Circulating miRNAs as Putative Biomarkers of Exercise Adaptation in Endurance Horses
Endurance exercise induces metabolic adaptations and has recently been reported associated with the modulation of a particular class of small noncoding RNAs, microRNAs, that act as post-transcriptional regulators of gene expression. Released into body fluids, they termed circulating miRNAs, and they...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Physiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2018.00429/full |
| _version_ | 1818322591946899456 |
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| author | Katia Cappelli Stefano Capomaccio Andrea Viglino Maurizio Silvestrelli Francesca Beccati Livia Moscati Elisabetta Chiaradia |
| author_facet | Katia Cappelli Stefano Capomaccio Andrea Viglino Maurizio Silvestrelli Francesca Beccati Livia Moscati Elisabetta Chiaradia |
| author_sort | Katia Cappelli |
| collection | DOAJ |
| description | Endurance exercise induces metabolic adaptations and has recently been reported associated with the modulation of a particular class of small noncoding RNAs, microRNAs, that act as post-transcriptional regulators of gene expression. Released into body fluids, they termed circulating miRNAs, and they have been recognized as more effective and accurate biomarkers than classical serum markers. This study examined serum profile of miRNAs through massive parallel sequencing in response to prolonged endurance exercise in samples obtained from four competitive Arabian horses before and 2 h after the end of competition. MicroRNA identification, differential gene expression (DGE) analysis and a protein-protein interaction (PPI) network showing significantly enriched pathways of target gene clusters, were assessed and explored. Our results show modulation of more than 100 miRNAs probably arising from tissues involved in exercise responses and indicating the modulation of correlated processes as muscle remodeling, immune and inflammatory responses. Circulating miRNA high-throughput sequencing is a promising approach for sports medicine for the discovery of putative biomarkers for predicting risks related to prolonged activity and monitoring metabolic adaptations. |
| first_indexed | 2024-12-13T10:59:14Z |
| format | Article |
| id | doaj.art-14251e424c6843ddb0e990bfb58e00c5 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-13T10:59:14Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-14251e424c6843ddb0e990bfb58e00c52022-12-21T23:49:18ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-04-01910.3389/fphys.2018.00429314690Circulating miRNAs as Putative Biomarkers of Exercise Adaptation in Endurance HorsesKatia Cappelli0Stefano Capomaccio1Andrea Viglino2Maurizio Silvestrelli3Francesca Beccati4Livia Moscati5Elisabetta Chiaradia6Dipartimento di Medicina Veterinaria, Centro di Studio del Cavallo Sportivo, University of Perugia, Perugia, ItalyDipartimento di Medicina Veterinaria, Centro di Studio del Cavallo Sportivo, University of Perugia, Perugia, ItalyFacoltà di Scienze Agrarie, Alimentari e Ambientali, Istituto di Zootecnica, Università Cattolica del Sacro Cuore, Piacenza, ItalyDipartimento di Medicina Veterinaria, Centro di Studio del Cavallo Sportivo, University of Perugia, Perugia, ItalyDipartimento di Medicina Veterinaria, Centro di Studio del Cavallo Sportivo, University of Perugia, Perugia, ItalyIstituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Perugia, ItalyDipartimento di Medicina Veterinaria, Centro di Studio del Cavallo Sportivo, University of Perugia, Perugia, ItalyEndurance exercise induces metabolic adaptations and has recently been reported associated with the modulation of a particular class of small noncoding RNAs, microRNAs, that act as post-transcriptional regulators of gene expression. Released into body fluids, they termed circulating miRNAs, and they have been recognized as more effective and accurate biomarkers than classical serum markers. This study examined serum profile of miRNAs through massive parallel sequencing in response to prolonged endurance exercise in samples obtained from four competitive Arabian horses before and 2 h after the end of competition. MicroRNA identification, differential gene expression (DGE) analysis and a protein-protein interaction (PPI) network showing significantly enriched pathways of target gene clusters, were assessed and explored. Our results show modulation of more than 100 miRNAs probably arising from tissues involved in exercise responses and indicating the modulation of correlated processes as muscle remodeling, immune and inflammatory responses. Circulating miRNA high-throughput sequencing is a promising approach for sports medicine for the discovery of putative biomarkers for predicting risks related to prolonged activity and monitoring metabolic adaptations.http://journal.frontiersin.org/article/10.3389/fphys.2018.00429/fullmiRNAenduranceNGSbiomarkershorses |
| spellingShingle | Katia Cappelli Stefano Capomaccio Andrea Viglino Maurizio Silvestrelli Francesca Beccati Livia Moscati Elisabetta Chiaradia Circulating miRNAs as Putative Biomarkers of Exercise Adaptation in Endurance Horses Frontiers in Physiology miRNA endurance NGS biomarkers horses |
| title | Circulating miRNAs as Putative Biomarkers of Exercise Adaptation in Endurance Horses |
| title_full | Circulating miRNAs as Putative Biomarkers of Exercise Adaptation in Endurance Horses |
| title_fullStr | Circulating miRNAs as Putative Biomarkers of Exercise Adaptation in Endurance Horses |
| title_full_unstemmed | Circulating miRNAs as Putative Biomarkers of Exercise Adaptation in Endurance Horses |
| title_short | Circulating miRNAs as Putative Biomarkers of Exercise Adaptation in Endurance Horses |
| title_sort | circulating mirnas as putative biomarkers of exercise adaptation in endurance horses |
| topic | miRNA endurance NGS biomarkers horses |
| url | http://journal.frontiersin.org/article/10.3389/fphys.2018.00429/full |
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