Comparative Evaluation of Solubility, Cytotoxicity and Photostability Studies of Resveratrol and Oxyresveratrol Loaded Nanosponges
Resveratrol and oxyresveratrol are natural polyphenolic stilbenes with several important pharmacological activities. However, low solubility and aqueous instability are the major limitations in their drug delivery applications. In the present work, we demonstrated the encapsulation of resveratrol an...
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| Format: | Article |
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MDPI AG
2019-10-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/11/10/545 |
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| author | Nilesh Kumar Dhakar Adrián Matencio Fabrizio Caldera Monica Argenziano Roberta Cavalli Chiara Dianzani Marco Zanetti José Manuel López-Nicolás Francesco Trotta |
| author_facet | Nilesh Kumar Dhakar Adrián Matencio Fabrizio Caldera Monica Argenziano Roberta Cavalli Chiara Dianzani Marco Zanetti José Manuel López-Nicolás Francesco Trotta |
| author_sort | Nilesh Kumar Dhakar |
| collection | DOAJ |
| description | Resveratrol and oxyresveratrol are natural polyphenolic stilbenes with several important pharmacological activities. However, low solubility and aqueous instability are the major limitations in their drug delivery applications. In the present work, we demonstrated the encapsulation of resveratrol and oxyresveratrol with nanosponge to improve solubility and stability. Several characterization techniques were used to confirm the encapsulation of both drug molecules within the nanosponges. The high encapsulation efficiency of resveratrol (77.73%) and oxyresveratrol (80.33%) was achieved within the nanosponges. Transmission electron microscopy suggested uniform spherical size particles of resveratrol and oxyresveratrol loaded nanosponges. Compared to free drugs, better protection against UV degradation was observed for resveratrol-loaded nanosponge (2-fold) and oxyresveratrol-loaded nanosponge (3-fold). Moreover, a higher solubilization of resveratrol- and oxyresveratrol-loaded nanosponges lead to a better antioxidant activity compared to drug molecules alone. Cytotoxicity studies against DU-145 prostate cancer cell lines further suggested improved activity of both resveratrol and oxyresveratrol-loaded nanosponges without any significant toxicity of blank nanosponges. |
| first_indexed | 2024-12-10T07:02:06Z |
| format | Article |
| id | doaj.art-14162655214141b68f9e3a22430262d5 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-12-10T07:02:06Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-14162655214141b68f9e3a22430262d52022-12-22T01:58:18ZengMDPI AGPharmaceutics1999-49232019-10-01111054510.3390/pharmaceutics11100545pharmaceutics11100545Comparative Evaluation of Solubility, Cytotoxicity and Photostability Studies of Resveratrol and Oxyresveratrol Loaded NanospongesNilesh Kumar Dhakar0Adrián Matencio1Fabrizio Caldera2Monica Argenziano3Roberta Cavalli4Chiara Dianzani5Marco Zanetti6José Manuel López-Nicolás7Francesco Trotta8Department of Chemistry, University of Torino, via P. Giuria 7, 10125 Torino, ItalyDepartment of Biochemistry and Molecular Biology-A, Faculty of Biology, 13 University of Murcia - Regional Campus of International Excellence “Campus Mare 14 Nostrum”, E-30100 Murcia, SpainDepartment of Chemistry, University of Torino, via P. Giuria 7, 10125 Torino, ItalyDepartment of Drug Science and Technology, University of Torino, via P. Giuria 9, 10125 Torino, ItalyDepartment of Drug Science and Technology, University of Torino, via P. Giuria 9, 10125 Torino, ItalyDepartment of Drug Science and Technology, University of Torino, via P. Giuria 9, 10125 Torino, ItalyDepartment of Chemistry, University of Torino, via P. Giuria 7, 10125 Torino, ItalyDepartment of Biochemistry and Molecular Biology-A, Faculty of Biology, 13 University of Murcia - Regional Campus of International Excellence “Campus Mare 14 Nostrum”, E-30100 Murcia, SpainDepartment of Chemistry, University of Torino, via P. Giuria 7, 10125 Torino, ItalyResveratrol and oxyresveratrol are natural polyphenolic stilbenes with several important pharmacological activities. However, low solubility and aqueous instability are the major limitations in their drug delivery applications. In the present work, we demonstrated the encapsulation of resveratrol and oxyresveratrol with nanosponge to improve solubility and stability. Several characterization techniques were used to confirm the encapsulation of both drug molecules within the nanosponges. The high encapsulation efficiency of resveratrol (77.73%) and oxyresveratrol (80.33%) was achieved within the nanosponges. Transmission electron microscopy suggested uniform spherical size particles of resveratrol and oxyresveratrol loaded nanosponges. Compared to free drugs, better protection against UV degradation was observed for resveratrol-loaded nanosponge (2-fold) and oxyresveratrol-loaded nanosponge (3-fold). Moreover, a higher solubilization of resveratrol- and oxyresveratrol-loaded nanosponges lead to a better antioxidant activity compared to drug molecules alone. Cytotoxicity studies against DU-145 prostate cancer cell lines further suggested improved activity of both resveratrol and oxyresveratrol-loaded nanosponges without any significant toxicity of blank nanosponges.https://www.mdpi.com/1999-4923/11/10/545resveratroloxyresveratrolβ-cyclodextrinnanospongessolubility enhancementdrug-deliverycytotoxicitystability studies |
| spellingShingle | Nilesh Kumar Dhakar Adrián Matencio Fabrizio Caldera Monica Argenziano Roberta Cavalli Chiara Dianzani Marco Zanetti José Manuel López-Nicolás Francesco Trotta Comparative Evaluation of Solubility, Cytotoxicity and Photostability Studies of Resveratrol and Oxyresveratrol Loaded Nanosponges Pharmaceutics resveratrol oxyresveratrol β-cyclodextrin nanosponges solubility enhancement drug-delivery cytotoxicity stability studies |
| title | Comparative Evaluation of Solubility, Cytotoxicity and Photostability Studies of Resveratrol and Oxyresveratrol Loaded Nanosponges |
| title_full | Comparative Evaluation of Solubility, Cytotoxicity and Photostability Studies of Resveratrol and Oxyresveratrol Loaded Nanosponges |
| title_fullStr | Comparative Evaluation of Solubility, Cytotoxicity and Photostability Studies of Resveratrol and Oxyresveratrol Loaded Nanosponges |
| title_full_unstemmed | Comparative Evaluation of Solubility, Cytotoxicity and Photostability Studies of Resveratrol and Oxyresveratrol Loaded Nanosponges |
| title_short | Comparative Evaluation of Solubility, Cytotoxicity and Photostability Studies of Resveratrol and Oxyresveratrol Loaded Nanosponges |
| title_sort | comparative evaluation of solubility cytotoxicity and photostability studies of resveratrol and oxyresveratrol loaded nanosponges |
| topic | resveratrol oxyresveratrol β-cyclodextrin nanosponges solubility enhancement drug-delivery cytotoxicity stability studies |
| url | https://www.mdpi.com/1999-4923/11/10/545 |
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