Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (<i>Vigna vexillata</i>) Reveals Energy Conservation and Root Plasticity Controlling Waterlogging Tolerance
<i>Vigna vexillata</i> (zombi pea) is an underutilized legume crop considered to be a potential gene source in breeding for abiotic stress tolerance. This study focuses on the molecular characterization of mechanisms controlling waterlogging tolerance using two zombi pea varieties with c...
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MDPI AG
2019-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/8/8/264 |
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| author | Pimprapai Butsayawarapat Piyada Juntawong Ornusa Khamsuk Prakit Somta |
| author_facet | Pimprapai Butsayawarapat Piyada Juntawong Ornusa Khamsuk Prakit Somta |
| author_sort | Pimprapai Butsayawarapat |
| collection | DOAJ |
| description | <i>Vigna vexillata</i> (zombi pea) is an underutilized legume crop considered to be a potential gene source in breeding for abiotic stress tolerance. This study focuses on the molecular characterization of mechanisms controlling waterlogging tolerance using two zombi pea varieties with contrasting waterlogging tolerance. Morphological examination revealed that in contrast to the sensitive variety, the tolerant variety was able to grow, maintain chlorophyll, form lateral roots, and develop aerenchyma in hypocotyl and taproots under waterlogging. To find the mechanism controlling waterlogging tolerance in zombi pea, comparative transcriptome analysis was performed using roots subjected to short-term waterlogging. Functional analysis indicated that glycolysis and fermentative genes were strongly upregulated in the sensitive variety, but not in the tolerant one. In contrast, the genes involved in auxin-regulated lateral root initiation and formation were expressed only in the tolerant variety. In addition, cell wall modification, aquaporin, and peroxidase genes were highly induced in the tolerant variety under waterlogging. Our findings suggest that energy management and root plasticity play important roles in mitigating the impact of waterlogging in zombi pea. The basic knowledge obtained from this study can be used in the molecular breeding of waterlogging-tolerant legume crops in the future. |
| first_indexed | 2024-12-24T03:25:07Z |
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| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-12-24T03:25:07Z |
| publishDate | 2019-08-01 |
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| record_format | Article |
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| spelling | doaj.art-1435abf837714124b3317e33aab99e332022-12-21T17:17:22ZengMDPI AGPlants2223-77472019-08-018826410.3390/plants8080264plants8080264Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (<i>Vigna vexillata</i>) Reveals Energy Conservation and Root Plasticity Controlling Waterlogging TolerancePimprapai Butsayawarapat0Piyada Juntawong1Ornusa Khamsuk2Prakit Somta3Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandDepartment of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandDepartment of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, ThailandDepartment of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Nakhon Pathom 73140, Thailand<i>Vigna vexillata</i> (zombi pea) is an underutilized legume crop considered to be a potential gene source in breeding for abiotic stress tolerance. This study focuses on the molecular characterization of mechanisms controlling waterlogging tolerance using two zombi pea varieties with contrasting waterlogging tolerance. Morphological examination revealed that in contrast to the sensitive variety, the tolerant variety was able to grow, maintain chlorophyll, form lateral roots, and develop aerenchyma in hypocotyl and taproots under waterlogging. To find the mechanism controlling waterlogging tolerance in zombi pea, comparative transcriptome analysis was performed using roots subjected to short-term waterlogging. Functional analysis indicated that glycolysis and fermentative genes were strongly upregulated in the sensitive variety, but not in the tolerant one. In contrast, the genes involved in auxin-regulated lateral root initiation and formation were expressed only in the tolerant variety. In addition, cell wall modification, aquaporin, and peroxidase genes were highly induced in the tolerant variety under waterlogging. Our findings suggest that energy management and root plasticity play important roles in mitigating the impact of waterlogging in zombi pea. The basic knowledge obtained from this study can be used in the molecular breeding of waterlogging-tolerant legume crops in the future.https://www.mdpi.com/2223-7747/8/8/264<i>De novo</i> transcriptomelateral rootlegume<i>Vigna vexillata</i>waterlogging |
| spellingShingle | Pimprapai Butsayawarapat Piyada Juntawong Ornusa Khamsuk Prakit Somta Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (<i>Vigna vexillata</i>) Reveals Energy Conservation and Root Plasticity Controlling Waterlogging Tolerance Plants <i>De novo</i> transcriptome lateral root legume <i>Vigna vexillata</i> waterlogging |
| title | Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (<i>Vigna vexillata</i>) Reveals Energy Conservation and Root Plasticity Controlling Waterlogging Tolerance |
| title_full | Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (<i>Vigna vexillata</i>) Reveals Energy Conservation and Root Plasticity Controlling Waterlogging Tolerance |
| title_fullStr | Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (<i>Vigna vexillata</i>) Reveals Energy Conservation and Root Plasticity Controlling Waterlogging Tolerance |
| title_full_unstemmed | Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (<i>Vigna vexillata</i>) Reveals Energy Conservation and Root Plasticity Controlling Waterlogging Tolerance |
| title_short | Comparative Transcriptome Analysis of Waterlogging-Sensitive and Tolerant Zombi Pea (<i>Vigna vexillata</i>) Reveals Energy Conservation and Root Plasticity Controlling Waterlogging Tolerance |
| title_sort | comparative transcriptome analysis of waterlogging sensitive and tolerant zombi pea i vigna vexillata i reveals energy conservation and root plasticity controlling waterlogging tolerance |
| topic | <i>De novo</i> transcriptome lateral root legume <i>Vigna vexillata</i> waterlogging |
| url | https://www.mdpi.com/2223-7747/8/8/264 |
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