Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity
Summary: Organoids can enable the study of solid tumors initiated from a single cancer stem cell (CSC) ex vivo. We describe a serial tumor organoid plating protocol using primary colorectal cancer (CRC) tissues as a rapid and cost-efficient approach to evaluate the impact of therapeutic intervention...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722000983 |
| _version_ | 1818646638969749504 |
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| author | Christopher J. Bergin Yannick D. Benoit |
| author_facet | Christopher J. Bergin Yannick D. Benoit |
| author_sort | Christopher J. Bergin |
| collection | DOAJ |
| description | Summary: Organoids can enable the study of solid tumors initiated from a single cancer stem cell (CSC) ex vivo. We describe a serial tumor organoid plating protocol using primary colorectal cancer (CRC) tissues as a rapid and cost-efficient approach to evaluate the impact of therapeutic interventions on CSC functions. We detail the isolation of primary colorectal CSCs, organoid embedding, serial passaging, and CSC-related analytical techniques.For complete details on the use and execution of this protocol, please refer to Masibag et al. (2021) and Bergin et al. (2021). |
| first_indexed | 2024-12-17T00:49:50Z |
| format | Article |
| id | doaj.art-13ffa8b9edfe485a911ba589a6d88828 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-17T00:49:50Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-13ffa8b9edfe485a911ba589a6d888282022-12-21T22:09:48ZengElsevierSTAR Protocols2666-16672022-03-0131101218Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activityChristopher J. Bergin0Yannick D. Benoit1Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Corresponding authorDepartment of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; Corresponding authorSummary: Organoids can enable the study of solid tumors initiated from a single cancer stem cell (CSC) ex vivo. We describe a serial tumor organoid plating protocol using primary colorectal cancer (CRC) tissues as a rapid and cost-efficient approach to evaluate the impact of therapeutic interventions on CSC functions. We detail the isolation of primary colorectal CSCs, organoid embedding, serial passaging, and CSC-related analytical techniques.For complete details on the use and execution of this protocol, please refer to Masibag et al. (2021) and Bergin et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166722000983Cell isolationSingle CellCell-based AssaysCancerHealth SciencesMicroscopy |
| spellingShingle | Christopher J. Bergin Yannick D. Benoit Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity STAR Protocols Cell isolation Single Cell Cell-based Assays Cancer Health Sciences Microscopy |
| title | Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity |
| title_full | Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity |
| title_fullStr | Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity |
| title_full_unstemmed | Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity |
| title_short | Protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity |
| title_sort | protocol for serial organoid formation assay using primary colorectal cancer tissues to evaluate cancer stem cell activity |
| topic | Cell isolation Single Cell Cell-based Assays Cancer Health Sciences Microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722000983 |
| work_keys_str_mv | AT christopherjbergin protocolforserialorganoidformationassayusingprimarycolorectalcancertissuestoevaluatecancerstemcellactivity AT yannickdbenoit protocolforserialorganoidformationassayusingprimarycolorectalcancertissuestoevaluatecancerstemcellactivity |