Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to Fumonisins
Animal feed is very frequently contaminated with different types of mold, the metabolites of which are toxic to living organisms. Mold-contaminated cereal is rich in heat-resistant and harmful metabolites such as fumonisins (FBs). The amount of FBs consumed as part of animal feed, including livestoc...
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MDPI AG
2023-05-01
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| author | Ewa Tomaszewska Halyna Rudyk Piotr Dobrowolski Marcin B. Arciszewski Janine Donaldson Katarzyna Kras Beata Abramowicz Damian Kuc Siemowit Muszyński |
| author_facet | Ewa Tomaszewska Halyna Rudyk Piotr Dobrowolski Marcin B. Arciszewski Janine Donaldson Katarzyna Kras Beata Abramowicz Damian Kuc Siemowit Muszyński |
| author_sort | Ewa Tomaszewska |
| collection | DOAJ |
| description | Animal feed is very frequently contaminated with different types of mold, the metabolites of which are toxic to living organisms. Mold-contaminated cereal is rich in heat-resistant and harmful metabolites such as fumonisins (FBs). The amount of FBs consumed as part of animal feed, including livestock feed, is unknown. Therefore, this study aimed to evaluate the effects of maternal oral FB intoxication on basal duodenum morphology and the immunolocalization of gut hormones responsible for food intake (leptin and ghrelin), as well as their receptors, in newborn rat offspring. Pregnant Wistar rats were randomly allocated to one of three groups: a control group or one of two FB-intoxicated groups (60 or 90 mg FB/kg b.w., respectively). Basal morphological duodenal parameters changed in a dose- and sex-dependent manner. The intensity of the ghrelin immunoreaction was unchanged in females, while in males it increased after FB exposure (60 mg/kg b.w.), with a simultaneous decrease in expression of the ghrelin receptor. Leptin and its receptor immunoreaction intensity was decreased in both sexes following FB exposure. The current study highlighted the potential involvement of intestinal ghrelin and leptin in the metabolic disturbances observed later in life in offspring that were prenatally exposed to fumonisins. |
| first_indexed | 2024-03-11T04:24:50Z |
| format | Article |
| id | doaj.art-1409a858ad7348b2b168f9e7ade59de9 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T04:24:50Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-1409a858ad7348b2b168f9e7ade59de92023-11-17T22:30:29ZengMDPI AGAnimals2076-26152023-05-01139153810.3390/ani13091538Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to FumonisinsEwa Tomaszewska0Halyna Rudyk1Piotr Dobrowolski2Marcin B. Arciszewski3Janine Donaldson4Katarzyna Kras5Beata Abramowicz6Damian Kuc7Siemowit Muszyński8Department of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, PolandDepartment of Animal Physiology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, PolandDepartment of Functional Anatomy and Cytobiology, Faculty of Biology and Biotechnology, Maria Curie-Sklodowska University, 20-033 Lublin, PolandDepartment of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, PolandSchool of Physiology, Faculty of Health Sciences, University of the Witwatersrand, Parktown, Johannesburg 2193, South AfricaDepartment of Animal Anatomy and Histology, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-950 Lublin, PolandDepartment and Clinic of Animal Internal Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, PolandChair and Department of Developmental Dentistry, Medical University of Lublin, 20-081 Lublin, PolandDepartment of Biophysics, Faculty of Environmental Biology, University of Life Sciences in Lublin, 20-950 Lublin, PolandAnimal feed is very frequently contaminated with different types of mold, the metabolites of which are toxic to living organisms. Mold-contaminated cereal is rich in heat-resistant and harmful metabolites such as fumonisins (FBs). The amount of FBs consumed as part of animal feed, including livestock feed, is unknown. Therefore, this study aimed to evaluate the effects of maternal oral FB intoxication on basal duodenum morphology and the immunolocalization of gut hormones responsible for food intake (leptin and ghrelin), as well as their receptors, in newborn rat offspring. Pregnant Wistar rats were randomly allocated to one of three groups: a control group or one of two FB-intoxicated groups (60 or 90 mg FB/kg b.w., respectively). Basal morphological duodenal parameters changed in a dose- and sex-dependent manner. The intensity of the ghrelin immunoreaction was unchanged in females, while in males it increased after FB exposure (60 mg/kg b.w.), with a simultaneous decrease in expression of the ghrelin receptor. Leptin and its receptor immunoreaction intensity was decreased in both sexes following FB exposure. The current study highlighted the potential involvement of intestinal ghrelin and leptin in the metabolic disturbances observed later in life in offspring that were prenatally exposed to fumonisins.https://www.mdpi.com/2076-2615/13/9/1538fumonisinsintestineduodenal morphologyprenatal exposure |
| spellingShingle | Ewa Tomaszewska Halyna Rudyk Piotr Dobrowolski Marcin B. Arciszewski Janine Donaldson Katarzyna Kras Beata Abramowicz Damian Kuc Siemowit Muszyński Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to Fumonisins Animals fumonisins intestine duodenal morphology prenatal exposure |
| title | Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to Fumonisins |
| title_full | Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to Fumonisins |
| title_fullStr | Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to Fumonisins |
| title_full_unstemmed | Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to Fumonisins |
| title_short | Basal Intestinal Morphology, Immunolocalization of Leptin and Ghrelin and Their Receptors in Newborn Wistar Rats after Prenatal Exposure to Fumonisins |
| title_sort | basal intestinal morphology immunolocalization of leptin and ghrelin and their receptors in newborn wistar rats after prenatal exposure to fumonisins |
| topic | fumonisins intestine duodenal morphology prenatal exposure |
| url | https://www.mdpi.com/2076-2615/13/9/1538 |
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