Recent Progresses in Development of Biosensors for Thrombin Detection
Thrombin is a serine protease with an essential role in homeostasis and blood coagulation. During vascular injuries, thrombin is generated from prothrombin, a plasma protein, to polymerize fibrinogen molecules into fibrin filaments. Moreover, thrombin is a potent stimulant for platelet activation, w...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/9/767 |
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| author | Reza Eivazzadeh-Keihan Zahra Saadatidizaji Ali Maleki Miguel de la Guardia Mohammad Mahdavi Sajjad Barzegar Samad Ahadian |
| author_facet | Reza Eivazzadeh-Keihan Zahra Saadatidizaji Ali Maleki Miguel de la Guardia Mohammad Mahdavi Sajjad Barzegar Samad Ahadian |
| author_sort | Reza Eivazzadeh-Keihan |
| collection | DOAJ |
| description | Thrombin is a serine protease with an essential role in homeostasis and blood coagulation. During vascular injuries, thrombin is generated from prothrombin, a plasma protein, to polymerize fibrinogen molecules into fibrin filaments. Moreover, thrombin is a potent stimulant for platelet activation, which causes blood clots to prevent bleeding. The rapid and sensitive detection of thrombin is important in biological analysis and clinical diagnosis. Hence, various biosensors for thrombin measurement have been developed. Biosensors are devices that produce a quantifiable signal from biological interactions in proportion to the concentration of a target analyte. An aptasensor is a biosensor in which a DNA or RNA aptamer has been used as a biological recognition element and can identify target molecules with a high degree of sensitivity and affinity. Designed biosensors could provide effective methods for the highly selective and specific detection of thrombin. This review has attempted to provide an update of the various biosensors proposed in the literature, which have been designed for thrombin detection. According to their various transducers, the constructions and compositions, the performance, benefits, and restrictions of each are summarized and compared. |
| first_indexed | 2024-03-10T00:34:08Z |
| format | Article |
| id | doaj.art-13f4bb2c8b8a44c3acf1d2573eeff24f |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T00:34:08Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-13f4bb2c8b8a44c3acf1d2573eeff24f2023-11-23T15:18:49ZengMDPI AGBiosensors2079-63742022-09-0112976710.3390/bios12090767Recent Progresses in Development of Biosensors for Thrombin DetectionReza Eivazzadeh-Keihan0Zahra Saadatidizaji1Ali Maleki2Miguel de la Guardia3Mohammad Mahdavi4Sajjad Barzegar5Samad Ahadian6Catalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, IranCatalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, IranCatalysts and Organic Synthesis Research Laboratory, Department of Chemistry, Iran University of Science and Technology, Tehran 16846-13114, IranDepartment of Analytical Chemistry, University of Valencia, Dr. Moliner 50, 46100 Valencia, SpainEndocrinology and Metabolism Research Center, Endocrinology and Metabolism Clinical Sciences Institute, Tehran University of Medical Sciences, Tehran 14117-13139, IranDepartment of Chemical Engineering, School of Chemical and Petroleum Engineering, Shiraz University, Shiraz 71348-51154, IranNouBio, Los Angeles, CA 90025, USAThrombin is a serine protease with an essential role in homeostasis and blood coagulation. During vascular injuries, thrombin is generated from prothrombin, a plasma protein, to polymerize fibrinogen molecules into fibrin filaments. Moreover, thrombin is a potent stimulant for platelet activation, which causes blood clots to prevent bleeding. The rapid and sensitive detection of thrombin is important in biological analysis and clinical diagnosis. Hence, various biosensors for thrombin measurement have been developed. Biosensors are devices that produce a quantifiable signal from biological interactions in proportion to the concentration of a target analyte. An aptasensor is a biosensor in which a DNA or RNA aptamer has been used as a biological recognition element and can identify target molecules with a high degree of sensitivity and affinity. Designed biosensors could provide effective methods for the highly selective and specific detection of thrombin. This review has attempted to provide an update of the various biosensors proposed in the literature, which have been designed for thrombin detection. According to their various transducers, the constructions and compositions, the performance, benefits, and restrictions of each are summarized and compared.https://www.mdpi.com/2079-6374/12/9/767thrombinbiosensoraptameroptical biosensorelectrochemical biosensordetection |
| spellingShingle | Reza Eivazzadeh-Keihan Zahra Saadatidizaji Ali Maleki Miguel de la Guardia Mohammad Mahdavi Sajjad Barzegar Samad Ahadian Recent Progresses in Development of Biosensors for Thrombin Detection Biosensors thrombin biosensor aptamer optical biosensor electrochemical biosensor detection |
| title | Recent Progresses in Development of Biosensors for Thrombin Detection |
| title_full | Recent Progresses in Development of Biosensors for Thrombin Detection |
| title_fullStr | Recent Progresses in Development of Biosensors for Thrombin Detection |
| title_full_unstemmed | Recent Progresses in Development of Biosensors for Thrombin Detection |
| title_short | Recent Progresses in Development of Biosensors for Thrombin Detection |
| title_sort | recent progresses in development of biosensors for thrombin detection |
| topic | thrombin biosensor aptamer optical biosensor electrochemical biosensor detection |
| url | https://www.mdpi.com/2079-6374/12/9/767 |
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