<i>Pulicaria incisa</i> (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis
Plants with medicinal benefits are a crucial source of compounds for developing drugs. This study was designed to determine the chemical composition, antibacterial, antibiofilm, antioxidant, and anti-enzymatic activities of <i>Pulicaria incisa</i> (Lam.) DC. We also reported the molecula...
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MDPI AG
2023-11-01
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| author | Mousa Alreshidi Mohammad A. Abdulhakeem Riadh Badraoui Giuseppe Amato Lucia Caputo Laura De Martino Filomena Nazzaro Florinda Fratianni Carmen Formisano Vincenzo De Feo Mejdi Snoussi |
| author_facet | Mousa Alreshidi Mohammad A. Abdulhakeem Riadh Badraoui Giuseppe Amato Lucia Caputo Laura De Martino Filomena Nazzaro Florinda Fratianni Carmen Formisano Vincenzo De Feo Mejdi Snoussi |
| author_sort | Mousa Alreshidi |
| collection | DOAJ |
| description | Plants with medicinal benefits are a crucial source of compounds for developing drugs. This study was designed to determine the chemical composition, antibacterial, antibiofilm, antioxidant, and anti-enzymatic activities of <i>Pulicaria incisa</i> (Lam.) DC. We also reported the molecular interaction between identified molecules and several receptors associated with antimicrobial and antibiofilm activities. A total of seventeen and thirteen compounds were identified in aqueous and methanolic extracts of <i>P. incisa</i>, respectively. The methanolic extract yielded a higher total content of polyphenols and flavonoids of about 84.80 ± 2.8 mg GAE/g and 28.30 ± 1.2 mg QE/g, respectively. Significant antibacterial activity was recorded for both extracts, with minimum inhibitory concentration (MIC) values ranging from 30 to 36 µg/mL, and the result was comparable to the reference antibiotic control. Antibiofilm assays revealed that both extracts were able to reduce the attachment of bacterial cells to 96-well plates, but the highest antibiofilm activity was recorded against <i>Staphylococcus aureus</i>. The methanolic extract also showed anti-enzymatic potency and high antioxidant activity, as demonstrated by all assays used, including DPPH, FRAP, and ABTS. These results were further validated by in silico approaches, particularly the molecular interaction of the identified compounds with the targeted receptors. These findings present <i>P. incisa</i> as a significant source of antibacterial, antibiofilm, antioxidant, and anti-enzymatic molecules. |
| first_indexed | 2024-03-11T11:24:01Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T11:24:01Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-1429f9786ce64583ba1df0ab77a0c8532023-11-10T15:09:00ZengMDPI AGMolecules1420-30492023-11-012821743910.3390/molecules28217439<i>Pulicaria incisa</i> (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological AnalysisMousa Alreshidi0Mohammad A. Abdulhakeem1Riadh Badraoui2Giuseppe Amato3Lucia Caputo4Laura De Martino5Filomena Nazzaro6Florinda Fratianni7Carmen Formisano8Vincenzo De Feo9Mejdi Snoussi10Department of Biology, College of Science, University of Ha’il, Hail 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Hail 2440, Saudi ArabiaDepartment of Biology, College of Science, University of Ha’il, Hail 2440, Saudi ArabiaDepartment of Pharmacy, University of Salerno, 84084 Fisciano, ItalyDepartment of Pharmacy, University of Salerno, 84084 Fisciano, ItalyDepartment of Pharmacy, University of Salerno, 84084 Fisciano, ItalyInstitute of Food Sciences, CNR, 83100 Avellino, ItalyInstitute of Food Sciences, CNR, 83100 Avellino, ItalyDepartment of Pharmacy, School of Medicine and Surgery, University of Napoli Federico II, 80131 Napoli, ItalyDepartment of Pharmacy, University of Salerno, 84084 Fisciano, ItalyDepartment of Biology, College of Science, University of Ha’il, Hail 2440, Saudi ArabiaPlants with medicinal benefits are a crucial source of compounds for developing drugs. This study was designed to determine the chemical composition, antibacterial, antibiofilm, antioxidant, and anti-enzymatic activities of <i>Pulicaria incisa</i> (Lam.) DC. We also reported the molecular interaction between identified molecules and several receptors associated with antimicrobial and antibiofilm activities. A total of seventeen and thirteen compounds were identified in aqueous and methanolic extracts of <i>P. incisa</i>, respectively. The methanolic extract yielded a higher total content of polyphenols and flavonoids of about 84.80 ± 2.8 mg GAE/g and 28.30 ± 1.2 mg QE/g, respectively. Significant antibacterial activity was recorded for both extracts, with minimum inhibitory concentration (MIC) values ranging from 30 to 36 µg/mL, and the result was comparable to the reference antibiotic control. Antibiofilm assays revealed that both extracts were able to reduce the attachment of bacterial cells to 96-well plates, but the highest antibiofilm activity was recorded against <i>Staphylococcus aureus</i>. The methanolic extract also showed anti-enzymatic potency and high antioxidant activity, as demonstrated by all assays used, including DPPH, FRAP, and ABTS. These results were further validated by in silico approaches, particularly the molecular interaction of the identified compounds with the targeted receptors. These findings present <i>P. incisa</i> as a significant source of antibacterial, antibiofilm, antioxidant, and anti-enzymatic molecules.https://www.mdpi.com/1420-3049/28/21/7439<i>P. incisa</i> extractphytochemical compositionantibacterialantioxidant activitiesanti-enzymatic activitiesADME |
| spellingShingle | Mousa Alreshidi Mohammad A. Abdulhakeem Riadh Badraoui Giuseppe Amato Lucia Caputo Laura De Martino Filomena Nazzaro Florinda Fratianni Carmen Formisano Vincenzo De Feo Mejdi Snoussi <i>Pulicaria incisa</i> (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis Molecules <i>P. incisa</i> extract phytochemical composition antibacterial antioxidant activities anti-enzymatic activities ADME |
| title | <i>Pulicaria incisa</i> (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis |
| title_full | <i>Pulicaria incisa</i> (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis |
| title_fullStr | <i>Pulicaria incisa</i> (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis |
| title_full_unstemmed | <i>Pulicaria incisa</i> (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis |
| title_short | <i>Pulicaria incisa</i> (Lam.) DC. as a Potential Source of Antioxidant, Antibacterial, and Anti-Enzymatic Bioactive Molecules: Phytochemical Constituents, In Vitro and In Silico Pharmacological Analysis |
| title_sort | i pulicaria incisa i lam dc as a potential source of antioxidant antibacterial and anti enzymatic bioactive molecules phytochemical constituents in vitro and in silico pharmacological analysis |
| topic | <i>P. incisa</i> extract phytochemical composition antibacterial antioxidant activities anti-enzymatic activities ADME |
| url | https://www.mdpi.com/1420-3049/28/21/7439 |
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