Cork Oak Forests Soil Bacteria: Potential for Sustainable Agroforest Production
Plant growth promoting rhizobacteria (PGPR) are in increasing demand due to their role in promoting sustainable practices, not only in agriculture but also in forestry. Keeping in mind the future application of PGPR for increasing cork oak sustainability, the aim of this study was to find cork oak P...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/9/9/1973 |
| _version_ | 1797518054782926848 |
|---|---|
| author | Francisca Reis Ana João Pereira Rui M. Tavares Paula Baptista Teresa Lino-Neto |
| author_facet | Francisca Reis Ana João Pereira Rui M. Tavares Paula Baptista Teresa Lino-Neto |
| author_sort | Francisca Reis |
| collection | DOAJ |
| description | Plant growth promoting rhizobacteria (PGPR) are in increasing demand due to their role in promoting sustainable practices, not only in agriculture but also in forestry. Keeping in mind the future application of PGPR for increasing cork oak sustainability, the aim of this study was to find cork oak PGPR isolates with increased nutrient solubilisation traits, able to promote root morphological changes and/or antagonize cork oak bark phytopathogens. Soils from three cork oak forests with distinct bioclimates (humid, semi-humid and semi-arid) were used for isolating bacteria. From the 7634 colony-forming units, 323 bacterial isolates were biochemically assayed for PGPR traits (siderophores production, phosphate solubilizing and organic acids production), and 51 were found to display all these traits. These PGPR were able to induce root morphological changes on <i>Arabidopsis thaliana</i>, like suppression of primary root growth, increase of lateral roots or root hairs formation. However, the most proficient PGPR displayed specific ability in changing a single root morphological trait. This ability was related not only to bacterial genotype, but also with the environment where bacteria thrived and isolation temperature. Bacteria from semi-arid environments (mainly <i>Bacillus megaterium</i> isolates) could hold a promising tool to enhance plant development. Other isolates (<i>Serratia quinivorens</i> or <i>B. cereus</i>) could be further explored for biocontrol purposes. |
| first_indexed | 2024-03-10T07:24:47Z |
| format | Article |
| id | doaj.art-1432098dda804682b13cc8912326b84a |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T07:24:47Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-1432098dda804682b13cc8912326b84a2023-11-22T14:19:57ZengMDPI AGMicroorganisms2076-26072021-09-0199197310.3390/microorganisms9091973Cork Oak Forests Soil Bacteria: Potential for Sustainable Agroforest ProductionFrancisca Reis0Ana João Pereira1Rui M. Tavares2Paula Baptista3Teresa Lino-Neto4Plant Functional Biology Centre, BioSystems and Integrative Sciences Institute (BioISI), Campus de Gualtar, University of Minho, 4710-057 Braga, PortugalPlant Functional Biology Centre, BioSystems and Integrative Sciences Institute (BioISI), Campus de Gualtar, University of Minho, 4710-057 Braga, PortugalPlant Functional Biology Centre, BioSystems and Integrative Sciences Institute (BioISI), Campus de Gualtar, University of Minho, 4710-057 Braga, PortugalCentro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253 Bragança, PortugalPlant Functional Biology Centre, BioSystems and Integrative Sciences Institute (BioISI), Campus de Gualtar, University of Minho, 4710-057 Braga, PortugalPlant growth promoting rhizobacteria (PGPR) are in increasing demand due to their role in promoting sustainable practices, not only in agriculture but also in forestry. Keeping in mind the future application of PGPR for increasing cork oak sustainability, the aim of this study was to find cork oak PGPR isolates with increased nutrient solubilisation traits, able to promote root morphological changes and/or antagonize cork oak bark phytopathogens. Soils from three cork oak forests with distinct bioclimates (humid, semi-humid and semi-arid) were used for isolating bacteria. From the 7634 colony-forming units, 323 bacterial isolates were biochemically assayed for PGPR traits (siderophores production, phosphate solubilizing and organic acids production), and 51 were found to display all these traits. These PGPR were able to induce root morphological changes on <i>Arabidopsis thaliana</i>, like suppression of primary root growth, increase of lateral roots or root hairs formation. However, the most proficient PGPR displayed specific ability in changing a single root morphological trait. This ability was related not only to bacterial genotype, but also with the environment where bacteria thrived and isolation temperature. Bacteria from semi-arid environments (mainly <i>Bacillus megaterium</i> isolates) could hold a promising tool to enhance plant development. Other isolates (<i>Serratia quinivorens</i> or <i>B. cereus</i>) could be further explored for biocontrol purposes.https://www.mdpi.com/2076-2607/9/9/1973plant growth promoting bacteriacork oakantagonismbiocontrol agent |
| spellingShingle | Francisca Reis Ana João Pereira Rui M. Tavares Paula Baptista Teresa Lino-Neto Cork Oak Forests Soil Bacteria: Potential for Sustainable Agroforest Production Microorganisms plant growth promoting bacteria cork oak antagonism biocontrol agent |
| title | Cork Oak Forests Soil Bacteria: Potential for Sustainable Agroforest Production |
| title_full | Cork Oak Forests Soil Bacteria: Potential for Sustainable Agroforest Production |
| title_fullStr | Cork Oak Forests Soil Bacteria: Potential for Sustainable Agroforest Production |
| title_full_unstemmed | Cork Oak Forests Soil Bacteria: Potential for Sustainable Agroforest Production |
| title_short | Cork Oak Forests Soil Bacteria: Potential for Sustainable Agroforest Production |
| title_sort | cork oak forests soil bacteria potential for sustainable agroforest production |
| topic | plant growth promoting bacteria cork oak antagonism biocontrol agent |
| url | https://www.mdpi.com/2076-2607/9/9/1973 |
| work_keys_str_mv | AT franciscareis corkoakforestssoilbacteriapotentialforsustainableagroforestproduction AT anajoaopereira corkoakforestssoilbacteriapotentialforsustainableagroforestproduction AT ruimtavares corkoakforestssoilbacteriapotentialforsustainableagroforestproduction AT paulabaptista corkoakforestssoilbacteriapotentialforsustainableagroforestproduction AT teresalinoneto corkoakforestssoilbacteriapotentialforsustainableagroforestproduction |