Investigating the Effect of Drugs of Chloramphenicol and Gentamicin on Acanthamoeba genotype T3 Causing keratitis Isolated from Environmental samples in vitro
This study is an investigation of the drugs effect on some pathogenic Acanthamoeba isolated from Iraqi waters, where the problem of environmental adaptation that characterizes this organism in addition to being a reservoir for many pathogenic microorganisms that take shelter in it to escape disinfe...
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| Format: | Article |
| Language: | Arabic |
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College of Science for Women, University of Baghdad
2022-06-01
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| Series: | Baghdad Science Journal |
| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/5480 |
| _version_ | 1817977419880988672 |
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| author | Turkan K. Karyagdi Husain F. Hassan Shihab A. Mohammed |
| author_facet | Turkan K. Karyagdi Husain F. Hassan Shihab A. Mohammed |
| author_sort | Turkan K. Karyagdi |
| collection | DOAJ |
| description |
This study is an investigation of the drugs effect on some pathogenic Acanthamoeba isolated from Iraqi waters, where the problem of environmental adaptation that characterizes this organism in addition to being a reservoir for many pathogenic microorganisms that take shelter in it to escape disinfectants and medicines is sometimes difficult to treat it with traditional treatments. Twenty water samples were collected from different water regions in Iraq, namely the Dokan Lake, Tigris River, Euphrates River and Najaf Sea, 5 samples from each source. Acanthamoeba was isolated from water samples on NNA and PYG media, using an inverted microscope with an electron microscope to determine their phenotypic features. PCR and Sequencing were also used to determine their genotype. The isolates were belonged to the T3 genotype that causes corneal infections. Then two types of drugs were used to treat it, which were Chloramphenicol and Gentamicin at three different concentrations, 0.1%, 0.3% and 0.6% and their inhibitory effect was compared with each other and with the positive control of the Chlorohexidine drug at a concentration of 0.2% which was recommended to treat Acanthamoeba keratitis. The results showed that both drugs have an inhibitory effect against Acanthamoeba growth, and that the chloramphenicol had more effect to inhibit compared with gentamicin and chlorhexidine, and it can be used as an alternative treatment instead of chlorohexidine for treating Acanthamoeba keratitis.
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| first_indexed | 2024-04-13T22:16:10Z |
| format | Article |
| id | doaj.art-14039aa065964608813a9c57c9c77dc1 |
| institution | Directory Open Access Journal |
| issn | 2078-8665 2411-7986 |
| language | Arabic |
| last_indexed | 2024-04-13T22:16:10Z |
| publishDate | 2022-06-01 |
| publisher | College of Science for Women, University of Baghdad |
| record_format | Article |
| series | Baghdad Science Journal |
| spelling | doaj.art-14039aa065964608813a9c57c9c77dc12022-12-22T02:27:31ZaraCollege of Science for Women, University of BaghdadBaghdad Science Journal2078-86652411-79862022-06-0119310.21123/bsj.2022.19.3.0493Investigating the Effect of Drugs of Chloramphenicol and Gentamicin on Acanthamoeba genotype T3 Causing keratitis Isolated from Environmental samples in vitroTurkan K. Karyagdi0Husain F. Hassan1Shihab A. Mohammed2Department of Biology, Tikrit University, College of Education for Pure Sciences ,Iraq.Department of Parasitology, Kirkuk University, College of Science, Iraq.Department of Parasitology, Tikrit University, College of Education for Pure Sciences, Iraq. This study is an investigation of the drugs effect on some pathogenic Acanthamoeba isolated from Iraqi waters, where the problem of environmental adaptation that characterizes this organism in addition to being a reservoir for many pathogenic microorganisms that take shelter in it to escape disinfectants and medicines is sometimes difficult to treat it with traditional treatments. Twenty water samples were collected from different water regions in Iraq, namely the Dokan Lake, Tigris River, Euphrates River and Najaf Sea, 5 samples from each source. Acanthamoeba was isolated from water samples on NNA and PYG media, using an inverted microscope with an electron microscope to determine their phenotypic features. PCR and Sequencing were also used to determine their genotype. The isolates were belonged to the T3 genotype that causes corneal infections. Then two types of drugs were used to treat it, which were Chloramphenicol and Gentamicin at three different concentrations, 0.1%, 0.3% and 0.6% and their inhibitory effect was compared with each other and with the positive control of the Chlorohexidine drug at a concentration of 0.2% which was recommended to treat Acanthamoeba keratitis. The results showed that both drugs have an inhibitory effect against Acanthamoeba growth, and that the chloramphenicol had more effect to inhibit compared with gentamicin and chlorhexidine, and it can be used as an alternative treatment instead of chlorohexidine for treating Acanthamoeba keratitis. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/5480 |
| spellingShingle | Turkan K. Karyagdi Husain F. Hassan Shihab A. Mohammed Investigating the Effect of Drugs of Chloramphenicol and Gentamicin on Acanthamoeba genotype T3 Causing keratitis Isolated from Environmental samples in vitro Baghdad Science Journal |
| title | Investigating the Effect of Drugs of Chloramphenicol and Gentamicin on Acanthamoeba genotype T3 Causing keratitis Isolated from Environmental samples in vitro |
| title_full | Investigating the Effect of Drugs of Chloramphenicol and Gentamicin on Acanthamoeba genotype T3 Causing keratitis Isolated from Environmental samples in vitro |
| title_fullStr | Investigating the Effect of Drugs of Chloramphenicol and Gentamicin on Acanthamoeba genotype T3 Causing keratitis Isolated from Environmental samples in vitro |
| title_full_unstemmed | Investigating the Effect of Drugs of Chloramphenicol and Gentamicin on Acanthamoeba genotype T3 Causing keratitis Isolated from Environmental samples in vitro |
| title_short | Investigating the Effect of Drugs of Chloramphenicol and Gentamicin on Acanthamoeba genotype T3 Causing keratitis Isolated from Environmental samples in vitro |
| title_sort | investigating the effect of drugs of chloramphenicol and gentamicin on acanthamoeba genotype t3 causing keratitis isolated from environmental samples in vitro |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/5480 |
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