Emersion and Relative Humidity Modulate Stress Response and Recovery Dynamics in Juvenile Mussels (<em>Perna canaliculus</em>)
The early stages of intertidal mussels, including the green-lipped mussel, <i>Perna canaliculus</i>, face both direct and indirect environmental threats. Stressors may influence physiological status and, ultimately, survival. An understanding of the nature of stress experienced is critic...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-08-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/11/9/580 |
| _version_ | 1797518192981049344 |
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| author | Natalí J. Delorme David J. Burritt Norman L. C. Ragg Paul M. South |
| author_facet | Natalí J. Delorme David J. Burritt Norman L. C. Ragg Paul M. South |
| author_sort | Natalí J. Delorme |
| collection | DOAJ |
| description | The early stages of intertidal mussels, including the green-lipped mussel, <i>Perna canaliculus</i>, face both direct and indirect environmental threats. Stressors may influence physiological status and, ultimately, survival. An understanding of the nature of stress experienced is critical to inform conservation and aquaculture efforts. Here, we investigated oxidative stress dynamics in juvenile <i>P. canaliculus</i> in relation to emersion duration (1–20 h) and relative humidity (RH, 29–98%) by quantifying oxidative damage (protein carbonyls, lipid hydroperoxides, 8-hydroxydeoxyguanosine) and enzymatic antioxidants (superoxide dismutase, catalase, glutathione peroxidase and reductase). Mussels held in low RH during emersion experienced severe water loss (>70%), high mortality (>80%) and increased oxidative damage (35–45% increase compared to control conditions), while mussels held at high RH were not impacted, even after 20 h of air exposure. Following re-immersion, reoxygenation stress resulted in further increases in damage markers in mussels that had experienced dryer emersion conditions; protective action of antioxidants increased steadily during the 10 h re-immersion period, apparently supporting a reduction in damage markers after 1–5 h of immersion. Clearly, conditions during emersion, as well as duration, substantially influence physiological performance and recovery of juvenile mussels. Successful recruitment to intertidal beds or survival in commercial aquaculture operations may be mediated by the nature of emersion stress experienced by these vulnerable juveniles. |
| first_indexed | 2024-03-10T07:26:43Z |
| format | Article |
| id | doaj.art-1436838d73174e568f80dd58968b1bca |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T07:26:43Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-1436838d73174e568f80dd58968b1bca2023-11-22T14:11:14ZengMDPI AGMetabolites2218-19892021-08-0111958010.3390/metabo11090580Emersion and Relative Humidity Modulate Stress Response and Recovery Dynamics in Juvenile Mussels (<em>Perna canaliculus</em>)Natalí J. Delorme0David J. Burritt1Norman L. C. Ragg2Paul M. South3Cawthron Institute, Private Bag 2, Nelson 7042, New ZealandDepartment of Botany, University of Otago, P.O. Box 56, Dunedin 9054, New ZealandCawthron Institute, Private Bag 2, Nelson 7042, New ZealandCawthron Institute, Private Bag 2, Nelson 7042, New ZealandThe early stages of intertidal mussels, including the green-lipped mussel, <i>Perna canaliculus</i>, face both direct and indirect environmental threats. Stressors may influence physiological status and, ultimately, survival. An understanding of the nature of stress experienced is critical to inform conservation and aquaculture efforts. Here, we investigated oxidative stress dynamics in juvenile <i>P. canaliculus</i> in relation to emersion duration (1–20 h) and relative humidity (RH, 29–98%) by quantifying oxidative damage (protein carbonyls, lipid hydroperoxides, 8-hydroxydeoxyguanosine) and enzymatic antioxidants (superoxide dismutase, catalase, glutathione peroxidase and reductase). Mussels held in low RH during emersion experienced severe water loss (>70%), high mortality (>80%) and increased oxidative damage (35–45% increase compared to control conditions), while mussels held at high RH were not impacted, even after 20 h of air exposure. Following re-immersion, reoxygenation stress resulted in further increases in damage markers in mussels that had experienced dryer emersion conditions; protective action of antioxidants increased steadily during the 10 h re-immersion period, apparently supporting a reduction in damage markers after 1–5 h of immersion. Clearly, conditions during emersion, as well as duration, substantially influence physiological performance and recovery of juvenile mussels. Successful recruitment to intertidal beds or survival in commercial aquaculture operations may be mediated by the nature of emersion stress experienced by these vulnerable juveniles.https://www.mdpi.com/2218-1989/11/9/580green-lipped musselGreenshell™ mussel<i>Perna canaliculus</i>spatemersionoxidative stress |
| spellingShingle | Natalí J. Delorme David J. Burritt Norman L. C. Ragg Paul M. South Emersion and Relative Humidity Modulate Stress Response and Recovery Dynamics in Juvenile Mussels (<em>Perna canaliculus</em>) Metabolites green-lipped mussel Greenshell™ mussel <i>Perna canaliculus</i> spat emersion oxidative stress |
| title | Emersion and Relative Humidity Modulate Stress Response and Recovery Dynamics in Juvenile Mussels (<em>Perna canaliculus</em>) |
| title_full | Emersion and Relative Humidity Modulate Stress Response and Recovery Dynamics in Juvenile Mussels (<em>Perna canaliculus</em>) |
| title_fullStr | Emersion and Relative Humidity Modulate Stress Response and Recovery Dynamics in Juvenile Mussels (<em>Perna canaliculus</em>) |
| title_full_unstemmed | Emersion and Relative Humidity Modulate Stress Response and Recovery Dynamics in Juvenile Mussels (<em>Perna canaliculus</em>) |
| title_short | Emersion and Relative Humidity Modulate Stress Response and Recovery Dynamics in Juvenile Mussels (<em>Perna canaliculus</em>) |
| title_sort | emersion and relative humidity modulate stress response and recovery dynamics in juvenile mussels em perna canaliculus em |
| topic | green-lipped mussel Greenshell™ mussel <i>Perna canaliculus</i> spat emersion oxidative stress |
| url | https://www.mdpi.com/2218-1989/11/9/580 |
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