Phenotypic and genotypic characterization of Colombian clinical isolates of Sporothrix spp.
Introduction. For over a century, Sporothrix schenckii was considered the sole species responsible for sporotrichosis. In 2007, scientific community confirmed the disease could be caused by various Sporothrix species. These species differed in their virulence factors and their antifungal sensitivity...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Instituto Nacional de Salud
2023-08-01
|
| Series: | Biomédica: revista del Instituto Nacional de Salud |
| Subjects: | |
| Online Access: | https://revistabiomedica.org/index.php/biomedica/article/view/6898 |
| _version_ | 1797682108820357120 |
|---|---|
| author | Laura C. Álvarez-Acevedo María C. Zuleta-González Óscar M. Gómez-Guzmán Álvaro L. Rúa-Giraldo Orville Hernández-Ruiz Juan G. McEwen-Ochoa Martha E. Urán-Jiménez Myrtha Arango-Arteaga Rosely M. Zancopé-Oliveira Manoel Marques Evangelista de Oliveira María del P. Jiménez-Alzate |
| author_facet | Laura C. Álvarez-Acevedo María C. Zuleta-González Óscar M. Gómez-Guzmán Álvaro L. Rúa-Giraldo Orville Hernández-Ruiz Juan G. McEwen-Ochoa Martha E. Urán-Jiménez Myrtha Arango-Arteaga Rosely M. Zancopé-Oliveira Manoel Marques Evangelista de Oliveira María del P. Jiménez-Alzate |
| author_sort | Laura C. Álvarez-Acevedo |
| collection | DOAJ |
| description | Introduction. For over a century, Sporothrix schenckii was considered the sole species responsible for sporotrichosis. In 2007, scientific community confirmed the disease could be caused by various Sporothrix species. These species differed in their virulence factors and their antifungal sensitivity.
Objective. This study aims to characterize 42 Colombian clinical isolates of Sporothrix spp. phenotypically and genotypically.
Material and methods. Forty-two clinical isolates were characterized using phenotypic methods. It involved various culture media to determine their growth range at different temperatures and to assess the type and distribution of pigment and colony texture. Microscopic morphology was evaluated through microcultures, as well as the conidia diameter, type of sporulation, and morphology. Additionally, the assimilation of carbohydrates was selected as a physiological trait for species identification. Genotyping of 40 isolates was performed through partial amplification of the calmodulin gene, followed by sequence analysis.
Results. Molecular studies enabled the identification of 32 isolates of S. schenckii and 8 isolates of S. globosa. The combination of phenotypic and genotypic methods eased these species characterizations and the recognition keys development based on parameters such as growth diameter at 25 and 30 ºC, colony texture (membranous or velvety) on potato dextrose agar, and microscopic morphology with predominance of pigmented triangular, elongated oval globose, or subglobose conidia.
Conclusions. Confirmation of the phenotypic characteristics and molecular analysis is crucial for identifying Sporothrix species and determining adequate treatment. This study represents the first phenotypical and genotypical characterization of clinical isolates of Sporothrix spp. reported in Colombia. |
| first_indexed | 2024-03-11T23:54:47Z |
| format | Article |
| id | doaj.art-142409104cd740089e2478e672cb3cab |
| institution | Directory Open Access Journal |
| issn | 0120-4157 |
| language | English |
| last_indexed | 2024-03-11T23:54:47Z |
| publishDate | 2023-08-01 |
| publisher | Instituto Nacional de Salud |
| record_format | Article |
| series | Biomédica: revista del Instituto Nacional de Salud |
| spelling | doaj.art-142409104cd740089e2478e672cb3cab2023-09-18T17:00:38ZengInstituto Nacional de SaludBiomédica: revista del Instituto Nacional de Salud0120-41572023-08-0143Sp. 121622810.7705/biomedica.68988353Phenotypic and genotypic characterization of Colombian clinical isolates of Sporothrix spp.Laura C. Álvarez-Acevedo0María C. Zuleta-González1Óscar M. Gómez-Guzmán2Álvaro L. Rúa-Giraldo3Orville Hernández-Ruiz4Juan G. McEwen-Ochoa5Martha E. Urán-Jiménez6Myrtha Arango-Arteaga7Rosely M. Zancopé-Oliveira8Manoel Marques Evangelista de Oliveira9María del P. Jiménez-Alzate10Grupo de Micología Médica, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Posgrado de Biología, Instituto de Biología, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, Medellín, ColombiaGrupo de Micología Médica, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, ColombiaPosgrado de Biología, Instituto de Biología, Facultad de Ciencias Exactas y Naturales, Universidad de Antioquia, Medellín, Colombia; Grupo de Biología Celular y Molecular, Corporación para Investigaciones Biológicas, Universidad de Antioquia, Medellín, ColombiaGrupo de Micología Médica, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Escuela de Microbiología, Universidad de Antioquia, Medellín, ColombiaGrupo de Biología Celular y Molecular, Corporación para Investigaciones Biológicas, Universidad de Antioquia, Medellín, Colombia; Escuela de Microbiología, Universidad de Antioquia, Medellín, ColombiaGrupo de Micología Médica, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia; Grupo de Biología Celular y Molecular, Corporación para Investigaciones Biológicas, Universidad de Antioquia, Medellín, ColombiaGrupo de Micología Médica, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, ColombiaGrupo de Micología Médica, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, ColombiaLaboratorio de Micología, Instituto Nacional de Infectología Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, BrazilLaboratorio de Micología, Instituto Nacional de Infectología Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, BrazilGrupo de Micología Médica, Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad de Antioquia, Medellín, ColombiaIntroduction. For over a century, Sporothrix schenckii was considered the sole species responsible for sporotrichosis. In 2007, scientific community confirmed the disease could be caused by various Sporothrix species. These species differed in their virulence factors and their antifungal sensitivity. Objective. This study aims to characterize 42 Colombian clinical isolates of Sporothrix spp. phenotypically and genotypically. Material and methods. Forty-two clinical isolates were characterized using phenotypic methods. It involved various culture media to determine their growth range at different temperatures and to assess the type and distribution of pigment and colony texture. Microscopic morphology was evaluated through microcultures, as well as the conidia diameter, type of sporulation, and morphology. Additionally, the assimilation of carbohydrates was selected as a physiological trait for species identification. Genotyping of 40 isolates was performed through partial amplification of the calmodulin gene, followed by sequence analysis. Results. Molecular studies enabled the identification of 32 isolates of S. schenckii and 8 isolates of S. globosa. The combination of phenotypic and genotypic methods eased these species characterizations and the recognition keys development based on parameters such as growth diameter at 25 and 30 ºC, colony texture (membranous or velvety) on potato dextrose agar, and microscopic morphology with predominance of pigmented triangular, elongated oval globose, or subglobose conidia. Conclusions. Confirmation of the phenotypic characteristics and molecular analysis is crucial for identifying Sporothrix species and determining adequate treatment. This study represents the first phenotypical and genotypical characterization of clinical isolates of Sporothrix spp. reported in Colombia.https://revistabiomedica.org/index.php/biomedica/article/view/6898sporothrixsporotrichosisphenotypegenotype |
| spellingShingle | Laura C. Álvarez-Acevedo María C. Zuleta-González Óscar M. Gómez-Guzmán Álvaro L. Rúa-Giraldo Orville Hernández-Ruiz Juan G. McEwen-Ochoa Martha E. Urán-Jiménez Myrtha Arango-Arteaga Rosely M. Zancopé-Oliveira Manoel Marques Evangelista de Oliveira María del P. Jiménez-Alzate Phenotypic and genotypic characterization of Colombian clinical isolates of Sporothrix spp. Biomédica: revista del Instituto Nacional de Salud sporothrix sporotrichosis phenotype genotype |
| title | Phenotypic and genotypic characterization of Colombian clinical isolates of Sporothrix spp. |
| title_full | Phenotypic and genotypic characterization of Colombian clinical isolates of Sporothrix spp. |
| title_fullStr | Phenotypic and genotypic characterization of Colombian clinical isolates of Sporothrix spp. |
| title_full_unstemmed | Phenotypic and genotypic characterization of Colombian clinical isolates of Sporothrix spp. |
| title_short | Phenotypic and genotypic characterization of Colombian clinical isolates of Sporothrix spp. |
| title_sort | phenotypic and genotypic characterization of colombian clinical isolates of sporothrix spp |
| topic | sporothrix sporotrichosis phenotype genotype |
| url | https://revistabiomedica.org/index.php/biomedica/article/view/6898 |
| work_keys_str_mv | AT lauracalvarezacevedo phenotypicandgenotypiccharacterizationofcolombianclinicalisolatesofsporothrixspp AT mariaczuletagonzalez phenotypicandgenotypiccharacterizationofcolombianclinicalisolatesofsporothrixspp AT oscarmgomezguzman phenotypicandgenotypiccharacterizationofcolombianclinicalisolatesofsporothrixspp AT alvarolruagiraldo phenotypicandgenotypiccharacterizationofcolombianclinicalisolatesofsporothrixspp AT orvillehernandezruiz phenotypicandgenotypiccharacterizationofcolombianclinicalisolatesofsporothrixspp AT juangmcewenochoa phenotypicandgenotypiccharacterizationofcolombianclinicalisolatesofsporothrixspp AT marthaeuranjimenez phenotypicandgenotypiccharacterizationofcolombianclinicalisolatesofsporothrixspp AT myrthaarangoarteaga phenotypicandgenotypiccharacterizationofcolombianclinicalisolatesofsporothrixspp AT roselymzancopeoliveira phenotypicandgenotypiccharacterizationofcolombianclinicalisolatesofsporothrixspp AT manoelmarquesevangelistadeoliveira phenotypicandgenotypiccharacterizationofcolombianclinicalisolatesofsporothrixspp AT mariadelpjimenezalzate phenotypicandgenotypiccharacterizationofcolombianclinicalisolatesofsporothrixspp |