Microphotokinesigraphic analysis of buffalo spermatozoa I. Characteristic features and significance of the immobile buffalo spermatozoa
The article discusses the results of a study of immobile spermatozoa of buffalo bulls from Bulgarian murrah breed, based on photo images registered in various displays in conditions of dark background photokinesis. The analysis showed the way developer affects the brightness of kinesigrams. The stud...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2010-02-01
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| Series: | Italian Journal of Animal Science |
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| Online Access: | http://www.aspajournal.it/index.php/ijas/article/view/1106 |
| Summary: | The article discusses the results of a study of immobile spermatozoa of buffalo bulls from Bulgarian murrah breed, based on photo images registered in various displays in conditions of dark background photokinesis. The analysis showed the way developer affects the brightness of kinesigrams. The study is based on samples of semen of six buffalos analysed by means of the microphotokinetic method developed by Tchakarov and Natchev. Brightness parameters of the cells and their photokinetic images have been established photometrically; their metric parameters have been defined by means of an eyepiece and object micrometer. It has been found out that the optimal exposure time for a photokinetic analysis of buffalo spermatozoa was(is) 5 sec, and the recommended developer for treating of negative registration material is the pyrocatechin developer by Windisch. |
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| ISSN: | 1594-4077 1828-051X |