Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo.
Retroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundant...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-04-01
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| Series: | PLoS Pathogens |
| Online Access: | http://europepmc.org/articles/PMC4849745?pdf=render |
| _version_ | 1818057617529896960 |
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| author | Nicolas A Gillet Malik Hamaidia Alix de Brogniez Gerónimo Gutiérrez Nathalie Renotte Michal Reichert Karina Trono Luc Willems |
| author_facet | Nicolas A Gillet Malik Hamaidia Alix de Brogniez Gerónimo Gutiérrez Nathalie Renotte Michal Reichert Karina Trono Luc Willems |
| author_sort | Nicolas A Gillet |
| collection | DOAJ |
| description | Retroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundantly expressed in B-cell tumors in the absence of significant levels of genomic and subgenomic viral RNAs. Using deep RNA sequencing and functional reporter assays, we show that miRNAs mediate the expression of genes involved in cell signaling, cancer and immunity. We further demonstrate that BLV miRNAs are essential to induce B-cell tumors in an experimental model and to promote efficient viral replication in the natural host. |
| first_indexed | 2024-12-10T12:47:35Z |
| format | Article |
| id | doaj.art-1404756b603e42cbb47d3eeeef0e8db5 |
| institution | Directory Open Access Journal |
| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-12-10T12:47:35Z |
| publishDate | 2016-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj.art-1404756b603e42cbb47d3eeeef0e8db52022-12-22T01:48:21ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742016-04-01124e100558810.1371/journal.ppat.1005588Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo.Nicolas A GilletMalik HamaidiaAlix de BrogniezGerónimo GutiérrezNathalie RenotteMichal ReichertKarina TronoLuc WillemsRetroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundantly expressed in B-cell tumors in the absence of significant levels of genomic and subgenomic viral RNAs. Using deep RNA sequencing and functional reporter assays, we show that miRNAs mediate the expression of genes involved in cell signaling, cancer and immunity. We further demonstrate that BLV miRNAs are essential to induce B-cell tumors in an experimental model and to promote efficient viral replication in the natural host.http://europepmc.org/articles/PMC4849745?pdf=render |
| spellingShingle | Nicolas A Gillet Malik Hamaidia Alix de Brogniez Gerónimo Gutiérrez Nathalie Renotte Michal Reichert Karina Trono Luc Willems Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo. PLoS Pathogens |
| title | Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo. |
| title_full | Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo. |
| title_fullStr | Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo. |
| title_full_unstemmed | Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo. |
| title_short | Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo. |
| title_sort | bovine leukemia virus small noncoding rnas are functional elements that regulate replication and contribute to oncogenesis in vivo |
| url | http://europepmc.org/articles/PMC4849745?pdf=render |
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