Bioactive Chemical Constituents of <i>Matthiola longipetala</i> Extract Showed Antioxidant, Antibacterial, and Cytotoxic Potency
The exploration of bioactive compounds from natural resources attracts the attention of researchers and scientists worldwide. <i>M. longipetala</i> is an annual aromatic herb that emits a pleasant odor during the night. Regarding the chemical composition and biological characteristics, &...
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MDPI AG
2023-01-01
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| Online Access: | https://www.mdpi.com/2297-8739/10/1/53 |
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| author | Yasser A. El-Amier Nouf S. Zaghloul Ahmed M. Abd-ElGawad |
| author_facet | Yasser A. El-Amier Nouf S. Zaghloul Ahmed M. Abd-ElGawad |
| author_sort | Yasser A. El-Amier |
| collection | DOAJ |
| description | The exploration of bioactive compounds from natural resources attracts the attention of researchers and scientists worldwide. <i>M. longipetala</i> is an annual aromatic herb that emits a pleasant odor during the night. Regarding the chemical composition and biological characteristics, <i>M. longipetala</i> extracts are poorly studied. The current study aimed to characterize the chemical composition of <i>M. longipetala</i> methanol extract using GC-MS and determine its biological potencies, including its capacity for cytotoxicity and antioxidant and antibacterial activities. In this approach, 37 components were identified, representing 99.98% of the total mass. The major chemical components can be classified as oxygenated hydrocarbons (19.15%), carbohydrates (10.21%), amines (4.85%), terpenoids (12.71%), fatty acids and lipids (50.8%), and steroids (2.26%). The major identified compounds were ascaridole epoxide (monoterpene, 12.71%) and methyl (<i>E</i>)-octadec-11-enoate (ester of fatty acid, 12.21%). The extract of <i>M. longipetala</i> showed substantial antioxidant activity. Based on the DPPH and ABTS scavenging, the antioxidant activity of the extracted components of <i>M. longipetala</i> revealed that leaf extract is the most effective with IC<sub>50</sub> values of 31.47 and 28.94 mg/L, respectively. On the other hand, the extracted plant showed low antibacterial activities against diverse bacterial species, viz., <i>Escherichia coli</i>, <i>Klebsiella pneumonia</i>, <i>Staphylococcus epidermidis</i>, <i>S. haemolyticus</i>, and <i>S. aureus</i>. The most potent antibacterial results were documented for leaf and flower extracts against <i>E. coli</i> and <i>S. aureus</i>. Additionally, the extract’s effectiveness against HepG2 cells was evaluated in vitro using the measures of MTT, DNA fragmentation, and cell proliferation cycle, where it showed considerable activity. Therefore, we can conclude that <i>M. longipetala</i> extract displayed improvement in cytocompatibility and cell migration properties. In conclusion, <i>M. longipetala</i> could be considered a potential candidate for various bioactive compounds with promising biological activities. However, further characterization of the identified compounds, particularly the major compounds, is recommended to evaluate their efficacy, modes of action, and safety. |
| first_indexed | 2024-03-09T11:14:36Z |
| format | Article |
| id | doaj.art-142b46ef9b3348aca116e99a4cb45141 |
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| language | English |
| last_indexed | 2024-03-09T11:14:36Z |
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| spelling | doaj.art-142b46ef9b3348aca116e99a4cb451412023-12-01T00:32:52ZengMDPI AGSeparations2297-87392023-01-011015310.3390/separations10010053Bioactive Chemical Constituents of <i>Matthiola longipetala</i> Extract Showed Antioxidant, Antibacterial, and Cytotoxic PotencyYasser A. El-Amier0Nouf S. Zaghloul1Ahmed M. Abd-ElGawad2Department of Botany, Faculty of Science, Mansoura University, Mansoura 35516, EgyptBristol Centre for Functional Nanomaterials, HH Wills Physics Laboratory, Tyndall Avenue, Bristol BS8 1FD, UKDepartment of Plant Production, College of Food & Agriculture Sciences, King Saud University, P.O. Box 2460, Riyadh 11451, Saudi ArabiaThe exploration of bioactive compounds from natural resources attracts the attention of researchers and scientists worldwide. <i>M. longipetala</i> is an annual aromatic herb that emits a pleasant odor during the night. Regarding the chemical composition and biological characteristics, <i>M. longipetala</i> extracts are poorly studied. The current study aimed to characterize the chemical composition of <i>M. longipetala</i> methanol extract using GC-MS and determine its biological potencies, including its capacity for cytotoxicity and antioxidant and antibacterial activities. In this approach, 37 components were identified, representing 99.98% of the total mass. The major chemical components can be classified as oxygenated hydrocarbons (19.15%), carbohydrates (10.21%), amines (4.85%), terpenoids (12.71%), fatty acids and lipids (50.8%), and steroids (2.26%). The major identified compounds were ascaridole epoxide (monoterpene, 12.71%) and methyl (<i>E</i>)-octadec-11-enoate (ester of fatty acid, 12.21%). The extract of <i>M. longipetala</i> showed substantial antioxidant activity. Based on the DPPH and ABTS scavenging, the antioxidant activity of the extracted components of <i>M. longipetala</i> revealed that leaf extract is the most effective with IC<sub>50</sub> values of 31.47 and 28.94 mg/L, respectively. On the other hand, the extracted plant showed low antibacterial activities against diverse bacterial species, viz., <i>Escherichia coli</i>, <i>Klebsiella pneumonia</i>, <i>Staphylococcus epidermidis</i>, <i>S. haemolyticus</i>, and <i>S. aureus</i>. The most potent antibacterial results were documented for leaf and flower extracts against <i>E. coli</i> and <i>S. aureus</i>. Additionally, the extract’s effectiveness against HepG2 cells was evaluated in vitro using the measures of MTT, DNA fragmentation, and cell proliferation cycle, where it showed considerable activity. Therefore, we can conclude that <i>M. longipetala</i> extract displayed improvement in cytocompatibility and cell migration properties. In conclusion, <i>M. longipetala</i> could be considered a potential candidate for various bioactive compounds with promising biological activities. However, further characterization of the identified compounds, particularly the major compounds, is recommended to evaluate their efficacy, modes of action, and safety.https://www.mdpi.com/2297-8739/10/1/53BrassicaceaeGC-MSbioactive compoundsantioxidant activityascaridole epoxide |
| spellingShingle | Yasser A. El-Amier Nouf S. Zaghloul Ahmed M. Abd-ElGawad Bioactive Chemical Constituents of <i>Matthiola longipetala</i> Extract Showed Antioxidant, Antibacterial, and Cytotoxic Potency Separations Brassicaceae GC-MS bioactive compounds antioxidant activity ascaridole epoxide |
| title | Bioactive Chemical Constituents of <i>Matthiola longipetala</i> Extract Showed Antioxidant, Antibacterial, and Cytotoxic Potency |
| title_full | Bioactive Chemical Constituents of <i>Matthiola longipetala</i> Extract Showed Antioxidant, Antibacterial, and Cytotoxic Potency |
| title_fullStr | Bioactive Chemical Constituents of <i>Matthiola longipetala</i> Extract Showed Antioxidant, Antibacterial, and Cytotoxic Potency |
| title_full_unstemmed | Bioactive Chemical Constituents of <i>Matthiola longipetala</i> Extract Showed Antioxidant, Antibacterial, and Cytotoxic Potency |
| title_short | Bioactive Chemical Constituents of <i>Matthiola longipetala</i> Extract Showed Antioxidant, Antibacterial, and Cytotoxic Potency |
| title_sort | bioactive chemical constituents of i matthiola longipetala i extract showed antioxidant antibacterial and cytotoxic potency |
| topic | Brassicaceae GC-MS bioactive compounds antioxidant activity ascaridole epoxide |
| url | https://www.mdpi.com/2297-8739/10/1/53 |
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