Indirect Fluorescent Antibody Technique based Prevalence of Surra in Equines

This project was carried out to find the prevalence of trypanosomiasis in equine in District Gujranwala by using indirect fluorescent antibody technique and thin smear method. Blood samples were collected from a total of 200 horses and donkeys of different ages and either sex. Duplicate thin blood...

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Main Author: Ahsan Nadeem, Asim Aslam*, Zafar Iqbal Chaudhary, Kamran Ashraf1, Khalid Saeed1, Nisar Ahmad1, Ishtiaq Ahmed and Habib ur Rehman2
Format: Article
Language:English
Published: University of Agriculture, Faisalabad 2011-04-01
Series:Pakistan Veterinary Journal
Subjects:
Online Access:http://www.pvj.com.pk/pdf-files/31_2/169-170.pdf
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author Ahsan Nadeem, Asim Aslam*, Zafar Iqbal Chaudhary, Kamran Ashraf1, Khalid Saeed1, Nisar Ahmad1, Ishtiaq Ahmed and Habib ur Rehman2
author_facet Ahsan Nadeem, Asim Aslam*, Zafar Iqbal Chaudhary, Kamran Ashraf1, Khalid Saeed1, Nisar Ahmad1, Ishtiaq Ahmed and Habib ur Rehman2
author_sort Ahsan Nadeem, Asim Aslam*, Zafar Iqbal Chaudhary, Kamran Ashraf1, Khalid Saeed1, Nisar Ahmad1, Ishtiaq Ahmed and Habib ur Rehman2
collection DOAJ
description This project was carried out to find the prevalence of trypanosomiasis in equine in District Gujranwala by using indirect fluorescent antibody technique and thin smear method. Blood samples were collected from a total of 200 horses and donkeys of different ages and either sex. Duplicate thin blood smears were prepared from each sample and remaining blood samples were centrifuged to separate the serum. Smears from each animal were processed for giemsa staining and indirect fluorescent antibody test (IFAT). Giemsa stained smears revealed Trypanosome infection in 4/200 (2.0%) samples and IFAT in 12/200 (6.0%) animals.
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spelling doaj.art-13ef85737c95423f8f866c4e52f1562d2022-12-22T02:52:24ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182011-04-01312169170Indirect Fluorescent Antibody Technique based Prevalence of Surra in EquinesAhsan Nadeem, Asim Aslam*, Zafar Iqbal Chaudhary, Kamran Ashraf1, Khalid Saeed1, Nisar Ahmad1, Ishtiaq Ahmed and Habib ur Rehman2This project was carried out to find the prevalence of trypanosomiasis in equine in District Gujranwala by using indirect fluorescent antibody technique and thin smear method. Blood samples were collected from a total of 200 horses and donkeys of different ages and either sex. Duplicate thin blood smears were prepared from each sample and remaining blood samples were centrifuged to separate the serum. Smears from each animal were processed for giemsa staining and indirect fluorescent antibody test (IFAT). Giemsa stained smears revealed Trypanosome infection in 4/200 (2.0%) samples and IFAT in 12/200 (6.0%) animals.http://www.pvj.com.pk/pdf-files/31_2/169-170.pdfEquinesIndirect fluorescentantibody techniqueTrypanosomiasis
spellingShingle Ahsan Nadeem, Asim Aslam*, Zafar Iqbal Chaudhary, Kamran Ashraf1, Khalid Saeed1, Nisar Ahmad1, Ishtiaq Ahmed and Habib ur Rehman2
Indirect Fluorescent Antibody Technique based Prevalence of Surra in Equines
Pakistan Veterinary Journal
Equines
Indirect fluorescent
antibody technique
Trypanosomiasis
title Indirect Fluorescent Antibody Technique based Prevalence of Surra in Equines
title_full Indirect Fluorescent Antibody Technique based Prevalence of Surra in Equines
title_fullStr Indirect Fluorescent Antibody Technique based Prevalence of Surra in Equines
title_full_unstemmed Indirect Fluorescent Antibody Technique based Prevalence of Surra in Equines
title_short Indirect Fluorescent Antibody Technique based Prevalence of Surra in Equines
title_sort indirect fluorescent antibody technique based prevalence of surra in equines
topic Equines
Indirect fluorescent
antibody technique
Trypanosomiasis
url http://www.pvj.com.pk/pdf-files/31_2/169-170.pdf
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