Banding pattern indicative of echinococcosis in a commercial cysticercosis western blot
<p>Abstract</p> <p>Objective</p> <p>A commercial cysticercosis Western blot was evaluated for serological cross-reactivity of sera from patients with alveolar (AE) and cystic echinococcosis (CE).</p> <p>Methods</p> <p>A total of 161 sera were exa...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-09-01
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| Series: | European Journal of Medical Research |
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| Online Access: | http://www.eurjmedres.com/content/14/10/451 |
| _version_ | 1818057531069562880 |
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| author | Tappe D Grüner B Kern P Frosch M |
| author_facet | Tappe D Grüner B Kern P Frosch M |
| author_sort | Tappe D |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Objective</p> <p>A commercial cysticercosis Western blot was evaluated for serological cross-reactivity of sera from patients with alveolar (AE) and cystic echinococcosis (CE).</p> <p>Methods</p> <p>A total of 161 sera were examined, including 31 sera from AE-patients, 11 sera from CE-patients, 9 sera from patients with other parasitic diseases and 109 sera from patients with unrelated medical conditions. All AE-and CE-sera were also examined by the echinococcosis Western blot.</p> <p>Results</p> <p>More sera from patients with AE than with CE showed cross-reactivity in the form of ladder-like patterns ("Mikado aspect") and untypical bands at 6-8 kDa (71% and 77.4% versus 27.3% and 45.5%, respectively). In contrast, triplets of bands in the area above 50 kDa and between 24 and 39-42 kDa were more frequent in CE than in AE sera. The fuzzy band at 50-55 kDa typical for cysticercosis was absent in all AE and CE sera.</p> <p>Conclusions</p> <p>Atypical banding patterns in the cysticercosis Western blot should raise the suspicion of a metacestode infection different from Taenia solium, i.e. Echinococcus multilocularis or E. granulosus, especially when the Mikado aspect and an altered 6-8 kDa band is visible in the absence of a fuzzy 50-55 kDa band.</p> |
| first_indexed | 2024-12-10T12:46:13Z |
| format | Article |
| id | doaj.art-13f3eddc21514d0b840d0aaf3a15493b |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-12-10T12:46:13Z |
| publishDate | 2009-09-01 |
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| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-13f3eddc21514d0b840d0aaf3a15493b2022-12-22T01:48:23ZengBMCEuropean Journal of Medical Research2047-783X2009-09-01141045110.1186/2047-783X-14-10-451Banding pattern indicative of echinococcosis in a commercial cysticercosis western blotTappe DGrüner BKern PFrosch M<p>Abstract</p> <p>Objective</p> <p>A commercial cysticercosis Western blot was evaluated for serological cross-reactivity of sera from patients with alveolar (AE) and cystic echinococcosis (CE).</p> <p>Methods</p> <p>A total of 161 sera were examined, including 31 sera from AE-patients, 11 sera from CE-patients, 9 sera from patients with other parasitic diseases and 109 sera from patients with unrelated medical conditions. All AE-and CE-sera were also examined by the echinococcosis Western blot.</p> <p>Results</p> <p>More sera from patients with AE than with CE showed cross-reactivity in the form of ladder-like patterns ("Mikado aspect") and untypical bands at 6-8 kDa (71% and 77.4% versus 27.3% and 45.5%, respectively). In contrast, triplets of bands in the area above 50 kDa and between 24 and 39-42 kDa were more frequent in CE than in AE sera. The fuzzy band at 50-55 kDa typical for cysticercosis was absent in all AE and CE sera.</p> <p>Conclusions</p> <p>Atypical banding patterns in the cysticercosis Western blot should raise the suspicion of a metacestode infection different from Taenia solium, i.e. Echinococcus multilocularis or E. granulosus, especially when the Mikado aspect and an altered 6-8 kDa band is visible in the absence of a fuzzy 50-55 kDa band.</p>http://www.eurjmedres.com/content/14/10/451Alveolar echinococcosisCystic echinococcosisCysticercosisWestern blotBanding patternSerologyCross-reactivity |
| spellingShingle | Tappe D Grüner B Kern P Frosch M Banding pattern indicative of echinococcosis in a commercial cysticercosis western blot European Journal of Medical Research Alveolar echinococcosis Cystic echinococcosis Cysticercosis Western blot Banding pattern Serology Cross-reactivity |
| title | Banding pattern indicative of echinococcosis in a commercial cysticercosis western blot |
| title_full | Banding pattern indicative of echinococcosis in a commercial cysticercosis western blot |
| title_fullStr | Banding pattern indicative of echinococcosis in a commercial cysticercosis western blot |
| title_full_unstemmed | Banding pattern indicative of echinococcosis in a commercial cysticercosis western blot |
| title_short | Banding pattern indicative of echinococcosis in a commercial cysticercosis western blot |
| title_sort | banding pattern indicative of echinococcosis in a commercial cysticercosis western blot |
| topic | Alveolar echinococcosis Cystic echinococcosis Cysticercosis Western blot Banding pattern Serology Cross-reactivity |
| url | http://www.eurjmedres.com/content/14/10/451 |
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