Daphnetin alleviates neuropathic pain in chronic constrictive injury rats via regulating the NF-κB dependent CXCL1/CXCR2 signaling pathway
AbstractContext Daphnetin is a natural product with anti-inflammatory, antioxidant, and neuroprotective properties. Reports have found that it has a strong analgesic effect; however, its analgesic mechanism is unknown.Objective We explored the effect and mechanism of daphnetin on neuropathic pain (N...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
|
| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13880209.2023.2198560 |
| _version_ | 1827302060268191744 |
|---|---|
| author | Tianrui Zhang Wulin Liang Wenjing Ou Mingqian Zhang Shuang Cui Shuofeng Zhang |
| author_facet | Tianrui Zhang Wulin Liang Wenjing Ou Mingqian Zhang Shuang Cui Shuofeng Zhang |
| author_sort | Tianrui Zhang |
| collection | DOAJ |
| description | AbstractContext Daphnetin is a natural product with anti-inflammatory, antioxidant, and neuroprotective properties. Reports have found that it has a strong analgesic effect; however, its analgesic mechanism is unknown.Objective We explored the effect and mechanism of daphnetin on neuropathic pain (NP).Materials and methods The rat model of NP was established by ligation of the sciatic nerve. Male Sprague-Dawley rats were divided into six groups: Control, Model, Sham, morphine (0.375 mg/kg), and daphnetin (0.0625 and 0.025 mg/kg). Rats were intrathecally injected with drugs or normal saline once daily for three days. Hyperalgesia was evaluated by mechanical withdrawal threshold (MWT) and thermal withdrawal threshold (TWT). Protein levels were detected using ELISA, immunofluorescence, and western blotting.Results Compared to the Model group, daphnetin improved TWT (46.70 °C vs. 42.20 °C) and MWT (45.60 g vs. 23.60 g), reduced the expression of interleukin-1β (0.99 ng/g vs. 1.42 ng/g), interleukin-6 (0.90 ng/g vs. 1.52 ng/g), and tumor necrosis factor-α (0.93 ng/g vs. 1.52 ng/g) in the sciatic nerve. Daphnetin decreased the expression of toll-like receptor 4 (TLR4) (0.47-fold), phosphorylated inhibitor of NF-κB (p-IKBα) (0.29-fold), nuclear factor kappaB (NF-κB) (0.48-fold), glial fibrillary acidic protein (GFAP) (0.42-fold), CXC chemokine ligand type 1 (CXCL1) (0.84-fold), CXC chemokine receptor type 2 (CXCR2) (0.78-fold) in the spinal cord.Discussion and conclusions Daphnetin alleviates NP by inhibiting inflammation and astrocyte activation in the spinal cord, providing theoretical support for the extensive clinical treatment of NP. |
| first_indexed | 2024-03-09T00:08:49Z |
| format | Article |
| id | doaj.art-14369220e5b74db6b5e8d27682e8d6d1 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-04-24T16:38:01Z |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-14369220e5b74db6b5e8d27682e8d6d12024-03-29T11:10:26ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162023-12-0161174675410.1080/13880209.2023.2198560Daphnetin alleviates neuropathic pain in chronic constrictive injury rats via regulating the NF-κB dependent CXCL1/CXCR2 signaling pathwayTianrui Zhang0Wulin Liang1Wenjing Ou2Mingqian Zhang3Shuang Cui4Shuofeng Zhang5Department of Pharmacology of Traditional Chinese Medicine, College of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Pharmacology of Traditional Chinese Medicine, College of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Pharmacology of Traditional Chinese Medicine, College of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Pharmacology of Traditional Chinese Medicine, College of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Pharmacology of Traditional Chinese Medicine, College of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, ChinaDepartment of Pharmacology of Traditional Chinese Medicine, College of Chinese Medicine, Beijing University of Chinese Medicine, Beijing, ChinaAbstractContext Daphnetin is a natural product with anti-inflammatory, antioxidant, and neuroprotective properties. Reports have found that it has a strong analgesic effect; however, its analgesic mechanism is unknown.Objective We explored the effect and mechanism of daphnetin on neuropathic pain (NP).Materials and methods The rat model of NP was established by ligation of the sciatic nerve. Male Sprague-Dawley rats were divided into six groups: Control, Model, Sham, morphine (0.375 mg/kg), and daphnetin (0.0625 and 0.025 mg/kg). Rats were intrathecally injected with drugs or normal saline once daily for three days. Hyperalgesia was evaluated by mechanical withdrawal threshold (MWT) and thermal withdrawal threshold (TWT). Protein levels were detected using ELISA, immunofluorescence, and western blotting.Results Compared to the Model group, daphnetin improved TWT (46.70 °C vs. 42.20 °C) and MWT (45.60 g vs. 23.60 g), reduced the expression of interleukin-1β (0.99 ng/g vs. 1.42 ng/g), interleukin-6 (0.90 ng/g vs. 1.52 ng/g), and tumor necrosis factor-α (0.93 ng/g vs. 1.52 ng/g) in the sciatic nerve. Daphnetin decreased the expression of toll-like receptor 4 (TLR4) (0.47-fold), phosphorylated inhibitor of NF-κB (p-IKBα) (0.29-fold), nuclear factor kappaB (NF-κB) (0.48-fold), glial fibrillary acidic protein (GFAP) (0.42-fold), CXC chemokine ligand type 1 (CXCL1) (0.84-fold), CXC chemokine receptor type 2 (CXCR2) (0.78-fold) in the spinal cord.Discussion and conclusions Daphnetin alleviates NP by inhibiting inflammation and astrocyte activation in the spinal cord, providing theoretical support for the extensive clinical treatment of NP.https://www.tandfonline.com/doi/10.1080/13880209.2023.2198560Neuralgiadaphnetinastrocytescrosstalkchemokine |
| spellingShingle | Tianrui Zhang Wulin Liang Wenjing Ou Mingqian Zhang Shuang Cui Shuofeng Zhang Daphnetin alleviates neuropathic pain in chronic constrictive injury rats via regulating the NF-κB dependent CXCL1/CXCR2 signaling pathway Pharmaceutical Biology Neuralgia daphnetin astrocytes crosstalk chemokine |
| title | Daphnetin alleviates neuropathic pain in chronic constrictive injury rats via regulating the NF-κB dependent CXCL1/CXCR2 signaling pathway |
| title_full | Daphnetin alleviates neuropathic pain in chronic constrictive injury rats via regulating the NF-κB dependent CXCL1/CXCR2 signaling pathway |
| title_fullStr | Daphnetin alleviates neuropathic pain in chronic constrictive injury rats via regulating the NF-κB dependent CXCL1/CXCR2 signaling pathway |
| title_full_unstemmed | Daphnetin alleviates neuropathic pain in chronic constrictive injury rats via regulating the NF-κB dependent CXCL1/CXCR2 signaling pathway |
| title_short | Daphnetin alleviates neuropathic pain in chronic constrictive injury rats via regulating the NF-κB dependent CXCL1/CXCR2 signaling pathway |
| title_sort | daphnetin alleviates neuropathic pain in chronic constrictive injury rats via regulating the nf κb dependent cxcl1 cxcr2 signaling pathway |
| topic | Neuralgia daphnetin astrocytes crosstalk chemokine |
| url | https://www.tandfonline.com/doi/10.1080/13880209.2023.2198560 |
| work_keys_str_mv | AT tianruizhang daphnetinalleviatesneuropathicpaininchronicconstrictiveinjuryratsviaregulatingthenfkbdependentcxcl1cxcr2signalingpathway AT wulinliang daphnetinalleviatesneuropathicpaininchronicconstrictiveinjuryratsviaregulatingthenfkbdependentcxcl1cxcr2signalingpathway AT wenjingou daphnetinalleviatesneuropathicpaininchronicconstrictiveinjuryratsviaregulatingthenfkbdependentcxcl1cxcr2signalingpathway AT mingqianzhang daphnetinalleviatesneuropathicpaininchronicconstrictiveinjuryratsviaregulatingthenfkbdependentcxcl1cxcr2signalingpathway AT shuangcui daphnetinalleviatesneuropathicpaininchronicconstrictiveinjuryratsviaregulatingthenfkbdependentcxcl1cxcr2signalingpathway AT shuofengzhang daphnetinalleviatesneuropathicpaininchronicconstrictiveinjuryratsviaregulatingthenfkbdependentcxcl1cxcr2signalingpathway |