Brucellosis in wild boar (Sus scrofa) in the Republic of Croati
During the years 2001 and 2002 on seven localities in Croatiaa survey on the prevalence of brucellosis in wild boar was carried out. The survey included 271 (52.7%) female and 243 (47.3%) male animals between 7 months and 4 years of age and weighing from 14 to 135 kg. On that occasion 514 blood samp...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2004-04-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200404-0003_brucellosis-in-wild-boar-sus-scrofa-in-the-republic-of-croati.php |
| _version_ | 1828006903583604736 |
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| author | Z. Cvetnic J. Toncic S. Spicic M. Lojkic S. Terzic L. Jemersic A. Humski S. Curic M. Mitak B. Habrun M. Brstilo M. Ocepek B. Krt |
| author_facet | Z. Cvetnic J. Toncic S. Spicic M. Lojkic S. Terzic L. Jemersic A. Humski S. Curic M. Mitak B. Habrun M. Brstilo M. Ocepek B. Krt |
| author_sort | Z. Cvetnic |
| collection | DOAJ |
| description | During the years 2001 and 2002 on seven localities in Croatiaa survey on the prevalence of brucellosis in wild boar was carried out. The survey included 271 (52.7%) female and 243 (47.3%) male animals between 7 months and 4 years of age and weighing from 14 to 135 kg. On that occasion 514 blood samples of wild boar were serologically analysed. For serological analysis indirect enzyme immunoassay (iELISA), Rose Bengal test (RBT), complement fixation test (CFT) and slow agglutination test (SAT) were used. In all of the wild boar from all of the localities investigated positive reactions to brucellosis were established. Most of the positive reactions were established by iELISA (13.6%), then by RBT (11.5%), CFT (10.5%) and SAT (8.9%). Tissue samples of 106 animals: testes samples from 67 animals, uterus tissue from 38 animals and 5 fetuses of piglets from 1 mother were analysed bacteriologically. Brucella suis biovar 2 was isolated from 18 (17.0%) animals that originated from all of the localities investigated. Isolates were identified by PCR using BRU-UP and BRU-LOW primers specific for genus Brucella and primers specific for IS711. Based on our results it could be concluded that in Croatia wild boar are natural vector and/or reservoirs of B. suis biovar 2. This permanent risk factor is hazardious for domestic and wild animals in theRepublic ofCroatia. |
| first_indexed | 2024-04-10T08:02:36Z |
| format | Article |
| id | doaj.art-13f0adb07150440f96ab9772646da339 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:02:36Z |
| publishDate | 2004-04-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-13f0adb07150440f96ab9772646da3392023-02-23T03:49:09ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922004-04-0149411512210.17221/5684-VETMEDvet-200404-0003Brucellosis in wild boar (Sus scrofa) in the Republic of CroatiZ. Cvetnic0J. Toncic1S. Spicic2M. Lojkic3S. Terzic4L. Jemersic5A. Humski6S. Curic7M. Mitak8B. Habrun9M. Brstilo10M. Ocepek11B. Krt12Croatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaCroatian Veterinary Institute, Zagreb, CroatiaDuring the years 2001 and 2002 on seven localities in Croatiaa survey on the prevalence of brucellosis in wild boar was carried out. The survey included 271 (52.7%) female and 243 (47.3%) male animals between 7 months and 4 years of age and weighing from 14 to 135 kg. On that occasion 514 blood samples of wild boar were serologically analysed. For serological analysis indirect enzyme immunoassay (iELISA), Rose Bengal test (RBT), complement fixation test (CFT) and slow agglutination test (SAT) were used. In all of the wild boar from all of the localities investigated positive reactions to brucellosis were established. Most of the positive reactions were established by iELISA (13.6%), then by RBT (11.5%), CFT (10.5%) and SAT (8.9%). Tissue samples of 106 animals: testes samples from 67 animals, uterus tissue from 38 animals and 5 fetuses of piglets from 1 mother were analysed bacteriologically. Brucella suis biovar 2 was isolated from 18 (17.0%) animals that originated from all of the localities investigated. Isolates were identified by PCR using BRU-UP and BRU-LOW primers specific for genus Brucella and primers specific for IS711. Based on our results it could be concluded that in Croatia wild boar are natural vector and/or reservoirs of B. suis biovar 2. This permanent risk factor is hazardious for domestic and wild animals in theRepublic ofCroatia.https://vetmed.agriculturejournals.cz/artkey/vet-200404-0003_brucellosis-in-wild-boar-sus-scrofa-in-the-republic-of-croati.phpzoonosesrisk assessmentpig industrywild boarbrucella suis biovar 2 |
| spellingShingle | Z. Cvetnic J. Toncic S. Spicic M. Lojkic S. Terzic L. Jemersic A. Humski S. Curic M. Mitak B. Habrun M. Brstilo M. Ocepek B. Krt Brucellosis in wild boar (Sus scrofa) in the Republic of Croati Veterinární Medicína zoonoses risk assessment pig industry wild boar brucella suis biovar 2 |
| title | Brucellosis in wild boar (Sus scrofa) in the Republic of Croati |
| title_full | Brucellosis in wild boar (Sus scrofa) in the Republic of Croati |
| title_fullStr | Brucellosis in wild boar (Sus scrofa) in the Republic of Croati |
| title_full_unstemmed | Brucellosis in wild boar (Sus scrofa) in the Republic of Croati |
| title_short | Brucellosis in wild boar (Sus scrofa) in the Republic of Croati |
| title_sort | brucellosis in wild boar sus scrofa in the republic of croati |
| topic | zoonoses risk assessment pig industry wild boar brucella suis biovar 2 |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200404-0003_brucellosis-in-wild-boar-sus-scrofa-in-the-republic-of-croati.php |
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