The Effect of Season on the Metabolic Profile of the European Clam Ruditapes decussatus as Studied by 1H-NMR Spectroscopy
In this study, the metabolome of Ruditapes decussatus, an economically and ecologically important marine bivalve species widely distributed in the Mediterranean region, was characterized by using proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy. Significant seasonal variations in the content...
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MDPI AG
2017-07-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/7/3/36 |
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| author | Violetta Aru Søren Balling Engelsen Francesco Savorani Jacopo Culurgioni Giorgia Sarais Giulia Atzori Serenella Cabiddu Flaminia Cesare Marincola |
| author_facet | Violetta Aru Søren Balling Engelsen Francesco Savorani Jacopo Culurgioni Giorgia Sarais Giulia Atzori Serenella Cabiddu Flaminia Cesare Marincola |
| author_sort | Violetta Aru |
| collection | DOAJ |
| description | In this study, the metabolome of Ruditapes decussatus, an economically and ecologically important marine bivalve species widely distributed in the Mediterranean region, was characterized by using proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy. Significant seasonal variations in the content of carbohydrates and free amino acids were observed. The relative amounts of alanine and glycine were found to exhibit the same seasonal pattern as the temperature and salinity at the harvesting site. Several putative sex-specific biomarkers were also discovered. Substantial differences were found for alanine and glycine, whose relative amounts were higher in males, while acetoacetate, choline and phosphocholine were more abundant in female clams. These findings reveal novel insights into the baseline metabolism of the European clam and represent a step forward towards a comprehensive metabolic characterization of the species. Besides providing a holistic view on the prominent nutritional components, the characterization of the metabolome of this bivalve represents an important prerequisite for elucidating the underlying metabolic pathways behind the environment-organism interactions. |
| first_indexed | 2024-12-10T03:47:23Z |
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| id | doaj.art-140a03b3deab480ea21d4e5e2dde660a |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-12-10T03:47:23Z |
| publishDate | 2017-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-140a03b3deab480ea21d4e5e2dde660a2022-12-22T02:03:21ZengMDPI AGMetabolites2218-19892017-07-01733610.3390/metabo7030036metabo7030036The Effect of Season on the Metabolic Profile of the European Clam Ruditapes decussatus as Studied by 1H-NMR SpectroscopyVioletta Aru0Søren Balling Engelsen1Francesco Savorani2Jacopo Culurgioni3Giorgia Sarais4Giulia Atzori5Serenella Cabiddu6Flaminia Cesare Marincola7Chemometrics and Analytical Technology, Department of Food Science, University of Copenhagen, 1165 København, DenmarkChemometrics and Analytical Technology, Department of Food Science, University of Copenhagen, 1165 København, DenmarkChemometrics and Analytical Technology, Department of Food Science, University of Copenhagen, 1165 København, DenmarkDepartment of Life and Environmental Sciences, University of Cagliari, 09126 Cagliari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, 09126 Cagliari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, 09126 Cagliari, ItalyDepartment of Life and Environmental Sciences, University of Cagliari, 09126 Cagliari, ItalyDepartment of Chemical and Geological Sciences, Cittadella Universitaria di Monserrato, 09042 Monserrato, ItalyIn this study, the metabolome of Ruditapes decussatus, an economically and ecologically important marine bivalve species widely distributed in the Mediterranean region, was characterized by using proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy. Significant seasonal variations in the content of carbohydrates and free amino acids were observed. The relative amounts of alanine and glycine were found to exhibit the same seasonal pattern as the temperature and salinity at the harvesting site. Several putative sex-specific biomarkers were also discovered. Substantial differences were found for alanine and glycine, whose relative amounts were higher in males, while acetoacetate, choline and phosphocholine were more abundant in female clams. These findings reveal novel insights into the baseline metabolism of the European clam and represent a step forward towards a comprehensive metabolic characterization of the species. Besides providing a holistic view on the prominent nutritional components, the characterization of the metabolome of this bivalve represents an important prerequisite for elucidating the underlying metabolic pathways behind the environment-organism interactions.https://www.mdpi.com/2218-1989/7/3/36clamsmetabolitesNMR spectroscopyRuditapes decussatusSanta Gilla lagoonseasonal change |
| spellingShingle | Violetta Aru Søren Balling Engelsen Francesco Savorani Jacopo Culurgioni Giorgia Sarais Giulia Atzori Serenella Cabiddu Flaminia Cesare Marincola The Effect of Season on the Metabolic Profile of the European Clam Ruditapes decussatus as Studied by 1H-NMR Spectroscopy Metabolites clams metabolites NMR spectroscopy Ruditapes decussatus Santa Gilla lagoon seasonal change |
| title | The Effect of Season on the Metabolic Profile of the European Clam Ruditapes decussatus as Studied by 1H-NMR Spectroscopy |
| title_full | The Effect of Season on the Metabolic Profile of the European Clam Ruditapes decussatus as Studied by 1H-NMR Spectroscopy |
| title_fullStr | The Effect of Season on the Metabolic Profile of the European Clam Ruditapes decussatus as Studied by 1H-NMR Spectroscopy |
| title_full_unstemmed | The Effect of Season on the Metabolic Profile of the European Clam Ruditapes decussatus as Studied by 1H-NMR Spectroscopy |
| title_short | The Effect of Season on the Metabolic Profile of the European Clam Ruditapes decussatus as Studied by 1H-NMR Spectroscopy |
| title_sort | effect of season on the metabolic profile of the european clam ruditapes decussatus as studied by 1h nmr spectroscopy |
| topic | clams metabolites NMR spectroscopy Ruditapes decussatus Santa Gilla lagoon seasonal change |
| url | https://www.mdpi.com/2218-1989/7/3/36 |
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