Molecular Characteristics of Colistin Resistance in <i>Acinetobacter baumannii</i> and the Activity of Antimicrobial Combination Therapy in a Tertiary Care Medical Center in Lebanon
(1) Background: Infections with pan-drug-resistant (PDR) bacteria, such as <i>A. baumannii</i>, are becoming increasingly common, especially in healthcare facilities. In this study, we selected 15 colistin-resistant clinical <i>A. baumannii</i> isolates from a hospital in Bei...
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| Format: | Article |
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MDPI AG
2024-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/12/2/349 |
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| author | Antoine Abou Fayad Louis-Patrick Haraoui Ahmad Sleiman Hadi Hussein Frédéric Grenier Ghada Derbaj Dana Itani Sereen Iweir Nour Sherri Wael Bazzi Sari Rasheed Arax Tanelian Mariam Miari Bassam el Hafi Souha S. Kanj Zeina A. Kanafani Ziad Daoud George F. Araj Ghassan M. Matar |
| author_facet | Antoine Abou Fayad Louis-Patrick Haraoui Ahmad Sleiman Hadi Hussein Frédéric Grenier Ghada Derbaj Dana Itani Sereen Iweir Nour Sherri Wael Bazzi Sari Rasheed Arax Tanelian Mariam Miari Bassam el Hafi Souha S. Kanj Zeina A. Kanafani Ziad Daoud George F. Araj Ghassan M. Matar |
| author_sort | Antoine Abou Fayad |
| collection | DOAJ |
| description | (1) Background: Infections with pan-drug-resistant (PDR) bacteria, such as <i>A. baumannii</i>, are becoming increasingly common, especially in healthcare facilities. In this study, we selected 15 colistin-resistant clinical <i>A. baumannii</i> isolates from a hospital in Beirut, Lebanon, to test combination therapies and determine their sequence types (STs) and the mechanism of colistin resistance using whole-genome sequencing (WGS). (2) Methods: Antimicrobial susceptibility testing via broth microdilution against 12 antimicrobials from different classes and growth rate assays were performed. A checkerboard assay was conducted on PDR isolates using six different antimicrobials, each in combination with colistin. Genomic DNA was extracted from all isolates and subjected to WGS. (3) Results: All isolates were resistant to all tested antimicrobials with the one exception that was susceptible to gentamicin. Combining colistin with either meropenem, ceftolozane–tazobactam, or teicoplanin showed synergistic activity. Sequencing data revealed that 67% of the isolates belonged to Pasteur ST2 and 33% to ST187. Furthermore, these isolates harbored a number of resistance genes, including <i>bla</i><sub>OXA-23</sub>. Mutations in the <i>pmrC</i> gene were behind colistin resistance. (4) Conclusions: With the rise in antimicrobial resistance and the absence of novel antimicrobial production, alternative treatments must be found. The combination therapy results from this study suggest treatment options for PDR ST2 <i>A. baumannii</i>-infected patients. |
| first_indexed | 2024-03-07T22:20:31Z |
| format | Article |
| id | doaj.art-13f19d8850cb463ba74a6f5e4adc54e8 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-07T22:20:31Z |
| publishDate | 2024-02-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-13f19d8850cb463ba74a6f5e4adc54e82024-02-23T15:28:17ZengMDPI AGMicroorganisms2076-26072024-02-0112234910.3390/microorganisms12020349Molecular Characteristics of Colistin Resistance in <i>Acinetobacter baumannii</i> and the Activity of Antimicrobial Combination Therapy in a Tertiary Care Medical Center in LebanonAntoine Abou Fayad0Louis-Patrick Haraoui1Ahmad Sleiman2Hadi Hussein3Frédéric Grenier4Ghada Derbaj5Dana Itani6Sereen Iweir7Nour Sherri8Wael Bazzi9Sari Rasheed10Arax Tanelian11Mariam Miari12Bassam el Hafi13Souha S. Kanj14Zeina A. Kanafani15Ziad Daoud16George F. Araj17Ghassan M. Matar18Department of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, LebanonDepartment of Microbiology and Infectious Diseases, Faculty of Medicine and Health Sciences, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, CanadaDepartment of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, LebanonDepartment of Biology, Faculty of Science, Université de Sherbrooke, Sherbrooke, QC J1K 2R1, CanadaDepartment of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, LebanonCenter for Infectious Diseases Research, American University of Beirut, Beirut 1107 2020, LebanonCenter for Infectious Diseases Research, American University of Beirut, Beirut 1107 2020, LebanonLaboratory Department, My Michigan Health Midland Medical Center, College of Medicine, Central Michigan University, Saginaw, MI 48602, USACenter for Infectious Diseases Research, American University of Beirut, Beirut 1107 2020, LebanonDepartment of Experimental Pathology, Immunology and Microbiology, Faculty of Medicine, American University of Beirut, Beirut 1107 2020, Lebanon(1) Background: Infections with pan-drug-resistant (PDR) bacteria, such as <i>A. baumannii</i>, are becoming increasingly common, especially in healthcare facilities. In this study, we selected 15 colistin-resistant clinical <i>A. baumannii</i> isolates from a hospital in Beirut, Lebanon, to test combination therapies and determine their sequence types (STs) and the mechanism of colistin resistance using whole-genome sequencing (WGS). (2) Methods: Antimicrobial susceptibility testing via broth microdilution against 12 antimicrobials from different classes and growth rate assays were performed. A checkerboard assay was conducted on PDR isolates using six different antimicrobials, each in combination with colistin. Genomic DNA was extracted from all isolates and subjected to WGS. (3) Results: All isolates were resistant to all tested antimicrobials with the one exception that was susceptible to gentamicin. Combining colistin with either meropenem, ceftolozane–tazobactam, or teicoplanin showed synergistic activity. Sequencing data revealed that 67% of the isolates belonged to Pasteur ST2 and 33% to ST187. Furthermore, these isolates harbored a number of resistance genes, including <i>bla</i><sub>OXA-23</sub>. Mutations in the <i>pmrC</i> gene were behind colistin resistance. (4) Conclusions: With the rise in antimicrobial resistance and the absence of novel antimicrobial production, alternative treatments must be found. The combination therapy results from this study suggest treatment options for PDR ST2 <i>A. baumannii</i>-infected patients.https://www.mdpi.com/2076-2607/12/2/349antimicrobial resistance<i>Acinetobacter baumannii</i>colistin resistance |
| spellingShingle | Antoine Abou Fayad Louis-Patrick Haraoui Ahmad Sleiman Hadi Hussein Frédéric Grenier Ghada Derbaj Dana Itani Sereen Iweir Nour Sherri Wael Bazzi Sari Rasheed Arax Tanelian Mariam Miari Bassam el Hafi Souha S. Kanj Zeina A. Kanafani Ziad Daoud George F. Araj Ghassan M. Matar Molecular Characteristics of Colistin Resistance in <i>Acinetobacter baumannii</i> and the Activity of Antimicrobial Combination Therapy in a Tertiary Care Medical Center in Lebanon Microorganisms antimicrobial resistance <i>Acinetobacter baumannii</i> colistin resistance |
| title | Molecular Characteristics of Colistin Resistance in <i>Acinetobacter baumannii</i> and the Activity of Antimicrobial Combination Therapy in a Tertiary Care Medical Center in Lebanon |
| title_full | Molecular Characteristics of Colistin Resistance in <i>Acinetobacter baumannii</i> and the Activity of Antimicrobial Combination Therapy in a Tertiary Care Medical Center in Lebanon |
| title_fullStr | Molecular Characteristics of Colistin Resistance in <i>Acinetobacter baumannii</i> and the Activity of Antimicrobial Combination Therapy in a Tertiary Care Medical Center in Lebanon |
| title_full_unstemmed | Molecular Characteristics of Colistin Resistance in <i>Acinetobacter baumannii</i> and the Activity of Antimicrobial Combination Therapy in a Tertiary Care Medical Center in Lebanon |
| title_short | Molecular Characteristics of Colistin Resistance in <i>Acinetobacter baumannii</i> and the Activity of Antimicrobial Combination Therapy in a Tertiary Care Medical Center in Lebanon |
| title_sort | molecular characteristics of colistin resistance in i acinetobacter baumannii i and the activity of antimicrobial combination therapy in a tertiary care medical center in lebanon |
| topic | antimicrobial resistance <i>Acinetobacter baumannii</i> colistin resistance |
| url | https://www.mdpi.com/2076-2607/12/2/349 |
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