Development of a Transformation System for the Medicinal Fungus Sanghuangporus baumii and Acquisition of High-Value Strain
AbstractTo further explore the molecular mechanism of triterpenoid biosynthesis and acquire high-value strain of Sanghuangporus baumii, the Agrobacterium tumefaciens-mediated transformation (ATMT) system was studied. The key triterpenoid biosynthesis-associated gene isopentenyl diphosphate isomerase...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-05-01
|
| Series: | Mycobiology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/12298093.2023.2220164 |
| _version_ | 1797797779948437504 |
|---|---|
| author | Zengcai Liu Ruipeng Liu Li Zou |
| author_facet | Zengcai Liu Ruipeng Liu Li Zou |
| author_sort | Zengcai Liu |
| collection | DOAJ |
| description | AbstractTo further explore the molecular mechanism of triterpenoid biosynthesis and acquire high-value strain of Sanghuangporus baumii, the Agrobacterium tumefaciens-mediated transformation (ATMT) system was studied. The key triterpenoid biosynthesis-associated gene isopentenyl diphosphate isomerase (IDI) was transformed into S. baumii by ATMT system. Then, the qRT-PCR technique was used to analyze gene transcript level, and the widely targeted metabolomics was used to investigate individual triterpenoid content. Total triterpenoid content and anti-oxidant activity were determined by spectrophotometer. In this study, we for the first time established an efficient ATMT system and transferred the IDI gene into S. baumii. Relative to the wild-type (WT) strain, the IDI-transformant (IT) strain showed significantly higher transcript levels of IDI and total triterpenoid content. We then investigated individual triterpenoids in S. baumii, which led to the identification of 10 distinct triterpenoids. The contents of individual triterpenoids produced by the IT2 strain were 1.76–10.03 times higher than those produced by the WT strain. The triterpenoid production showed a significant positive correlation with the IDI gene expression. Besides, IT2 strain showed better anti-oxidant activity. The findings provide valuable information about the biosynthetic pathway of triterpenoids and provide a strategy for cultivating high-value S. baumii strains. |
| first_indexed | 2024-03-13T03:53:32Z |
| format | Article |
| id | doaj.art-1402dd245b01455ca9e4510338cb04ef |
| institution | Directory Open Access Journal |
| issn | 1229-8093 2092-9323 |
| language | English |
| last_indexed | 2024-03-13T03:53:32Z |
| publishDate | 2023-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycobiology |
| spelling | doaj.art-1402dd245b01455ca9e4510338cb04ef2023-06-22T08:04:21ZengTaylor & Francis GroupMycobiology1229-80932092-93232023-05-0151316917710.1080/12298093.2023.2220164Development of a Transformation System for the Medicinal Fungus Sanghuangporus baumii and Acquisition of High-Value StrainZengcai Liu0Ruipeng Liu1Li Zou2College of Forestry, Northeast Forestry University, Harbin, ChinaCollege of Forestry, Northeast Forestry University, Harbin, ChinaCollege of Forestry, Northeast Forestry University, Harbin, ChinaAbstractTo further explore the molecular mechanism of triterpenoid biosynthesis and acquire high-value strain of Sanghuangporus baumii, the Agrobacterium tumefaciens-mediated transformation (ATMT) system was studied. The key triterpenoid biosynthesis-associated gene isopentenyl diphosphate isomerase (IDI) was transformed into S. baumii by ATMT system. Then, the qRT-PCR technique was used to analyze gene transcript level, and the widely targeted metabolomics was used to investigate individual triterpenoid content. Total triterpenoid content and anti-oxidant activity were determined by spectrophotometer. In this study, we for the first time established an efficient ATMT system and transferred the IDI gene into S. baumii. Relative to the wild-type (WT) strain, the IDI-transformant (IT) strain showed significantly higher transcript levels of IDI and total triterpenoid content. We then investigated individual triterpenoids in S. baumii, which led to the identification of 10 distinct triterpenoids. The contents of individual triterpenoids produced by the IT2 strain were 1.76–10.03 times higher than those produced by the WT strain. The triterpenoid production showed a significant positive correlation with the IDI gene expression. Besides, IT2 strain showed better anti-oxidant activity. The findings provide valuable information about the biosynthetic pathway of triterpenoids and provide a strategy for cultivating high-value S. baumii strains.https://www.tandfonline.com/doi/10.1080/12298093.2023.2220164Sanghuangporus baumiiisopentenyl diphosphate isomerasegenetic transformationtriterpenoidsantioxidant |
| spellingShingle | Zengcai Liu Ruipeng Liu Li Zou Development of a Transformation System for the Medicinal Fungus Sanghuangporus baumii and Acquisition of High-Value Strain Mycobiology Sanghuangporus baumii isopentenyl diphosphate isomerase genetic transformation triterpenoids antioxidant |
| title | Development of a Transformation System for the Medicinal Fungus Sanghuangporus baumii and Acquisition of High-Value Strain |
| title_full | Development of a Transformation System for the Medicinal Fungus Sanghuangporus baumii and Acquisition of High-Value Strain |
| title_fullStr | Development of a Transformation System for the Medicinal Fungus Sanghuangporus baumii and Acquisition of High-Value Strain |
| title_full_unstemmed | Development of a Transformation System for the Medicinal Fungus Sanghuangporus baumii and Acquisition of High-Value Strain |
| title_short | Development of a Transformation System for the Medicinal Fungus Sanghuangporus baumii and Acquisition of High-Value Strain |
| title_sort | development of a transformation system for the medicinal fungus sanghuangporus baumii and acquisition of high value strain |
| topic | Sanghuangporus baumii isopentenyl diphosphate isomerase genetic transformation triterpenoids antioxidant |
| url | https://www.tandfonline.com/doi/10.1080/12298093.2023.2220164 |
| work_keys_str_mv | AT zengcailiu developmentofatransformationsystemforthemedicinalfungussanghuangporusbaumiiandacquisitionofhighvaluestrain AT ruipengliu developmentofatransformationsystemforthemedicinalfungussanghuangporusbaumiiandacquisitionofhighvaluestrain AT lizou developmentofatransformationsystemforthemedicinalfungussanghuangporusbaumiiandacquisitionofhighvaluestrain |