In-house reagent for creatinine in Synchron CX-3 delta
An in-house reagent for the determination of creatinine inserum was prepared and evaluated using the Beckman Synchron CX-3 delta automatic analyzer. The reagent was compared with the commercially available reagent. This study compared the in-house and the commercial reagent in 220 specimens. The acc...
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| Format: | Article |
| Language: | English |
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Prince of Songkla University
2008-01-01
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| Series: | Journal of Health Science and Medical Research (JHSMR) |
| Subjects: | |
| Online Access: | https://www.jhsmr.org/index.php/jhsmr/article/view/676 |
| _version_ | 1819077483260018688 |
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| author | P Choosongsang A Bodhikul P Ruengrairatanaroj B Wilirat P Choosongsang |
| author_facet | P Choosongsang A Bodhikul P Ruengrairatanaroj B Wilirat P Choosongsang |
| author_sort | P Choosongsang |
| collection | DOAJ |
| description | An in-house reagent for the determination of creatinine inserum was prepared and evaluated using the Beckman Synchron CX-3 delta automatic analyzer. The reagent was compared with the commercially available reagent. This study compared the in-house and the commercial reagent in 220 specimens. The accuracy assessed by the average recovery was 105.6% and 101.6% respectively. The precision was evaluated by analyzing three levels of the chemistry control serum. It was found that the within-run and between-run coefficients of variation (%CV) of the in-house reagent were 2.89% to 3.33% and 1.58% to 5.00%, whereas those of the commercial were 0.00% to 1.30% and 1.43% to 5.00% respectively. The inhouse and commercial reagent showed the same detection limit and linearity of 0.1 mg/dL and 25 mg/dL, respectively. The correlation (r) of creatinine was 0.997 (y=0.9919x+0.0557) with no significant difference found (p>0.05).
In conclusion, the in-house reagent showed satisfactory results for the determination of creatinine. It was found to be as good as the commercial reagent. Therefore, this in-house reagent for the determination of creatinine is applicable for routine use in clinical chemistry labs. Moreover, the cost of this reagent is about fifty times lower than that of the commercial reagent. |
| first_indexed | 2024-12-21T18:57:55Z |
| format | Article |
| id | doaj.art-13ee4246d1ac4002b983eecf5aa39f95 |
| institution | Directory Open Access Journal |
| issn | 2586-9981 2630-0559 |
| language | English |
| last_indexed | 2024-12-21T18:57:55Z |
| publishDate | 2008-01-01 |
| publisher | Prince of Songkla University |
| record_format | Article |
| series | Journal of Health Science and Medical Research (JHSMR) |
| spelling | doaj.art-13ee4246d1ac4002b983eecf5aa39f952022-12-21T18:53:33ZengPrince of Songkla UniversityJournal of Health Science and Medical Research (JHSMR)2586-99812630-05592008-01-012615360690In-house reagent for creatinine in Synchron CX-3 deltaP Choosongsang0A Bodhikul1P Ruengrairatanaroj2B Wilirat3P Choosongsang4Chemistry Unit, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110,Chemistry Unit, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110,Chemistry Unit, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110,Chemistry Unit, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110,Chemistry Unit, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla 90110,An in-house reagent for the determination of creatinine inserum was prepared and evaluated using the Beckman Synchron CX-3 delta automatic analyzer. The reagent was compared with the commercially available reagent. This study compared the in-house and the commercial reagent in 220 specimens. The accuracy assessed by the average recovery was 105.6% and 101.6% respectively. The precision was evaluated by analyzing three levels of the chemistry control serum. It was found that the within-run and between-run coefficients of variation (%CV) of the in-house reagent were 2.89% to 3.33% and 1.58% to 5.00%, whereas those of the commercial were 0.00% to 1.30% and 1.43% to 5.00% respectively. The inhouse and commercial reagent showed the same detection limit and linearity of 0.1 mg/dL and 25 mg/dL, respectively. The correlation (r) of creatinine was 0.997 (y=0.9919x+0.0557) with no significant difference found (p>0.05). In conclusion, the in-house reagent showed satisfactory results for the determination of creatinine. It was found to be as good as the commercial reagent. Therefore, this in-house reagent for the determination of creatinine is applicable for routine use in clinical chemistry labs. Moreover, the cost of this reagent is about fifty times lower than that of the commercial reagent.https://www.jhsmr.org/index.php/jhsmr/article/view/676automatic analyzercreatininein-house reagentcommercial reagent |
| spellingShingle | P Choosongsang A Bodhikul P Ruengrairatanaroj B Wilirat P Choosongsang In-house reagent for creatinine in Synchron CX-3 delta Journal of Health Science and Medical Research (JHSMR) automatic analyzer creatinine in-house reagent commercial reagent |
| title | In-house reagent for creatinine in Synchron CX-3 delta |
| title_full | In-house reagent for creatinine in Synchron CX-3 delta |
| title_fullStr | In-house reagent for creatinine in Synchron CX-3 delta |
| title_full_unstemmed | In-house reagent for creatinine in Synchron CX-3 delta |
| title_short | In-house reagent for creatinine in Synchron CX-3 delta |
| title_sort | in house reagent for creatinine in synchron cx 3 delta |
| topic | automatic analyzer creatinine in-house reagent commercial reagent |
| url | https://www.jhsmr.org/index.php/jhsmr/article/view/676 |
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