Targeted Deletion of the First Intron of the Wx b Allele via CRISPR/Cas9 Significantly Increases Grain Amylose Content in Rice

Targeted Deletion of the First Intron of the Wx b Allele via CRISPR/Cas9 Significantly Increases Grain Amylose Content in Rice

Abstract Background The rice Waxy (Wx) gene plays a major role in seed amylose synthesis and consequently controls grain amylose content. Wx gene expression is highly regulated at the post-transcriptional level. In particular, the GT/TT polymorphism at the 5′splicing site of its 1st intron greatly a...

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Bibliographic Details
Main Authors: Xingdan Liu, Qi Ding, Wenshu Wang, Yanling Pan, Chao Tan, Yingbo Qiu, Ya Chen, Hongjing Li, Yinlong Li, Naizhong Ye, Nian Xu, Xiao Wu, Rongjian Ye, Jianfeng Liu, Chonglie Ma
Format: Article
Language:English
Published: SpringerOpen 2022-01-01
Series:Rice
Subjects:
Wx gene
GBSSI
CRISPR/Cas9
Intron
Amylose content
Rice
Online Access:https://doi.org/10.1186/s12284-021-00548-y

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