p16(INK4a) translation suppressed by miR-24.
Expression of the tumor suppressor p16(INK4a) increases during aging and replicative senescence.Here, we report that the microRNA miR-24 suppresses p16 expression in human diploid fibroblasts and cervical carcinoma cells. Increased p16 expression with replicative senescence was associated with decre...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-03-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2274865?pdf=render |
| _version_ | 1817979812176723968 |
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| author | Ashish Lal Hyeon Ho Kim Kotb Abdelmohsen Yuki Kuwano Rudolf Pullmann Subramanya Srikantan Ramesh Subrahmanyam Jennifer L Martindale Xiaoling Yang Fariyal Ahmed Francisco Navarro Derek Dykxhoorn Judy Lieberman Myriam Gorospe |
| author_facet | Ashish Lal Hyeon Ho Kim Kotb Abdelmohsen Yuki Kuwano Rudolf Pullmann Subramanya Srikantan Ramesh Subrahmanyam Jennifer L Martindale Xiaoling Yang Fariyal Ahmed Francisco Navarro Derek Dykxhoorn Judy Lieberman Myriam Gorospe |
| author_sort | Ashish Lal |
| collection | DOAJ |
| description | Expression of the tumor suppressor p16(INK4a) increases during aging and replicative senescence.Here, we report that the microRNA miR-24 suppresses p16 expression in human diploid fibroblasts and cervical carcinoma cells. Increased p16 expression with replicative senescence was associated with decreased levels of miR-24, a microRNA that was predicted to associate with the p16 mRNA coding and 3'-untranslated regions. Ectopic miR-24 overexpression reduced p16 protein but not p16 mRNA levels. Conversely, introduction of antisense (AS)-miR-24 blocked miR-24 expression and markedly enhanced p16 protein levels, p16 translation, and the production of EGFP-p16 reporter bearing the miR-24 target recognition sites.Together, our results suggest that miR-24 represses the initiation and elongation phases of p16 translation. |
| first_indexed | 2024-04-13T22:47:10Z |
| format | Article |
| id | doaj.art-141f3df44cd24439bdc4dd836d1595e1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-13T22:47:10Z |
| publishDate | 2008-03-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-141f3df44cd24439bdc4dd836d1595e12022-12-22T02:26:21ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-03-0133e186410.1371/journal.pone.0001864p16(INK4a) translation suppressed by miR-24.Ashish LalHyeon Ho KimKotb AbdelmohsenYuki KuwanoRudolf PullmannSubramanya SrikantanRamesh SubrahmanyamJennifer L MartindaleXiaoling YangFariyal AhmedFrancisco NavarroDerek DykxhoornJudy LiebermanMyriam GorospeExpression of the tumor suppressor p16(INK4a) increases during aging and replicative senescence.Here, we report that the microRNA miR-24 suppresses p16 expression in human diploid fibroblasts and cervical carcinoma cells. Increased p16 expression with replicative senescence was associated with decreased levels of miR-24, a microRNA that was predicted to associate with the p16 mRNA coding and 3'-untranslated regions. Ectopic miR-24 overexpression reduced p16 protein but not p16 mRNA levels. Conversely, introduction of antisense (AS)-miR-24 blocked miR-24 expression and markedly enhanced p16 protein levels, p16 translation, and the production of EGFP-p16 reporter bearing the miR-24 target recognition sites.Together, our results suggest that miR-24 represses the initiation and elongation phases of p16 translation.http://europepmc.org/articles/PMC2274865?pdf=render |
| spellingShingle | Ashish Lal Hyeon Ho Kim Kotb Abdelmohsen Yuki Kuwano Rudolf Pullmann Subramanya Srikantan Ramesh Subrahmanyam Jennifer L Martindale Xiaoling Yang Fariyal Ahmed Francisco Navarro Derek Dykxhoorn Judy Lieberman Myriam Gorospe p16(INK4a) translation suppressed by miR-24. PLoS ONE |
| title | p16(INK4a) translation suppressed by miR-24. |
| title_full | p16(INK4a) translation suppressed by miR-24. |
| title_fullStr | p16(INK4a) translation suppressed by miR-24. |
| title_full_unstemmed | p16(INK4a) translation suppressed by miR-24. |
| title_short | p16(INK4a) translation suppressed by miR-24. |
| title_sort | p16 ink4a translation suppressed by mir 24 |
| url | http://europepmc.org/articles/PMC2274865?pdf=render |
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