Qingre Jiangni formula improves esophageal epithelial barrier function in gastroesophageal reflux rats through male hormones and the p38/MAPK pathway
The incidence rate of gastroesophageal reflux is high. Qingre Jiangni Formula has shown good clinical efficacy. In this study, the therapeutic effect of this formula on inflammation was analyzed in the gastroesophageal reflux model. A rat model of gastroesophageal reflux was constructed using sur...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
MRE Press
2024-01-01
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Series: | Journal of Men's Health |
Subjects: | |
Online Access: | https://oss.jomh.org/files/article/20230928-100/pdf/JOMH2023091202.pdf |
Summary: | The incidence rate of gastroesophageal reflux is high. Qingre Jiangni Formula
has shown good clinical efficacy. In this study, the therapeutic effect of this
formula on inflammation was analyzed in the gastroesophageal reflux model. A rat
model of gastroesophageal reflux was constructed using surgical methods. The
successful construction of the rat model was confirmed through case testing. The
model rats were treated with Qingre herbs, Jiangni herbs, and Qingre + Jiangni
herbs for 2 weeks, followed by hematoxylin-eosin staining (HE), Western
Blot (WB), enzyme linked immunosorbent assay (Elisa) and polymerase chain
reaction (PCR) assays. SPSS 22.0 statistical software was used for analysis. The
modeling of gastroesophageal reflux reduced the levels of testosterone and
androsterol. The Qingre Jiangni formula helped to improve testosterone and
androsterol to normal levels. Treatments in the Qingre group, Jiangni group, and
Qingre + Jiangni group can all decrease inflammatory factor levels (interleukin
IL-1B, IL-6, IL-8), and lead to a decreasing trend in p38, Claudin-4, Occludin
and ZO_S. In addition, the three groups also showed decreased inflammation
pathological levels compared with those in the model group (p <
0.01). Qingre Jiangni Formula is helpful for male hormones (testosterone
and androsterol) and can decrease gastroesophageal reflux disease inflammation,
thereby treating gastroesophageal reflux disease. |
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ISSN: | 1875-6867 1875-6859 |