The Detection of <i>Yr</i> Genes in Xinjiang Wheat Cultivars Using Different Molecular Markers
Wheat stripe rust is a fungal disease caused by <i>Puccinia striiformis</i> f. sp. <i>Tritici</i> (<i>Pst</i>). It significantly impacts wheat yields in Xinjiang, China. Breeding and promoting disease-resistant cultivars carrying disease-resistance genes remains t...
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MDPI AG
2023-08-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/17/13372 |
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| author | Minghao Zhang Ainisai Saimi Qi Liu Zeyu Ma Jing Chen |
| author_facet | Minghao Zhang Ainisai Saimi Qi Liu Zeyu Ma Jing Chen |
| author_sort | Minghao Zhang |
| collection | DOAJ |
| description | Wheat stripe rust is a fungal disease caused by <i>Puccinia striiformis</i> f. sp. <i>Tritici</i> (<i>Pst</i>). It significantly impacts wheat yields in Xinjiang, China. Breeding and promoting disease-resistant cultivars carrying disease-resistance genes remains the most cost-effective strategy with which to control the disease. In this study, 17 molecular markers were used to identify <i>Yr5</i>, <i>Yr9</i>, <i>Yr10</i>, <i>Yr15</i>, <i>Yr17</i>, <i>Yr18</i>, <i>Yr26</i>, <i>Yr41</i>, <i>Yr44</i>, and <i>Yr50</i> in 82 wheat cultivars from Xinjiang. According to the differences in SNP loci, the KASP markers for <i>Yr30</i>, <i>Yr52</i>, <i>Yr78</i>, <i>Yr80</i>, and <i>Yr81</i> were designed and detected in the same set of 82 wheat cultivars. The results showed that there was a diverse distribution of <i>Yr</i> genes across all wheat cultivars in Xinjiang, and the detection rates of <i>Yr5</i>, <i>Yr15</i>, <i>Yr17</i>, <i>Yr26</i>, <i>Yr41</i>, and <i>Yr50</i> were the highest, ranging from 74.39% to 98.78%. In addition, <i>Yr5</i> and <i>Yr15</i> were prevalent in spring wheat cultivars, with detection rates of 100% and 97.56%, respectively. A substantial 85.37% of wheat cultivars carried at least six or more different combinations of <i>Yr</i> genes. The cultivar Xindong No.15 exhibited the remarkable presence of 11 targeted <i>Yr</i> genes. The pedigree analysis results showed that 33.33% of Xinjiang wheat cultivars shared similar parentage, potentially leading to a loss of resistance against <i>Pst</i>. The results clarified the <i>Yr</i> gene distribution of the Xinjiang wheat cultivars and screened out varieties with a high resistance against <i>Pst</i>. |
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| spelling | doaj.art-1432f77fe9da4872b8d5d051c6e87c6b2023-11-19T08:16:21ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-08-0124171337210.3390/ijms241713372The Detection of <i>Yr</i> Genes in Xinjiang Wheat Cultivars Using Different Molecular MarkersMinghao Zhang0Ainisai Saimi1Qi Liu2Zeyu Ma3Jing Chen4Key Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of the Xinjiang Uygur Autonomous Region, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, ChinaKey Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of the Xinjiang Uygur Autonomous Region, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, ChinaKey Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of the Xinjiang Uygur Autonomous Region, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, ChinaKey Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of the Xinjiang Uygur Autonomous Region, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, ChinaKey Laboratory of the Pest Monitoring and Safety Control of Crops and Forests of the Xinjiang Uygur Autonomous Region, College of Agronomy, Xinjiang Agricultural University, Urumqi 830052, ChinaWheat stripe rust is a fungal disease caused by <i>Puccinia striiformis</i> f. sp. <i>Tritici</i> (<i>Pst</i>). It significantly impacts wheat yields in Xinjiang, China. Breeding and promoting disease-resistant cultivars carrying disease-resistance genes remains the most cost-effective strategy with which to control the disease. In this study, 17 molecular markers were used to identify <i>Yr5</i>, <i>Yr9</i>, <i>Yr10</i>, <i>Yr15</i>, <i>Yr17</i>, <i>Yr18</i>, <i>Yr26</i>, <i>Yr41</i>, <i>Yr44</i>, and <i>Yr50</i> in 82 wheat cultivars from Xinjiang. According to the differences in SNP loci, the KASP markers for <i>Yr30</i>, <i>Yr52</i>, <i>Yr78</i>, <i>Yr80</i>, and <i>Yr81</i> were designed and detected in the same set of 82 wheat cultivars. The results showed that there was a diverse distribution of <i>Yr</i> genes across all wheat cultivars in Xinjiang, and the detection rates of <i>Yr5</i>, <i>Yr15</i>, <i>Yr17</i>, <i>Yr26</i>, <i>Yr41</i>, and <i>Yr50</i> were the highest, ranging from 74.39% to 98.78%. In addition, <i>Yr5</i> and <i>Yr15</i> were prevalent in spring wheat cultivars, with detection rates of 100% and 97.56%, respectively. A substantial 85.37% of wheat cultivars carried at least six or more different combinations of <i>Yr</i> genes. The cultivar Xindong No.15 exhibited the remarkable presence of 11 targeted <i>Yr</i> genes. The pedigree analysis results showed that 33.33% of Xinjiang wheat cultivars shared similar parentage, potentially leading to a loss of resistance against <i>Pst</i>. The results clarified the <i>Yr</i> gene distribution of the Xinjiang wheat cultivars and screened out varieties with a high resistance against <i>Pst</i>.https://www.mdpi.com/1422-0067/24/17/13372resistance breedingresistance gene detectionwheat stripe rustXinjiang wheat cultivars |
| spellingShingle | Minghao Zhang Ainisai Saimi Qi Liu Zeyu Ma Jing Chen The Detection of <i>Yr</i> Genes in Xinjiang Wheat Cultivars Using Different Molecular Markers International Journal of Molecular Sciences resistance breeding resistance gene detection wheat stripe rust Xinjiang wheat cultivars |
| title | The Detection of <i>Yr</i> Genes in Xinjiang Wheat Cultivars Using Different Molecular Markers |
| title_full | The Detection of <i>Yr</i> Genes in Xinjiang Wheat Cultivars Using Different Molecular Markers |
| title_fullStr | The Detection of <i>Yr</i> Genes in Xinjiang Wheat Cultivars Using Different Molecular Markers |
| title_full_unstemmed | The Detection of <i>Yr</i> Genes in Xinjiang Wheat Cultivars Using Different Molecular Markers |
| title_short | The Detection of <i>Yr</i> Genes in Xinjiang Wheat Cultivars Using Different Molecular Markers |
| title_sort | detection of i yr i genes in xinjiang wheat cultivars using different molecular markers |
| topic | resistance breeding resistance gene detection wheat stripe rust Xinjiang wheat cultivars |
| url | https://www.mdpi.com/1422-0067/24/17/13372 |
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