Analysis of extracellular RNA by digital PCR

Analysis of extracellular RNA by digital PCR

The transfer of extracellular RNA is emerging as an important mechanism for intracellular communication. The ability for the transfer of functionally active RNA molecules from one cell to another within vesicles such as exosomes enables a cell to modulate cellular signaling and biological processes...

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Bibliographic Details
Main Authors: Kenji eTakahashi, Irene K Yan, Chaeyoung eKim, Jungsu eKim, Tushar ePatel
Format: Article
Language:English
Published: Frontiers Media S.A. 2014-06-01
Series:Frontiers in Oncology
Subjects:
Exosomes
Gene Expression
non-coding RNA
detection
Real-Time PCR
extracellular vesicles
Online Access:http://journal.frontiersin.org/Journal/10.3389/fonc.2014.00129/full
Description
Summary:The transfer of extracellular RNA is emerging as an important mechanism for intracellular communication. The ability for the transfer of functionally active RNA molecules from one cell to another within vesicles such as exosomes enables a cell to modulate cellular signaling and biological processes within recipient cells. The study of extracellular RNA requires sensitive methods for the detection of these molecules. In this methods article, we will describe protocols for the detection of such extracellular RNA using sensitive detection technologies such as digital PCR. These protocols should be valuable to researchers interested in the role and contribution of extracellular RNA to tumor cell biology.
ISSN:2234-943X

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