Photodynamic Treatment of Human Breast and Prostate Cancer Cells Using Rose Bengal-Encapsulated Nanoparticles
Cancer, a prominent cause of death, presents treatment challenges, including high dosage requirements, drug resistance, poor tumour penetration and systemic toxicity in traditional chemotherapy. Photodynamic therapy, using photosensitizers like rose bengal (RB) with a green laser, shows promise agai...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/19/6901 |
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| author | Mir Muhammad Nasir Uddin Alina Bekmukhametova Anu Antony Shital K. Barman Jessica Houang Ming J. Wu James Hook Laurel George Richard Wuhrer Damia Mawad Daniel Ta Antonio Lauto |
| author_facet | Mir Muhammad Nasir Uddin Alina Bekmukhametova Anu Antony Shital K. Barman Jessica Houang Ming J. Wu James Hook Laurel George Richard Wuhrer Damia Mawad Daniel Ta Antonio Lauto |
| author_sort | Mir Muhammad Nasir Uddin |
| collection | DOAJ |
| description | Cancer, a prominent cause of death, presents treatment challenges, including high dosage requirements, drug resistance, poor tumour penetration and systemic toxicity in traditional chemotherapy. Photodynamic therapy, using photosensitizers like rose bengal (RB) with a green laser, shows promise against breast cancer cells in vitro. However, the hydrophilic RB struggles to efficiently penetrate the tumour site due to the unique clinical microenvironment, aggregating around rather than entering cancer cells. In this study, we have synthesized and characterized RB-encapsulated chitosan nanoparticles with a peak particle size of ~200 nm. These nanoparticles are readily internalized by cells and, in combination with a green laser (λ = 532 nm) killed 94–98% of cultured human breast cancer cells (MCF-7) and prostate cancer cells (PC3) at a low dosage (25 μg/mL RB-nanoparticles, fluence ~126 J/cm<sup>2</sup>, and irradiance ~0.21 W/cm<sup>2</sup>). Furthermore, these nanoparticles are not toxic to cultured human normal breast cells (MCF10A), which opens an avenue for translational applications. |
| first_indexed | 2024-03-10T21:39:22Z |
| format | Article |
| id | doaj.art-13f8aea04c8d45d59fc8d150fe43aac9 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T21:39:22Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-13f8aea04c8d45d59fc8d150fe43aac92023-11-19T14:47:06ZengMDPI AGMolecules1420-30492023-10-012819690110.3390/molecules28196901Photodynamic Treatment of Human Breast and Prostate Cancer Cells Using Rose Bengal-Encapsulated NanoparticlesMir Muhammad Nasir Uddin0Alina Bekmukhametova1Anu Antony2Shital K. Barman3Jessica Houang4Ming J. Wu5James Hook6Laurel George7Richard Wuhrer8Damia Mawad9Daniel Ta10Antonio Lauto11School of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Chemistry, University of New South Wales, Sydney, NSW 2052, AustraliaAdvanced Materials Characterisation Facility, Western Sydney University, Penrith, NSW 2750, AustraliaAdvanced Materials Characterisation Facility, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Materials Science and Engineering and Australian Centre for NanoMedicine, University of New South Wales, Kensington, NSW 2052, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaCancer, a prominent cause of death, presents treatment challenges, including high dosage requirements, drug resistance, poor tumour penetration and systemic toxicity in traditional chemotherapy. Photodynamic therapy, using photosensitizers like rose bengal (RB) with a green laser, shows promise against breast cancer cells in vitro. However, the hydrophilic RB struggles to efficiently penetrate the tumour site due to the unique clinical microenvironment, aggregating around rather than entering cancer cells. In this study, we have synthesized and characterized RB-encapsulated chitosan nanoparticles with a peak particle size of ~200 nm. These nanoparticles are readily internalized by cells and, in combination with a green laser (λ = 532 nm) killed 94–98% of cultured human breast cancer cells (MCF-7) and prostate cancer cells (PC3) at a low dosage (25 μg/mL RB-nanoparticles, fluence ~126 J/cm<sup>2</sup>, and irradiance ~0.21 W/cm<sup>2</sup>). Furthermore, these nanoparticles are not toxic to cultured human normal breast cells (MCF10A), which opens an avenue for translational applications.https://www.mdpi.com/1420-3049/28/19/6901tumorsreactive oxygen specieslasers |
| spellingShingle | Mir Muhammad Nasir Uddin Alina Bekmukhametova Anu Antony Shital K. Barman Jessica Houang Ming J. Wu James Hook Laurel George Richard Wuhrer Damia Mawad Daniel Ta Antonio Lauto Photodynamic Treatment of Human Breast and Prostate Cancer Cells Using Rose Bengal-Encapsulated Nanoparticles Molecules tumors reactive oxygen species lasers |
| title | Photodynamic Treatment of Human Breast and Prostate Cancer Cells Using Rose Bengal-Encapsulated Nanoparticles |
| title_full | Photodynamic Treatment of Human Breast and Prostate Cancer Cells Using Rose Bengal-Encapsulated Nanoparticles |
| title_fullStr | Photodynamic Treatment of Human Breast and Prostate Cancer Cells Using Rose Bengal-Encapsulated Nanoparticles |
| title_full_unstemmed | Photodynamic Treatment of Human Breast and Prostate Cancer Cells Using Rose Bengal-Encapsulated Nanoparticles |
| title_short | Photodynamic Treatment of Human Breast and Prostate Cancer Cells Using Rose Bengal-Encapsulated Nanoparticles |
| title_sort | photodynamic treatment of human breast and prostate cancer cells using rose bengal encapsulated nanoparticles |
| topic | tumors reactive oxygen species lasers |
| url | https://www.mdpi.com/1420-3049/28/19/6901 |
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