From nanoparticles to crystals: one-pot programmable biosynthesis of photothermal gold structures and their use for biomedical applications
Abstract Inspired by nature, green chemistry uses various biomolecules, such as proteins, as reducing agents to synthesize metallic nanostructures. This methodology provides an alternative route to conventional harsh synthetic processes, which include polluting chemicals. Tuning the resulting nanost...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-11-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12951-022-01680-7 |
| _version_ | 1828094111890014208 |
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| author | Roman Nudelman Hashim Alhmoud Bahman Delalat Ishdeep Kaur Anastasia Vitkin Laure Bourgeois Ilan Goldfarb Anna Cifuentes-Rius Nicolas H. Voelcker Shachar Richter |
| author_facet | Roman Nudelman Hashim Alhmoud Bahman Delalat Ishdeep Kaur Anastasia Vitkin Laure Bourgeois Ilan Goldfarb Anna Cifuentes-Rius Nicolas H. Voelcker Shachar Richter |
| author_sort | Roman Nudelman |
| collection | DOAJ |
| description | Abstract Inspired by nature, green chemistry uses various biomolecules, such as proteins, as reducing agents to synthesize metallic nanostructures. This methodology provides an alternative route to conventional harsh synthetic processes, which include polluting chemicals. Tuning the resulting nanostructure properties, such as their size and shape, is challenging as the exact mechanism involved in their formation is still not well understood. This work reports a well-controlled method to program gold nanostructures' shape, size, and aggregation state using only one protein type, mucin, as a reduction and capping material in a one-pot bio-assisted reaction. Using mucin as a gold reduction template while varying its tertiary structure via the pH of the synthesis, we demonstrate that spherical, coral-shaped, and hexagonal gold crystals can be obtained and that the size can be tuned over three orders of magnitude. This is achieved by leveraging the protein's intrinsic reducing properties and pH-induced conformational changes. The systematic study of the reaction kinetics and growth steps developed here provides an understanding of the mechanism behind this phenomenon. We further show that the prepared gold nanostructures exhibit tunable photothermal properties that can be optimized for various hyperthermia-induced antibacterial applications. |
| first_indexed | 2024-04-11T06:54:13Z |
| format | Article |
| id | doaj.art-1411e610e9eb42e5baf61757dace94ab |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-11T06:54:13Z |
| publishDate | 2022-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-1411e610e9eb42e5baf61757dace94ab2022-12-22T04:39:04ZengBMCJournal of Nanobiotechnology1477-31552022-11-0120111310.1186/s12951-022-01680-7From nanoparticles to crystals: one-pot programmable biosynthesis of photothermal gold structures and their use for biomedical applicationsRoman Nudelman0Hashim Alhmoud1Bahman Delalat2Ishdeep Kaur3Anastasia Vitkin4Laure Bourgeois5Ilan Goldfarb6Anna Cifuentes-Rius7Nicolas H. Voelcker8Shachar Richter9Department of Material Science and Engineering, Center for Nano-Science and Nano-Technology, Tel-Aviv UniversityMonash Institute of Pharmaceutical Sciences, Monash UniversityCSIRO ManufacturingMonash Institute of Pharmaceutical Sciences, Monash UniversityDepartment of Material Science and Engineering, Center for Nano-Science and Nano-Technology, Tel-Aviv UniversityMonash Centre for Electron Microscopy, Department of Materials Science and Engineering, Faculty of Engineering, Monash UniversityDepartment of Material Science and Engineering, Center for Nano-Science and Nano-Technology, Tel-Aviv UniversityMonash Institute of Pharmaceutical Sciences, Monash UniversityMonash Institute of Pharmaceutical Sciences, Monash UniversityDepartment of Material Science and Engineering, Center for Nano-Science and Nano-Technology, Tel-Aviv UniversityAbstract Inspired by nature, green chemistry uses various biomolecules, such as proteins, as reducing agents to synthesize metallic nanostructures. This methodology provides an alternative route to conventional harsh synthetic processes, which include polluting chemicals. Tuning the resulting nanostructure properties, such as their size and shape, is challenging as the exact mechanism involved in their formation is still not well understood. This work reports a well-controlled method to program gold nanostructures' shape, size, and aggregation state using only one protein type, mucin, as a reduction and capping material in a one-pot bio-assisted reaction. Using mucin as a gold reduction template while varying its tertiary structure via the pH of the synthesis, we demonstrate that spherical, coral-shaped, and hexagonal gold crystals can be obtained and that the size can be tuned over three orders of magnitude. This is achieved by leveraging the protein's intrinsic reducing properties and pH-induced conformational changes. The systematic study of the reaction kinetics and growth steps developed here provides an understanding of the mechanism behind this phenomenon. We further show that the prepared gold nanostructures exhibit tunable photothermal properties that can be optimized for various hyperthermia-induced antibacterial applications.https://doi.org/10.1186/s12951-022-01680-7Protein-templated synthesisGold nanoparticlesMucinGreen synthesisPhotothermal MaterialsAntibacterial activity |
| spellingShingle | Roman Nudelman Hashim Alhmoud Bahman Delalat Ishdeep Kaur Anastasia Vitkin Laure Bourgeois Ilan Goldfarb Anna Cifuentes-Rius Nicolas H. Voelcker Shachar Richter From nanoparticles to crystals: one-pot programmable biosynthesis of photothermal gold structures and their use for biomedical applications Journal of Nanobiotechnology Protein-templated synthesis Gold nanoparticles Mucin Green synthesis Photothermal Materials Antibacterial activity |
| title | From nanoparticles to crystals: one-pot programmable biosynthesis of photothermal gold structures and their use for biomedical applications |
| title_full | From nanoparticles to crystals: one-pot programmable biosynthesis of photothermal gold structures and their use for biomedical applications |
| title_fullStr | From nanoparticles to crystals: one-pot programmable biosynthesis of photothermal gold structures and their use for biomedical applications |
| title_full_unstemmed | From nanoparticles to crystals: one-pot programmable biosynthesis of photothermal gold structures and their use for biomedical applications |
| title_short | From nanoparticles to crystals: one-pot programmable biosynthesis of photothermal gold structures and their use for biomedical applications |
| title_sort | from nanoparticles to crystals one pot programmable biosynthesis of photothermal gold structures and their use for biomedical applications |
| topic | Protein-templated synthesis Gold nanoparticles Mucin Green synthesis Photothermal Materials Antibacterial activity |
| url | https://doi.org/10.1186/s12951-022-01680-7 |
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