A. S. Orabi Gold Nanoparticles as a Direct and Rapid Sensor for Sensitive Analytical Detection of Biogenic Amines Nanoscale Research Letters Histamine Biogenic amines Gold nanoparticles Spoilage marker Colorimetric sensor |
| title | Gold Nanoparticles as a Direct and Rapid Sensor for Sensitive Analytical Detection of Biogenic Amines |
| title_full | Gold Nanoparticles as a Direct and Rapid Sensor for Sensitive Analytical Detection of Biogenic Amines |
| title_fullStr | Gold Nanoparticles as a Direct and Rapid Sensor for Sensitive Analytical Detection of Biogenic Amines |
| title_full_unstemmed | Gold Nanoparticles as a Direct and Rapid Sensor for Sensitive Analytical Detection of Biogenic Amines |
| title_short | Gold Nanoparticles as a Direct and Rapid Sensor for Sensitive Analytical Detection of Biogenic Amines |
| title_sort | gold nanoparticles as a direct and rapid sensor for sensitive analytical detection of biogenic amines |
| topic | Histamine Biogenic amines Gold nanoparticles Spoilage marker Colorimetric sensor |
| url | http://link.springer.com/article/10.1186/s11671-017-2014-z |
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