Bone marrow mesenchymal cell adhesion to polished and nitrided titanium surfaces

Aim: To evaluate the adhesion of mouse bone marrow mesenchymal cells (MBMMC) on different titanium surfaces. Methods: Grade II titanium discs (ASTM F86) received two different surface treatments: polished (S1) and cathodic cage plasma nitriding (S2). MBMMC were cultured on titanium discs in 24-well...

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Main Authors: Luciana Bastos Alves, Fernanda Ginani, José Sandro Pereira da Silva, Carlos Augusto Galvão Barboza, Clodomiro Alves Junior
Format: Article
Language:English
Published: Universidade Estadual de Campinas 2015-11-01
Series:Brazilian Journal of Oral Sciences
Subjects:
Online Access:https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641611
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author Luciana Bastos Alves
Fernanda Ginani
José Sandro Pereira da Silva
Carlos Augusto Galvão Barboza
Clodomiro Alves Junior
author_facet Luciana Bastos Alves
Fernanda Ginani
José Sandro Pereira da Silva
Carlos Augusto Galvão Barboza
Clodomiro Alves Junior
author_sort Luciana Bastos Alves
collection DOAJ
description Aim: To evaluate the adhesion of mouse bone marrow mesenchymal cells (MBMMC) on different titanium surfaces. Methods: Grade II titanium discs (ASTM F86) received two different surface treatments: polished (S1) and cathodic cage plasma nitriding (S2). MBMMC were cultured on titanium discs in 24-well cell culture plates, at a density of 1 x 104 cells per well. After 24 h, the adhesion was evaluated using a hemocytometer. Results: The mean adhesion was greater on S2, though without statistically significant difference from S2 (p>0.05). Conclusions: It was demonstrated that titanium surface treatment with ionic nitriding in a cathodic cage is biocompatible since it preserved the integrity of the cultivated MBMMC for a period of 24 h, allowing their adhesion.
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spelling doaj.art-140cafe2e5b549829a23de1101d6b2582022-12-21T20:28:23ZengUniversidade Estadual de CampinasBrazilian Journal of Oral Sciences1677-32252015-11-0110410.20396/bjos.v10i4.8641611Bone marrow mesenchymal cell adhesion to polished and nitrided titanium surfacesLuciana Bastos Alves0Fernanda Ginani1José Sandro Pereira da Silva2Carlos Augusto Galvão Barboza3Clodomiro Alves Junior4Federal University of Rio Grande do NorteFederal University of Rio Grande do NorteFederal University of Rio Grande do NorteFederal University of Rio Grande do NorteFederal University of Rio Grande do NorteAim: To evaluate the adhesion of mouse bone marrow mesenchymal cells (MBMMC) on different titanium surfaces. Methods: Grade II titanium discs (ASTM F86) received two different surface treatments: polished (S1) and cathodic cage plasma nitriding (S2). MBMMC were cultured on titanium discs in 24-well cell culture plates, at a density of 1 x 104 cells per well. After 24 h, the adhesion was evaluated using a hemocytometer. Results: The mean adhesion was greater on S2, though without statistically significant difference from S2 (p>0.05). Conclusions: It was demonstrated that titanium surface treatment with ionic nitriding in a cathodic cage is biocompatible since it preserved the integrity of the cultivated MBMMC for a period of 24 h, allowing their adhesion.https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641611Mesenchymal stem cellsBone marrowTitanium
spellingShingle Luciana Bastos Alves
Fernanda Ginani
José Sandro Pereira da Silva
Carlos Augusto Galvão Barboza
Clodomiro Alves Junior
Bone marrow mesenchymal cell adhesion to polished and nitrided titanium surfaces
Brazilian Journal of Oral Sciences
Mesenchymal stem cells
Bone marrow
Titanium
title Bone marrow mesenchymal cell adhesion to polished and nitrided titanium surfaces
title_full Bone marrow mesenchymal cell adhesion to polished and nitrided titanium surfaces
title_fullStr Bone marrow mesenchymal cell adhesion to polished and nitrided titanium surfaces
title_full_unstemmed Bone marrow mesenchymal cell adhesion to polished and nitrided titanium surfaces
title_short Bone marrow mesenchymal cell adhesion to polished and nitrided titanium surfaces
title_sort bone marrow mesenchymal cell adhesion to polished and nitrided titanium surfaces
topic Mesenchymal stem cells
Bone marrow
Titanium
url https://periodicos.sbu.unicamp.br/ojs/index.php/bjos/article/view/8641611
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AT carlosaugustogalvaobarboza bonemarrowmesenchymalcelladhesiontopolishedandnitridedtitaniumsurfaces
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