Analysis of extracellular RNA by digital PCR
The transfer of extracellular RNA is emerging as an important mechanism for intracellular communication. The ability for the transfer of functionally active RNA molecules from one cell to another within vesicles such as exosomes enables a cell to modulate cellular signaling and biological processes...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2014-06-01
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| Series: | Frontiers in Oncology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fonc.2014.00129/full |
| _version_ | 1817994532036280320 |
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| author | Kenji eTakahashi Irene K Yan Chaeyoung eKim Jungsu eKim Tushar ePatel |
| author_facet | Kenji eTakahashi Irene K Yan Chaeyoung eKim Jungsu eKim Tushar ePatel |
| author_sort | Kenji eTakahashi |
| collection | DOAJ |
| description | The transfer of extracellular RNA is emerging as an important mechanism for intracellular communication. The ability for the transfer of functionally active RNA molecules from one cell to another within vesicles such as exosomes enables a cell to modulate cellular signaling and biological processes within recipient cells. The study of extracellular RNA requires sensitive methods for the detection of these molecules. In this methods article, we will describe protocols for the detection of such extracellular RNA using sensitive detection technologies such as digital PCR. These protocols should be valuable to researchers interested in the role and contribution of extracellular RNA to tumor cell biology. |
| first_indexed | 2024-04-14T01:54:09Z |
| format | Article |
| id | doaj.art-141b3fdedbe344bfa59d115b2b0006a7 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-04-14T01:54:09Z |
| publishDate | 2014-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-141b3fdedbe344bfa59d115b2b0006a72022-12-22T02:19:10ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2014-06-01410.3389/fonc.2014.0012987485Analysis of extracellular RNA by digital PCRKenji eTakahashi0Irene K Yan1Chaeyoung eKim2Jungsu eKim3Tushar ePatel4Mayo ClinicMayo ClinicMayo ClinicMayo ClinicMayo ClinicThe transfer of extracellular RNA is emerging as an important mechanism for intracellular communication. The ability for the transfer of functionally active RNA molecules from one cell to another within vesicles such as exosomes enables a cell to modulate cellular signaling and biological processes within recipient cells. The study of extracellular RNA requires sensitive methods for the detection of these molecules. In this methods article, we will describe protocols for the detection of such extracellular RNA using sensitive detection technologies such as digital PCR. These protocols should be valuable to researchers interested in the role and contribution of extracellular RNA to tumor cell biology.http://journal.frontiersin.org/Journal/10.3389/fonc.2014.00129/fullExosomesGene Expressionnon-coding RNAdetectionReal-Time PCRextracellular vesicles |
| spellingShingle | Kenji eTakahashi Irene K Yan Chaeyoung eKim Jungsu eKim Tushar ePatel Analysis of extracellular RNA by digital PCR Frontiers in Oncology Exosomes Gene Expression non-coding RNA detection Real-Time PCR extracellular vesicles |
| title | Analysis of extracellular RNA by digital PCR |
| title_full | Analysis of extracellular RNA by digital PCR |
| title_fullStr | Analysis of extracellular RNA by digital PCR |
| title_full_unstemmed | Analysis of extracellular RNA by digital PCR |
| title_short | Analysis of extracellular RNA by digital PCR |
| title_sort | analysis of extracellular rna by digital pcr |
| topic | Exosomes Gene Expression non-coding RNA detection Real-Time PCR extracellular vesicles |
| url | http://journal.frontiersin.org/Journal/10.3389/fonc.2014.00129/full |
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