Dosage compensation of Z sex chromosome genes in avian fibroblast cells
Abstract In birds, sex is genetically determined; however, the molecular mechanism is not well-understood. The avian Z sex chromosome (chrZ) lacks whole chromosome inactivation, in contrast to the mammalian chrX. To investigate chrZ dosage compensation and its role in sex specification, we use a hig...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-09-01
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| Series: | Genome Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13059-023-03055-z |
| _version_ | 1797452420418109440 |
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| author | Ruslan Deviatiiarov Hiroki Nagai Galym Ismagulov Anastasia Stupina Kazuhiro Wada Shinji Ide Noriyuki Toji Heng Zhang Woranop Sukparangsi Sittipon Intarapat Oleg Gusev Guojun Sheng |
| author_facet | Ruslan Deviatiiarov Hiroki Nagai Galym Ismagulov Anastasia Stupina Kazuhiro Wada Shinji Ide Noriyuki Toji Heng Zhang Woranop Sukparangsi Sittipon Intarapat Oleg Gusev Guojun Sheng |
| author_sort | Ruslan Deviatiiarov |
| collection | DOAJ |
| description | Abstract In birds, sex is genetically determined; however, the molecular mechanism is not well-understood. The avian Z sex chromosome (chrZ) lacks whole chromosome inactivation, in contrast to the mammalian chrX. To investigate chrZ dosage compensation and its role in sex specification, we use a highly quantitative method and analyze transcriptional activities of male and female fibroblast cells from seven bird species. Our data indicate that three fourths of chrZ genes are strictly compensated across Aves, similar to mammalian chrX. We also present a complete list of non-compensated chrZ genes and identify Ribosomal Protein S6 (RPS6) as a conserved sex-dimorphic gene in birds. |
| first_indexed | 2024-03-09T15:08:30Z |
| format | Article |
| id | doaj.art-142a8b265a0a4cc482b4a5c1deafe764 |
| institution | Directory Open Access Journal |
| issn | 1474-760X |
| language | English |
| last_indexed | 2024-03-09T15:08:30Z |
| publishDate | 2023-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Genome Biology |
| spelling | doaj.art-142a8b265a0a4cc482b4a5c1deafe7642023-11-26T13:29:32ZengBMCGenome Biology1474-760X2023-09-0124111110.1186/s13059-023-03055-zDosage compensation of Z sex chromosome genes in avian fibroblast cellsRuslan Deviatiiarov0Hiroki Nagai1Galym Ismagulov2Anastasia Stupina3Kazuhiro Wada4Shinji Ide5Noriyuki Toji6Heng Zhang7Woranop Sukparangsi8Sittipon Intarapat9Oleg Gusev10Guojun Sheng11International Research Center for Medical Sciences, Kumamoto UniversityInternational Research Center for Medical Sciences, Kumamoto UniversityInternational Research Center for Medical Sciences, Kumamoto UniversityRegulatory Genomics Research Center, Institute of Fundamental Medicine and Biology, Kazan Federal UniversityDepartment of Biological Sciences, Faculty of Science, Hokkaido UniversityKumamoto City Zoo and Botanical GardenDepartment of Biological Sciences, Faculty of Science, Hokkaido UniversityGraduate School of Life Science, Hokkaido UniversityDepartment of Biology, Faculty of Science, Burapha UniversityFaculty of Science, Mahidol UniversityRegulatory Genomics Research Center, Institute of Fundamental Medicine and Biology, Kazan Federal UniversityInternational Research Center for Medical Sciences, Kumamoto UniversityAbstract In birds, sex is genetically determined; however, the molecular mechanism is not well-understood. The avian Z sex chromosome (chrZ) lacks whole chromosome inactivation, in contrast to the mammalian chrX. To investigate chrZ dosage compensation and its role in sex specification, we use a highly quantitative method and analyze transcriptional activities of male and female fibroblast cells from seven bird species. Our data indicate that three fourths of chrZ genes are strictly compensated across Aves, similar to mammalian chrX. We also present a complete list of non-compensated chrZ genes and identify Ribosomal Protein S6 (RPS6) as a conserved sex-dimorphic gene in birds.https://doi.org/10.1186/s13059-023-03055-zBirdsSex chromosomeDosage compensationSex determinationFibroblastCAGE (cap analysis of gene expression) |
| spellingShingle | Ruslan Deviatiiarov Hiroki Nagai Galym Ismagulov Anastasia Stupina Kazuhiro Wada Shinji Ide Noriyuki Toji Heng Zhang Woranop Sukparangsi Sittipon Intarapat Oleg Gusev Guojun Sheng Dosage compensation of Z sex chromosome genes in avian fibroblast cells Genome Biology Birds Sex chromosome Dosage compensation Sex determination Fibroblast CAGE (cap analysis of gene expression) |
| title | Dosage compensation of Z sex chromosome genes in avian fibroblast cells |
| title_full | Dosage compensation of Z sex chromosome genes in avian fibroblast cells |
| title_fullStr | Dosage compensation of Z sex chromosome genes in avian fibroblast cells |
| title_full_unstemmed | Dosage compensation of Z sex chromosome genes in avian fibroblast cells |
| title_short | Dosage compensation of Z sex chromosome genes in avian fibroblast cells |
| title_sort | dosage compensation of z sex chromosome genes in avian fibroblast cells |
| topic | Birds Sex chromosome Dosage compensation Sex determination Fibroblast CAGE (cap analysis of gene expression) |
| url | https://doi.org/10.1186/s13059-023-03055-z |
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