Confocal Endomicroscopy of Neuromuscular Junctions Stained with Physiologically Inert Protein Fragments of Tetanus Toxin

Confocal Endomicroscopy of Neuromuscular Junctions Stained with Physiologically Inert Protein Fragments of Tetanus Toxin

Live imaging of neuromuscular junctions (NMJs) in situ has been constrained by the suitability of ligands for inert vital staining of motor nerve terminals. Here, we constructed several truncated derivatives of the tetanus toxin C-fragment (TetC) fused with Emerald Fluorescent Protein (emGFP). Four...

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Bibliographic Details
Main Authors: Cornelia Roesl, Elizabeth R. Evans, Kosala N. Dissanayake, Veronika Boczonadi, Ross A. Jones, Graeme R. Jordan, Leire Ledahawsky, Guy C. C. Allen, Molly Scott, Alanna Thomson, Thomas M. Wishart, David I. Hughes, Richard J. Mead, Clifford C. Shone, Clarke R. Slater, Thomas H. Gillingwater, Paul A. Skehel, Richard R. Ribchester
Format: Article
Language:English
Published: MDPI AG 2021-10-01
Series:Biomolecules
Subjects:
neuromuscular junction
tetanus toxin
imaging
Online Access:https://www.mdpi.com/2218-273X/11/10/1499

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