Biotransformation of Androstenedione by Filamentous Fungi Isolated from Cultural Heritage Sites in the State Tretyakov Gallery
The transformation of steroids by microorganisms is widely used in medical biotechnology. A huge group of filamentous fungi is one of the most promising taxa for screening new biocatalytic reactions in order to obtain pharmaceutically significant steroids. In this work, we screened 10 filamentous fu...
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MDPI AG
2022-06-01
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| author | Alexander A. Zhgun Mark P. Potapov Darya A. Avdanina Natalya V. Karpova Vera V. Yaderets Vakhtang V. Dzhavakhiya Dmitry A. Kardonsky |
| author_facet | Alexander A. Zhgun Mark P. Potapov Darya A. Avdanina Natalya V. Karpova Vera V. Yaderets Vakhtang V. Dzhavakhiya Dmitry A. Kardonsky |
| author_sort | Alexander A. Zhgun |
| collection | DOAJ |
| description | The transformation of steroids by microorganisms is widely used in medical biotechnology. A huge group of filamentous fungi is one of the most promising taxa for screening new biocatalytic reactions in order to obtain pharmaceutically significant steroids. In this work, we screened 10 filamentous fungi-destructors of egg tempera for the ability to biotransform androst-4-en-3,17-dione (AD) during cultivation in a liquid nutrient medium or in a buffer solution. These taxonomically unrelated strains, belonging to the classes <i>Eurotiomycetes</i>, <i>Dothideomycetes</i> and <i>Sordariomycetes</i>, are dominant representatives of the microbiome from halls where works of tempera painting are stored in the State Tretyakov Gallery (STG, Moscow, Russia). Since the binder of tempera paints, egg yolk, contains about 2% cholesterol, these degrading fungi appear to be a promising group for screening for steroid converting activity. It turned out that all the studied fungi-destructors are able to transform AD. Some strains showed transformation efficiency close to the industrial strain <i>Curvularia lunata</i> RNCIM F-981. In total, 33 steroids formed during the transformation of AD were characterized, for 19 of them the structure was established by gas chromatography/mass spectrometry analysis. In this work, we have shown for the first time that fungi-destructors of tempera paintings can efficiently transform steroids. |
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| id | doaj.art-141a0aef46a1435080e0cc506373f85f |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T00:22:27Z |
| publishDate | 2022-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-141a0aef46a1435080e0cc506373f85f2023-11-23T15:39:54ZengMDPI AGBiology2079-77372022-06-0111688310.3390/biology11060883Biotransformation of Androstenedione by Filamentous Fungi Isolated from Cultural Heritage Sites in the State Tretyakov GalleryAlexander A. Zhgun0Mark P. Potapov1Darya A. Avdanina2Natalya V. Karpova3Vera V. Yaderets4Vakhtang V. Dzhavakhiya5Dmitry A. Kardonsky6Research Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, RussiaResearch Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, RussiaResearch Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, RussiaResearch Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, RussiaResearch Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, RussiaResearch Center of Biotechnology, Russian Academy of Sciences, 119071 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, 119435 Moscow, RussiaThe transformation of steroids by microorganisms is widely used in medical biotechnology. A huge group of filamentous fungi is one of the most promising taxa for screening new biocatalytic reactions in order to obtain pharmaceutically significant steroids. In this work, we screened 10 filamentous fungi-destructors of egg tempera for the ability to biotransform androst-4-en-3,17-dione (AD) during cultivation in a liquid nutrient medium or in a buffer solution. These taxonomically unrelated strains, belonging to the classes <i>Eurotiomycetes</i>, <i>Dothideomycetes</i> and <i>Sordariomycetes</i>, are dominant representatives of the microbiome from halls where works of tempera painting are stored in the State Tretyakov Gallery (STG, Moscow, Russia). Since the binder of tempera paints, egg yolk, contains about 2% cholesterol, these degrading fungi appear to be a promising group for screening for steroid converting activity. It turned out that all the studied fungi-destructors are able to transform AD. Some strains showed transformation efficiency close to the industrial strain <i>Curvularia lunata</i> RNCIM F-981. In total, 33 steroids formed during the transformation of AD were characterized, for 19 of them the structure was established by gas chromatography/mass spectrometry analysis. In this work, we have shown for the first time that fungi-destructors of tempera paintings can efficiently transform steroids.https://www.mdpi.com/2079-7737/11/6/883steroid biotransformationfilamentous fungibiodeterioration of tempera paintingcholesterolandrost-4-en-3,17-dione |
| spellingShingle | Alexander A. Zhgun Mark P. Potapov Darya A. Avdanina Natalya V. Karpova Vera V. Yaderets Vakhtang V. Dzhavakhiya Dmitry A. Kardonsky Biotransformation of Androstenedione by Filamentous Fungi Isolated from Cultural Heritage Sites in the State Tretyakov Gallery Biology steroid biotransformation filamentous fungi biodeterioration of tempera painting cholesterol androst-4-en-3,17-dione |
| title | Biotransformation of Androstenedione by Filamentous Fungi Isolated from Cultural Heritage Sites in the State Tretyakov Gallery |
| title_full | Biotransformation of Androstenedione by Filamentous Fungi Isolated from Cultural Heritage Sites in the State Tretyakov Gallery |
| title_fullStr | Biotransformation of Androstenedione by Filamentous Fungi Isolated from Cultural Heritage Sites in the State Tretyakov Gallery |
| title_full_unstemmed | Biotransformation of Androstenedione by Filamentous Fungi Isolated from Cultural Heritage Sites in the State Tretyakov Gallery |
| title_short | Biotransformation of Androstenedione by Filamentous Fungi Isolated from Cultural Heritage Sites in the State Tretyakov Gallery |
| title_sort | biotransformation of androstenedione by filamentous fungi isolated from cultural heritage sites in the state tretyakov gallery |
| topic | steroid biotransformation filamentous fungi biodeterioration of tempera painting cholesterol androst-4-en-3,17-dione |
| url | https://www.mdpi.com/2079-7737/11/6/883 |
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