Data on preparation and characterization of an insect odorant receptor based biosensor

Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial l...

Full description

Bibliographic Details
Main Authors: Roshan Khadka, Nihan Aydemir, Colm Carraher, Cyril Hamiaux, Damon Colbert, Jamal Cheema, Jenny Malmström, Andrew Kralicek, Jadranka Travas-Sejdic
Format: Article
Language:English
Published: Elsevier 2018-12-01
Series:Data in Brief
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340918314112
_version_ 1828287613509828608
author Roshan Khadka
Nihan Aydemir
Colm Carraher
Cyril Hamiaux
Damon Colbert
Jamal Cheema
Jenny Malmström
Andrew Kralicek
Jadranka Travas-Sejdic
author_facet Roshan Khadka
Nihan Aydemir
Colm Carraher
Cyril Hamiaux
Damon Colbert
Jamal Cheema
Jenny Malmström
Andrew Kralicek
Jadranka Travas-Sejdic
author_sort Roshan Khadka
collection DOAJ
description Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial lipid bilayers (liposomes). These OrX/liposomes were covalently attached to a gold surface and characterized using quartz crystal microbalance with dissipation monitoring (QCM-D). The interaction of OrX/liposomes immobilized on a gold surface to positive and negative odorants were studied by means of electrochemical impedance spectroscopy (EIS) and QCM-D. The data presented in this article are related to the research article titled “An ultrasensitive electrochemical impedance-based biosensor using insect odorant receptors to detect odorants” [1].
first_indexed 2024-04-13T09:51:36Z
format Article
id doaj.art-140c2ec1c9b1408fb210e4625f9a5a6b
institution Directory Open Access Journal
issn 2352-3409
language English
last_indexed 2024-04-13T09:51:36Z
publishDate 2018-12-01
publisher Elsevier
record_format Article
series Data in Brief
spelling doaj.art-140c2ec1c9b1408fb210e4625f9a5a6b2022-12-22T02:51:34ZengElsevierData in Brief2352-34092018-12-012121422148Data on preparation and characterization of an insect odorant receptor based biosensorRoshan Khadka0Nihan Aydemir1Colm Carraher2Cyril Hamiaux3Damon Colbert4Jamal Cheema5Jenny Malmström6Andrew Kralicek7Jadranka Travas-Sejdic8Polymer Electronic Research Centre, School of Chemical Sciences, University of Auckland, Auckland 1023, New Zealand; MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New ZealandPolymer Electronic Research Centre, School of Chemical Sciences, University of Auckland, Auckland 1023, New Zealand; MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland 1142, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland 1142, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland 1142, New ZealandPolymer Electronic Research Centre, School of Chemical Sciences, University of Auckland, Auckland 1023, New Zealand; MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New ZealandMacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New Zealand; Department of Chemical and Materials Engineering, University of Auckland, Auckland 1023, New ZealandThe New Zealand Institute for Plant and Food Research Limited, Private Bag 92169, Auckland 1142, New Zealand; Corresponding authors.Polymer Electronic Research Centre, School of Chemical Sciences, University of Auckland, Auckland 1023, New Zealand; MacDiarmid Institute for Advanced Materials and Nanotechnology, Wellington 6140, New Zealand; Corresponding authors.Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial lipid bilayers (liposomes). These OrX/liposomes were covalently attached to a gold surface and characterized using quartz crystal microbalance with dissipation monitoring (QCM-D). The interaction of OrX/liposomes immobilized on a gold surface to positive and negative odorants were studied by means of electrochemical impedance spectroscopy (EIS) and QCM-D. The data presented in this article are related to the research article titled “An ultrasensitive electrochemical impedance-based biosensor using insect odorant receptors to detect odorants” [1].http://www.sciencedirect.com/science/article/pii/S2352340918314112
spellingShingle Roshan Khadka
Nihan Aydemir
Colm Carraher
Cyril Hamiaux
Damon Colbert
Jamal Cheema
Jenny Malmström
Andrew Kralicek
Jadranka Travas-Sejdic
Data on preparation and characterization of an insect odorant receptor based biosensor
Data in Brief
title Data on preparation and characterization of an insect odorant receptor based biosensor
title_full Data on preparation and characterization of an insect odorant receptor based biosensor
title_fullStr Data on preparation and characterization of an insect odorant receptor based biosensor
title_full_unstemmed Data on preparation and characterization of an insect odorant receptor based biosensor
title_short Data on preparation and characterization of an insect odorant receptor based biosensor
title_sort data on preparation and characterization of an insect odorant receptor based biosensor
url http://www.sciencedirect.com/science/article/pii/S2352340918314112
work_keys_str_mv AT roshankhadka dataonpreparationandcharacterizationofaninsectodorantreceptorbasedbiosensor
AT nihanaydemir dataonpreparationandcharacterizationofaninsectodorantreceptorbasedbiosensor
AT colmcarraher dataonpreparationandcharacterizationofaninsectodorantreceptorbasedbiosensor
AT cyrilhamiaux dataonpreparationandcharacterizationofaninsectodorantreceptorbasedbiosensor
AT damoncolbert dataonpreparationandcharacterizationofaninsectodorantreceptorbasedbiosensor
AT jamalcheema dataonpreparationandcharacterizationofaninsectodorantreceptorbasedbiosensor
AT jennymalmstrom dataonpreparationandcharacterizationofaninsectodorantreceptorbasedbiosensor
AT andrewkralicek dataonpreparationandcharacterizationofaninsectodorantreceptorbasedbiosensor
AT jadrankatravassejdic dataonpreparationandcharacterizationofaninsectodorantreceptorbasedbiosensor