Safranal, an active constituent of saffron, ameliorates myocardial ischemia via reduction of oxidative stress and regulation of Ca2+ homeostasis
Safranal (SFR) is the major constituent of saffron. The purpose of this study was to observe the effect of SFR on myocardial ischemia induced by isoprenaline (ISO) and to explore its possible mechanism. The myocardial ischemia rat model was established by subcutaneous injection of ISO (85 mg/kg/d) o...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-07-01
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| Series: | Journal of Pharmacological Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861320300323 |
| _version_ | 1817986949119475712 |
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| author | Yurun Xue Weiyue Jin Yucong Xue Yuanyuan Zhang Hongfang Wang Yixin Zhang Shengjiang Guan Xi Chu Jianping Zhang |
| author_facet | Yurun Xue Weiyue Jin Yucong Xue Yuanyuan Zhang Hongfang Wang Yixin Zhang Shengjiang Guan Xi Chu Jianping Zhang |
| author_sort | Yurun Xue |
| collection | DOAJ |
| description | Safranal (SFR) is the major constituent of saffron. The purpose of this study was to observe the effect of SFR on myocardial ischemia induced by isoprenaline (ISO) and to explore its possible mechanism. The myocardial ischemia rat model was established by subcutaneous injection of ISO (85 mg/kg/d) on the 8th and 9th day of the experiment. Serum creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA) and superoxide dismutase (SOD) were measured, as were changes in calcium concentration, reactive oxygen species (ROS) and cardiac morphology of the myocardial tissue. The effects of SFR on cell contraction, Ca2+ transient and L-type Ca2+ current (ICa-L) in isolated rat myocardial cells were measured using the Ion Optix detection system and the whole-cell patch-clamp technique. SFR can decrease the activity of serum CK, LDH and MDA, and increase the activity of serum SOD, reduce intracellular calcium concentration and the manufacture of ROS. In addition, SFR can improve changes in heart morphology. SFR can significantly inhibit contraction, Ca2+ transients and ICa-L in isolated ventricular myocytes. SFR has a cardioprotective role in ISO-induced MI rats, and the underling mechanism is related to the inhibition of oxidative stress, myocardial contractility, ICa-L and the regulation of Ca2+ homeostasis. |
| first_indexed | 2024-04-14T00:15:48Z |
| format | Article |
| id | doaj.art-13f0ba544d324d56a0772d38add965b0 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-04-14T00:15:48Z |
| publishDate | 2020-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-13f0ba544d324d56a0772d38add965b02022-12-22T02:23:08ZengElsevierJournal of Pharmacological Sciences1347-86132020-07-011433156164Safranal, an active constituent of saffron, ameliorates myocardial ischemia via reduction of oxidative stress and regulation of Ca2+ homeostasisYurun Xue0Weiyue Jin1Yucong Xue2Yuanyuan Zhang3Hongfang Wang4Yixin Zhang5Shengjiang Guan6Xi Chu7Jianping Zhang8School of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, ChinaSchool of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, ChinaSchool of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, ChinaSchool of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, ChinaSchool of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, ChinaSchool of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, ChinaSchool of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China; Affiliated Hospital, Hebei University of Chinese Medicine, Shijiazhuang 050011, Hebei, China; Corresponding author. School of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China. Fax: +86 311 89926719.Department of Pharmacy, The Fourth Hospital of Hebei Medical University, No.12, Jiankang Road, Shijiazhuang 050011, Hebei, China; Corresponding author.School of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China; Hebei Key Laboratory of Chinese Medicine Research on Cardio-cerebrovascular Disease, Shijiazhuang 050200, Hebei, China; Corresponding author. School of Basic Medicine, Hebei University of Chinese Medicine, Shijiazhuang 050200, Hebei, China. Fax: +86 311 89926719.Safranal (SFR) is the major constituent of saffron. The purpose of this study was to observe the effect of SFR on myocardial ischemia induced by isoprenaline (ISO) and to explore its possible mechanism. The myocardial ischemia rat model was established by subcutaneous injection of ISO (85 mg/kg/d) on the 8th and 9th day of the experiment. Serum creatine kinase (CK), lactate dehydrogenase (LDH), malondialdehyde (MDA) and superoxide dismutase (SOD) were measured, as were changes in calcium concentration, reactive oxygen species (ROS) and cardiac morphology of the myocardial tissue. The effects of SFR on cell contraction, Ca2+ transient and L-type Ca2+ current (ICa-L) in isolated rat myocardial cells were measured using the Ion Optix detection system and the whole-cell patch-clamp technique. SFR can decrease the activity of serum CK, LDH and MDA, and increase the activity of serum SOD, reduce intracellular calcium concentration and the manufacture of ROS. In addition, SFR can improve changes in heart morphology. SFR can significantly inhibit contraction, Ca2+ transients and ICa-L in isolated ventricular myocytes. SFR has a cardioprotective role in ISO-induced MI rats, and the underling mechanism is related to the inhibition of oxidative stress, myocardial contractility, ICa-L and the regulation of Ca2+ homeostasis.http://www.sciencedirect.com/science/article/pii/S1347861320300323SafranalMyocardial ischemiaOxidative stressCell contractilityL-type Ca2+ current |
| spellingShingle | Yurun Xue Weiyue Jin Yucong Xue Yuanyuan Zhang Hongfang Wang Yixin Zhang Shengjiang Guan Xi Chu Jianping Zhang Safranal, an active constituent of saffron, ameliorates myocardial ischemia via reduction of oxidative stress and regulation of Ca2+ homeostasis Journal of Pharmacological Sciences Safranal Myocardial ischemia Oxidative stress Cell contractility L-type Ca2+ current |
| title | Safranal, an active constituent of saffron, ameliorates myocardial ischemia via reduction of oxidative stress and regulation of Ca2+ homeostasis |
| title_full | Safranal, an active constituent of saffron, ameliorates myocardial ischemia via reduction of oxidative stress and regulation of Ca2+ homeostasis |
| title_fullStr | Safranal, an active constituent of saffron, ameliorates myocardial ischemia via reduction of oxidative stress and regulation of Ca2+ homeostasis |
| title_full_unstemmed | Safranal, an active constituent of saffron, ameliorates myocardial ischemia via reduction of oxidative stress and regulation of Ca2+ homeostasis |
| title_short | Safranal, an active constituent of saffron, ameliorates myocardial ischemia via reduction of oxidative stress and regulation of Ca2+ homeostasis |
| title_sort | safranal an active constituent of saffron ameliorates myocardial ischemia via reduction of oxidative stress and regulation of ca2 homeostasis |
| topic | Safranal Myocardial ischemia Oxidative stress Cell contractility L-type Ca2+ current |
| url | http://www.sciencedirect.com/science/article/pii/S1347861320300323 |
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