The effect of a selective estrogen receptor modulator – raloxifene – on the levels of tryptophan and kynurenic acid in the livers of rats as studied via RP-HPLC-FL
The levels of tryptophan (TRP) and its main metabolite kynurenic acid (KYNA) were measured in rat livers treated with raloxifene – a selective estrogen receptor modulator. The research was conducted by applying high-performance liquid chromatography on a 5 μm Zorbax Eclipse XDB-C18 column. Selective...
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| Format: | Article |
| Language: | English |
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Sciendo
2020-09-01
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| Series: | Current Issues in Pharmacy and Medical Sciences |
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| Online Access: | https://doi.org/10.2478/cipms-2020-0029 |
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| author | Flieger Jolanta Pizon Magdalena Kowalska-Kepczynska Anna Smolinska Katarzyna Paluszkiewicz Piotr |
| author_facet | Flieger Jolanta Pizon Magdalena Kowalska-Kepczynska Anna Smolinska Katarzyna Paluszkiewicz Piotr |
| author_sort | Flieger Jolanta |
| collection | DOAJ |
| description | The levels of tryptophan (TRP) and its main metabolite kynurenic acid (KYNA) were measured in rat livers treated with raloxifene – a selective estrogen receptor modulator. The research was conducted by applying high-performance liquid chromatography on a 5 μm Zorbax Eclipse XDB-C18 column. Selective fluorescence detection (FL) was performed at an excitation of 219 nm and emission of 360 nm for TRP and KYNA. The assays showed good linearity (R2 >0.95) within the tested ranges of 0.045-0.20 µg mL−1, 0.025-0.32 µg mL−1, respectively, for KYNA and TRP. The limits of the detection were found to be 0.1480 ng mL−1 for KYNA and 0.0332 ng mL-1 for TRP. The deproteinization of the liver homogenate samples was accomplished by 80% methanol addition combined with boiling precipitation. The average recovery values were between 94.84% and 99.54% with RSDs no more than 5.5%. The work revealed that raloxifene decreased the mean value of tryptophan, as compared with the control group, while simultaneously leaving kynurenic acid at the same level. For the first time the research suggests that, in the case of raloxifene therapy, tryptophan is not metabolized via the kynurenine pathway. |
| first_indexed | 2024-12-17T06:44:28Z |
| format | Article |
| id | doaj.art-13ed05705e134d6c897af620b3582c50 |
| institution | Directory Open Access Journal |
| issn | 2084-980X 2300-6676 |
| language | English |
| last_indexed | 2024-12-17T06:44:28Z |
| publishDate | 2020-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Current Issues in Pharmacy and Medical Sciences |
| spelling | doaj.art-13ed05705e134d6c897af620b3582c502022-12-21T21:59:47ZengSciendoCurrent Issues in Pharmacy and Medical Sciences2084-980X2300-66762020-09-0133316216710.2478/cipms-2020-0029cipms-2020-0029The effect of a selective estrogen receptor modulator – raloxifene – on the levels of tryptophan and kynurenic acid in the livers of rats as studied via RP-HPLC-FLFlieger Jolanta0Pizon Magdalena1Kowalska-Kepczynska Anna2Smolinska Katarzyna3Paluszkiewicz Piotr4Department of Analytical Chemistry, Medical University of Lublin, PolandDepartment of Analytical Chemistry, Medical University of Lublin, PolandDepartment of Biochemical Diagnostics, Medical University of Lublin, PolandChair of Surgery and Surgical Nursing, Medical University of Lublin, PolandChair of Surgery and Surgical Nursing, Medical University of Lublin, PolandThe levels of tryptophan (TRP) and its main metabolite kynurenic acid (KYNA) were measured in rat livers treated with raloxifene – a selective estrogen receptor modulator. The research was conducted by applying high-performance liquid chromatography on a 5 μm Zorbax Eclipse XDB-C18 column. Selective fluorescence detection (FL) was performed at an excitation of 219 nm and emission of 360 nm for TRP and KYNA. The assays showed good linearity (R2 >0.95) within the tested ranges of 0.045-0.20 µg mL−1, 0.025-0.32 µg mL−1, respectively, for KYNA and TRP. The limits of the detection were found to be 0.1480 ng mL−1 for KYNA and 0.0332 ng mL-1 for TRP. The deproteinization of the liver homogenate samples was accomplished by 80% methanol addition combined with boiling precipitation. The average recovery values were between 94.84% and 99.54% with RSDs no more than 5.5%. The work revealed that raloxifene decreased the mean value of tryptophan, as compared with the control group, while simultaneously leaving kynurenic acid at the same level. For the first time the research suggests that, in the case of raloxifene therapy, tryptophan is not metabolized via the kynurenine pathway.https://doi.org/10.2478/cipms-2020-0029ryptophankynurenic acidliquid chromatographyraloxifene therapy |
| spellingShingle | Flieger Jolanta Pizon Magdalena Kowalska-Kepczynska Anna Smolinska Katarzyna Paluszkiewicz Piotr The effect of a selective estrogen receptor modulator – raloxifene – on the levels of tryptophan and kynurenic acid in the livers of rats as studied via RP-HPLC-FL Current Issues in Pharmacy and Medical Sciences ryptophan kynurenic acid liquid chromatography raloxifene therapy |
| title | The effect of a selective estrogen receptor modulator – raloxifene – on the levels of tryptophan and kynurenic acid in the livers of rats as studied via RP-HPLC-FL |
| title_full | The effect of a selective estrogen receptor modulator – raloxifene – on the levels of tryptophan and kynurenic acid in the livers of rats as studied via RP-HPLC-FL |
| title_fullStr | The effect of a selective estrogen receptor modulator – raloxifene – on the levels of tryptophan and kynurenic acid in the livers of rats as studied via RP-HPLC-FL |
| title_full_unstemmed | The effect of a selective estrogen receptor modulator – raloxifene – on the levels of tryptophan and kynurenic acid in the livers of rats as studied via RP-HPLC-FL |
| title_short | The effect of a selective estrogen receptor modulator – raloxifene – on the levels of tryptophan and kynurenic acid in the livers of rats as studied via RP-HPLC-FL |
| title_sort | effect of a selective estrogen receptor modulator raloxifene on the levels of tryptophan and kynurenic acid in the livers of rats as studied via rp hplc fl |
| topic | ryptophan kynurenic acid liquid chromatography raloxifene therapy |
| url | https://doi.org/10.2478/cipms-2020-0029 |
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