Diet Quality and Liver Health in People Living with HIV in the MASH Cohort: A Multi-Omic Analysis of the Fecal Microbiome and Metabolome
The gut–liver axis has been recognized as a potential pathway in which dietary factors may contribute to liver disease in people living with HIV (PLWH). The objective of this study was to explore associations between dietary quality, the fecal microbiome, the metabolome, and liver health in PLWH fro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/2/271 |
| _version_ | 1797619280456450048 |
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| author | Haley R. Martin Sabrina Sales Martinez Vitalii Stebliankin Javier A. Tamargo Adriana Campa Giri Narasimhan Jacqueline Hernandez Jose A. Bastida Rodriguez Colby Teeman Angelique Johnson Kenneth E. Sherman Marianna K. Baum |
| author_facet | Haley R. Martin Sabrina Sales Martinez Vitalii Stebliankin Javier A. Tamargo Adriana Campa Giri Narasimhan Jacqueline Hernandez Jose A. Bastida Rodriguez Colby Teeman Angelique Johnson Kenneth E. Sherman Marianna K. Baum |
| author_sort | Haley R. Martin |
| collection | DOAJ |
| description | The gut–liver axis has been recognized as a potential pathway in which dietary factors may contribute to liver disease in people living with HIV (PLWH). The objective of this study was to explore associations between dietary quality, the fecal microbiome, the metabolome, and liver health in PLWH from the Miami Adult Studies on HIV (MASH) cohort. We performed a cross-sectional analysis of 50 PLWH from the MASH cohort and utilized the USDA Healthy Eating Index (HEI)–2015 to measure diet quality. A Fibrosis-4 Index (FIB-4) score < 1.45 was used as a strong indication that advanced liver fibrosis was not present. Stool samples and fasting blood plasma samples were collected. Bacterial composition was characterized using 16S rRNA sequencing. Metabolomics in plasma were determined using gas and liquid chromatography/mass spectrometry. Statistical analyses included biomarker identification using linear discriminant analysis effect size. Compared to participants with FIB-4 ≥ 1.45, participants with FIB-4 < 1.45 had higher intake of dairy (<i>p</i> = 0.006). Fibrosis-4 Index score was inversely correlated with seafood and plant protein HEI component score (<i>r</i> = −0.320, <i>p</i> = 0.022). The relative abundances of butyrate-producing taxa <i>Ruminococcaceae, Roseburia</i>, and <i>Lachnospiraceae</i> were higher in participants with FIB-4 < 1.45. Participants with FIB-4 < 1.45 also had higher levels of caffeine (<i>p</i> = 0.045) and related metabolites such as trigonelline (<i>p</i> = 0.008) and 1-methylurate (<i>p</i> = 0.023). Dietary components appear to be associated with the fecal microbiome and metabolome, and liver health in PLWH. Future studies should investigate whether targeting specific dietary components may reduce liver-related morbidity and mortality in PLWH. |
| first_indexed | 2024-03-11T08:25:54Z |
| format | Article |
| id | doaj.art-142cdbdc9d974abe95159fc13ee71d91 |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T08:25:54Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-142cdbdc9d974abe95159fc13ee71d912023-11-16T22:05:16ZengMDPI AGMetabolites2218-19892023-02-0113227110.3390/metabo13020271Diet Quality and Liver Health in People Living with HIV in the MASH Cohort: A Multi-Omic Analysis of the Fecal Microbiome and MetabolomeHaley R. Martin0Sabrina Sales Martinez1Vitalii Stebliankin2Javier A. Tamargo3Adriana Campa4Giri Narasimhan5Jacqueline Hernandez6Jose A. Bastida Rodriguez7Colby Teeman8Angelique Johnson9Kenneth E. Sherman10Marianna K. Baum11Robert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, AHC-5 500, Miami, FL 33199, USARobert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, AHC-5 500, Miami, FL 33199, USABioinformatics Research Group (BioRG), School of Computing and Information Sciences, University Park Campus, Florida International University, ECS-254, Miami, FL 33199, USARobert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, AHC-5 500, Miami, FL 33199, USARobert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, AHC-5 500, Miami, FL 33199, USABioinformatics Research Group (BioRG), School of Computing and Information Sciences, University Park Campus, Florida International University, ECS-254, Miami, FL 33199, USARobert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, AHC-5 500, Miami, FL 33199, USARobert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, AHC-5 500, Miami, FL 33199, USARobert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, AHC-5 500, Miami, FL 33199, USARobert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, AHC-5 500, Miami, FL 33199, USADepartment of Internal Medicine, Division of Digestive Diseases, College of Medicine, University of Cincinnati, 3230 Eden Ave, Cincinnati, OH 45267, USARobert Stempel College of Public Health and Social Work, Florida International University, 11200 SW 8th Street, AHC-5 500, Miami, FL 33199, USAThe gut–liver axis has been recognized as a potential pathway in which dietary factors may contribute to liver disease in people living with HIV (PLWH). The objective of this study was to explore associations between dietary quality, the fecal microbiome, the metabolome, and liver health in PLWH from the Miami Adult Studies on HIV (MASH) cohort. We performed a cross-sectional analysis of 50 PLWH from the MASH cohort and utilized the USDA Healthy Eating Index (HEI)–2015 to measure diet quality. A Fibrosis-4 Index (FIB-4) score < 1.45 was used as a strong indication that advanced liver fibrosis was not present. Stool samples and fasting blood plasma samples were collected. Bacterial composition was characterized using 16S rRNA sequencing. Metabolomics in plasma were determined using gas and liquid chromatography/mass spectrometry. Statistical analyses included biomarker identification using linear discriminant analysis effect size. Compared to participants with FIB-4 ≥ 1.45, participants with FIB-4 < 1.45 had higher intake of dairy (<i>p</i> = 0.006). Fibrosis-4 Index score was inversely correlated with seafood and plant protein HEI component score (<i>r</i> = −0.320, <i>p</i> = 0.022). The relative abundances of butyrate-producing taxa <i>Ruminococcaceae, Roseburia</i>, and <i>Lachnospiraceae</i> were higher in participants with FIB-4 < 1.45. Participants with FIB-4 < 1.45 also had higher levels of caffeine (<i>p</i> = 0.045) and related metabolites such as trigonelline (<i>p</i> = 0.008) and 1-methylurate (<i>p</i> = 0.023). Dietary components appear to be associated with the fecal microbiome and metabolome, and liver health in PLWH. Future studies should investigate whether targeting specific dietary components may reduce liver-related morbidity and mortality in PLWH.https://www.mdpi.com/2218-1989/13/2/271HIVmetabolomemetabolomicsmicrobiomeliver diseasediet |
| spellingShingle | Haley R. Martin Sabrina Sales Martinez Vitalii Stebliankin Javier A. Tamargo Adriana Campa Giri Narasimhan Jacqueline Hernandez Jose A. Bastida Rodriguez Colby Teeman Angelique Johnson Kenneth E. Sherman Marianna K. Baum Diet Quality and Liver Health in People Living with HIV in the MASH Cohort: A Multi-Omic Analysis of the Fecal Microbiome and Metabolome Metabolites HIV metabolome metabolomics microbiome liver disease diet |
| title | Diet Quality and Liver Health in People Living with HIV in the MASH Cohort: A Multi-Omic Analysis of the Fecal Microbiome and Metabolome |
| title_full | Diet Quality and Liver Health in People Living with HIV in the MASH Cohort: A Multi-Omic Analysis of the Fecal Microbiome and Metabolome |
| title_fullStr | Diet Quality and Liver Health in People Living with HIV in the MASH Cohort: A Multi-Omic Analysis of the Fecal Microbiome and Metabolome |
| title_full_unstemmed | Diet Quality and Liver Health in People Living with HIV in the MASH Cohort: A Multi-Omic Analysis of the Fecal Microbiome and Metabolome |
| title_short | Diet Quality and Liver Health in People Living with HIV in the MASH Cohort: A Multi-Omic Analysis of the Fecal Microbiome and Metabolome |
| title_sort | diet quality and liver health in people living with hiv in the mash cohort a multi omic analysis of the fecal microbiome and metabolome |
| topic | HIV metabolome metabolomics microbiome liver disease diet |
| url | https://www.mdpi.com/2218-1989/13/2/271 |
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