Mutation of <i>Inositol 1,3,4-trisphosphate 5/6-kinase6</i> Impairs Plant Growth and Phytic Acid Synthesis in Rice
Inositol 1,3,4-trisphosphate 5/6-kinase (ITPK) is encoded by six genes in rice (<i>OsITPK1-6</i>). A previous study had shown that nucleotide substitutions of <i>OsITPK6</i> could significantly lower the phytic acid content in rice grains. In the present study, the possibilit...
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MDPI AG
2019-04-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/8/5/114 |
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| author | Meng Jiang Yang Liu Yanhua Liu Yuanyuan Tan Jianzhong Huang Qingyao Shu |
| author_facet | Meng Jiang Yang Liu Yanhua Liu Yuanyuan Tan Jianzhong Huang Qingyao Shu |
| author_sort | Meng Jiang |
| collection | DOAJ |
| description | Inositol 1,3,4-trisphosphate 5/6-kinase (ITPK) is encoded by six genes in rice (<i>OsITPK1-6</i>). A previous study had shown that nucleotide substitutions of <i>OsITPK6</i> could significantly lower the phytic acid content in rice grains. In the present study, the possibility of establishing a genome editing-based method for breeding low-phytic acid cultivars in rice was explored, in conjunction with the functional determination of OsITPK6. Four <i>OsITPK6</i> mutant lines were generated by targeted mutagenesis of the gene’s first exon using the CRISPR/Cas9 method, one (<i>ositpk6_1</i>) with a 6-bp in-frame deletion, and other three with frameshift mutations (<i>ositpk6_2</i>, _<i>3</i>, and _<i>4</i>). The frameshift mutations severely impaired plant growth and reproduction, while the effect of <i>ositpk6_1</i> was relatively limited. The mutant lines <i>ositpk6_1</i> and _<i>2</i> had significantly lower levels (−10.1% and −32.1%) of phytic acid and higher levels (4.12- and 5.18-fold) of inorganic phosphorus compared with the wild-type (WT) line. The line <i>ositpk6_1</i> also showed less tolerance to osmotic stresses. Our research demonstrates that mutations of <i>OsITPK6</i>, while effectively reducing phytic acid biosynthesis in rice grain, could significantly impair plant growth and reproduction. |
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| last_indexed | 2024-04-11T16:34:27Z |
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| spelling | doaj.art-13fed8e5df2e4fda88bd98644b7c947c2022-12-22T04:13:52ZengMDPI AGPlants2223-77472019-04-018511410.3390/plants8050114plants8050114Mutation of <i>Inositol 1,3,4-trisphosphate 5/6-kinase6</i> Impairs Plant Growth and Phytic Acid Synthesis in RiceMeng Jiang0Yang Liu1Yanhua Liu2Yuanyuan Tan3Jianzhong Huang4Qingyao Shu5National Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310058, ChinaNational Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310058, ChinaNational Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310058, ChinaNational Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310058, ChinaNational Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310058, ChinaNational Key Laboratory of Rice Biology, Institute of Crop Sciences, Zhejiang University, Hangzhou 310058, ChinaInositol 1,3,4-trisphosphate 5/6-kinase (ITPK) is encoded by six genes in rice (<i>OsITPK1-6</i>). A previous study had shown that nucleotide substitutions of <i>OsITPK6</i> could significantly lower the phytic acid content in rice grains. In the present study, the possibility of establishing a genome editing-based method for breeding low-phytic acid cultivars in rice was explored, in conjunction with the functional determination of OsITPK6. Four <i>OsITPK6</i> mutant lines were generated by targeted mutagenesis of the gene’s first exon using the CRISPR/Cas9 method, one (<i>ositpk6_1</i>) with a 6-bp in-frame deletion, and other three with frameshift mutations (<i>ositpk6_2</i>, _<i>3</i>, and _<i>4</i>). The frameshift mutations severely impaired plant growth and reproduction, while the effect of <i>ositpk6_1</i> was relatively limited. The mutant lines <i>ositpk6_1</i> and _<i>2</i> had significantly lower levels (−10.1% and −32.1%) of phytic acid and higher levels (4.12- and 5.18-fold) of inorganic phosphorus compared with the wild-type (WT) line. The line <i>ositpk6_1</i> also showed less tolerance to osmotic stresses. Our research demonstrates that mutations of <i>OsITPK6</i>, while effectively reducing phytic acid biosynthesis in rice grain, could significantly impair plant growth and reproduction.https://www.mdpi.com/2223-7747/8/5/114genome editinggrowth<i>ositpk6</i>phytic acidrice |
| spellingShingle | Meng Jiang Yang Liu Yanhua Liu Yuanyuan Tan Jianzhong Huang Qingyao Shu Mutation of <i>Inositol 1,3,4-trisphosphate 5/6-kinase6</i> Impairs Plant Growth and Phytic Acid Synthesis in Rice Plants genome editing growth <i>ositpk6</i> phytic acid rice |
| title | Mutation of <i>Inositol 1,3,4-trisphosphate 5/6-kinase6</i> Impairs Plant Growth and Phytic Acid Synthesis in Rice |
| title_full | Mutation of <i>Inositol 1,3,4-trisphosphate 5/6-kinase6</i> Impairs Plant Growth and Phytic Acid Synthesis in Rice |
| title_fullStr | Mutation of <i>Inositol 1,3,4-trisphosphate 5/6-kinase6</i> Impairs Plant Growth and Phytic Acid Synthesis in Rice |
| title_full_unstemmed | Mutation of <i>Inositol 1,3,4-trisphosphate 5/6-kinase6</i> Impairs Plant Growth and Phytic Acid Synthesis in Rice |
| title_short | Mutation of <i>Inositol 1,3,4-trisphosphate 5/6-kinase6</i> Impairs Plant Growth and Phytic Acid Synthesis in Rice |
| title_sort | mutation of i inositol 1 3 4 trisphosphate 5 6 kinase6 i impairs plant growth and phytic acid synthesis in rice |
| topic | genome editing growth <i>ositpk6</i> phytic acid rice |
| url | https://www.mdpi.com/2223-7747/8/5/114 |
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