Mitochondrial genome data provide insights into the phylogenetic relationships within Triplophysa dalaica (Kessler, 1876) (Cypriniformes, Nemacheilidae)
Due to the detrimental effect of formaldehyde on DNA, ethanol has replaced formalin as the primary preservative for animal specimens. However, short-term formalin fixation of specimens might be applied during field collection. In an increasing number of studies, DNA extraction and sequencing have be...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2024-04-01
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| Series: | ZooKeys |
| Online Access: | https://zookeys.pensoft.net/article/116342/download/pdf/ |
| _version_ | 1797221535854886912 |
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| author | Hao Meng Yingnan Wang Ge-Xia Qiao Jun Chen |
| author_facet | Hao Meng Yingnan Wang Ge-Xia Qiao Jun Chen |
| author_sort | Hao Meng |
| collection | DOAJ |
| description | Due to the detrimental effect of formaldehyde on DNA, ethanol has replaced formalin as the primary preservative for animal specimens. However, short-term formalin fixation of specimens might be applied during field collection. In an increasing number of studies, DNA extraction and sequencing have been successfully conducted from formalin-fixed specimens. Here the DNA from five specimens of Triplophysa dalaica (Kessler, 1876) were extracted and performed high-throughput sequencing. Four of the specimens underwent short-term fixation with formalin and were subsequently transferred to ethanol. One was continuously stored in ethanol. No significant difference of DNA quality and amount were observed among these samples. Followed by assembly and annotation, five mitochondrial genomes ranging in length from 16,569 to 16,572 bp were obtained. Additionally, previously published data of other individuals or species were included to perform phylogenetic analyses. In the reconstructed trees, all eight individuals of T. dalaica form a monophyletic group within the Triplophysa branch. The group is divided into three clades: (1) samples from the Yellow River, (2) those from the Yangtze River, and (3) those from the Haihe River, and the Lake Dali Nur. This study sheds initial light on the phylogeographic relationships among different populations of T. dalaica, and will support the research about its evolutionary history in the future. |
| first_indexed | 2024-04-24T13:06:59Z |
| format | Article |
| id | doaj.art-13ec5df5b6ba4a879319c771bd92cba2 |
| institution | Directory Open Access Journal |
| issn | 1313-2970 |
| language | English |
| last_indexed | 2024-04-24T13:06:59Z |
| publishDate | 2024-04-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | ZooKeys |
| spelling | doaj.art-13ec5df5b6ba4a879319c771bd92cba22024-04-05T06:30:53ZengPensoft PublishersZooKeys1313-29702024-04-011197435510.3897/zookeys.1197.116342116342Mitochondrial genome data provide insights into the phylogenetic relationships within Triplophysa dalaica (Kessler, 1876) (Cypriniformes, Nemacheilidae)Hao Meng0Yingnan Wang1Ge-Xia Qiao2Jun Chen3Institute of Zoology, Chinese Academy of SciencesInstitute of Zoology, Chinese Academy of SciencesInstitute of Zoology, Chinese Academy of SciencesInstitute of Zoology, Chinese Academy of SciencesDue to the detrimental effect of formaldehyde on DNA, ethanol has replaced formalin as the primary preservative for animal specimens. However, short-term formalin fixation of specimens might be applied during field collection. In an increasing number of studies, DNA extraction and sequencing have been successfully conducted from formalin-fixed specimens. Here the DNA from five specimens of Triplophysa dalaica (Kessler, 1876) were extracted and performed high-throughput sequencing. Four of the specimens underwent short-term fixation with formalin and were subsequently transferred to ethanol. One was continuously stored in ethanol. No significant difference of DNA quality and amount were observed among these samples. Followed by assembly and annotation, five mitochondrial genomes ranging in length from 16,569 to 16,572 bp were obtained. Additionally, previously published data of other individuals or species were included to perform phylogenetic analyses. In the reconstructed trees, all eight individuals of T. dalaica form a monophyletic group within the Triplophysa branch. The group is divided into three clades: (1) samples from the Yellow River, (2) those from the Yangtze River, and (3) those from the Haihe River, and the Lake Dali Nur. This study sheds initial light on the phylogeographic relationships among different populations of T. dalaica, and will support the research about its evolutionary history in the future.https://zookeys.pensoft.net/article/116342/download/pdf/ |
| spellingShingle | Hao Meng Yingnan Wang Ge-Xia Qiao Jun Chen Mitochondrial genome data provide insights into the phylogenetic relationships within Triplophysa dalaica (Kessler, 1876) (Cypriniformes, Nemacheilidae) ZooKeys |
| title | Mitochondrial genome data provide insights into the phylogenetic relationships within Triplophysa dalaica (Kessler, 1876) (Cypriniformes, Nemacheilidae) |
| title_full | Mitochondrial genome data provide insights into the phylogenetic relationships within Triplophysa dalaica (Kessler, 1876) (Cypriniformes, Nemacheilidae) |
| title_fullStr | Mitochondrial genome data provide insights into the phylogenetic relationships within Triplophysa dalaica (Kessler, 1876) (Cypriniformes, Nemacheilidae) |
| title_full_unstemmed | Mitochondrial genome data provide insights into the phylogenetic relationships within Triplophysa dalaica (Kessler, 1876) (Cypriniformes, Nemacheilidae) |
| title_short | Mitochondrial genome data provide insights into the phylogenetic relationships within Triplophysa dalaica (Kessler, 1876) (Cypriniformes, Nemacheilidae) |
| title_sort | mitochondrial genome data provide insights into the phylogenetic relationships within triplophysa dalaica kessler 1876 cypriniformes nemacheilidae |
| url | https://zookeys.pensoft.net/article/116342/download/pdf/ |
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