Dynamic Changes of Rhizosphere Soil Microbiome and Functional Genes Involved in Carbon and Nitrogen Cycling in Chinese Fir Monoculture
We used metagenomics to investigate the rhizosphere microbial community assembly and functions associated with different nutrient cycles in Chinese fir at different monoculture times and growth stages. Mantel test results indicated significant positive correlations between soil TP contents and bacte...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Forests |
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| Online Access: | https://www.mdpi.com/1999-4907/13/11/1906 |
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| author | Shuzhen Wang Wenwen Chen Qianqian Gao Chuifan Zhou |
| author_facet | Shuzhen Wang Wenwen Chen Qianqian Gao Chuifan Zhou |
| author_sort | Shuzhen Wang |
| collection | DOAJ |
| description | We used metagenomics to investigate the rhizosphere microbial community assembly and functions associated with different nutrient cycles in Chinese fir at different monoculture times and growth stages. Mantel test results indicated significant positive correlations between soil TP contents and bacterial communities. The concentrations of soil AP also exhibited a significantly positive association with the fungal community. The relative abundance of ko00720 and ko00680 increased from young-old stands to mature stands. It then decreased in over-mature plantations (45 years) and had a recovery in 102-year-old stands. The potential degradation pathway of cellulose had the highest abundance in 26-year-old stands than the other aged plantations. Potential N cycling processes were dominated by assimilatory nitrate reduction to ammonium and dissimilatory nitrate reduction to ammonium pathways. The variation-partitioning analysis revealed that three forms of N contents (NH<sub>4</sub><sup>+</sup>-N, NO<sub>3</sub><sup>−</sup>-N, and DON) comprised 7%, whereas the other soil properties constituted 15.6% variation in the relative abundance of the genes involved in N cycling. Thus, metagenomics elucidates the evolution characteristics of rhizomicrobial composition and their functional changes at different developmental stages of Chinese fir plantations, providing a suitable reference for the potential utilization of carbon and nitrogen properties. |
| first_indexed | 2024-03-09T18:19:17Z |
| format | Article |
| id | doaj.art-1437407ec97742c3b8c292b307e1a0c5 |
| institution | Directory Open Access Journal |
| issn | 1999-4907 |
| language | English |
| last_indexed | 2024-03-09T18:19:17Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
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| spelling | doaj.art-1437407ec97742c3b8c292b307e1a0c52023-11-24T08:23:52ZengMDPI AGForests1999-49072022-11-011311190610.3390/f13111906Dynamic Changes of Rhizosphere Soil Microbiome and Functional Genes Involved in Carbon and Nitrogen Cycling in Chinese Fir MonocultureShuzhen Wang0Wenwen Chen1Qianqian Gao2Chuifan Zhou3Forestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaForestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaForestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaForestry College, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaWe used metagenomics to investigate the rhizosphere microbial community assembly and functions associated with different nutrient cycles in Chinese fir at different monoculture times and growth stages. Mantel test results indicated significant positive correlations between soil TP contents and bacterial communities. The concentrations of soil AP also exhibited a significantly positive association with the fungal community. The relative abundance of ko00720 and ko00680 increased from young-old stands to mature stands. It then decreased in over-mature plantations (45 years) and had a recovery in 102-year-old stands. The potential degradation pathway of cellulose had the highest abundance in 26-year-old stands than the other aged plantations. Potential N cycling processes were dominated by assimilatory nitrate reduction to ammonium and dissimilatory nitrate reduction to ammonium pathways. The variation-partitioning analysis revealed that three forms of N contents (NH<sub>4</sub><sup>+</sup>-N, NO<sub>3</sub><sup>−</sup>-N, and DON) comprised 7%, whereas the other soil properties constituted 15.6% variation in the relative abundance of the genes involved in N cycling. Thus, metagenomics elucidates the evolution characteristics of rhizomicrobial composition and their functional changes at different developmental stages of Chinese fir plantations, providing a suitable reference for the potential utilization of carbon and nitrogen properties.https://www.mdpi.com/1999-4907/13/11/1906Chinese fir plantationsrhizospheremonoculturemetagenomefunctional geneC and N cycling |
| spellingShingle | Shuzhen Wang Wenwen Chen Qianqian Gao Chuifan Zhou Dynamic Changes of Rhizosphere Soil Microbiome and Functional Genes Involved in Carbon and Nitrogen Cycling in Chinese Fir Monoculture Forests Chinese fir plantations rhizosphere monoculture metagenome functional gene C and N cycling |
| title | Dynamic Changes of Rhizosphere Soil Microbiome and Functional Genes Involved in Carbon and Nitrogen Cycling in Chinese Fir Monoculture |
| title_full | Dynamic Changes of Rhizosphere Soil Microbiome and Functional Genes Involved in Carbon and Nitrogen Cycling in Chinese Fir Monoculture |
| title_fullStr | Dynamic Changes of Rhizosphere Soil Microbiome and Functional Genes Involved in Carbon and Nitrogen Cycling in Chinese Fir Monoculture |
| title_full_unstemmed | Dynamic Changes of Rhizosphere Soil Microbiome and Functional Genes Involved in Carbon and Nitrogen Cycling in Chinese Fir Monoculture |
| title_short | Dynamic Changes of Rhizosphere Soil Microbiome and Functional Genes Involved in Carbon and Nitrogen Cycling in Chinese Fir Monoculture |
| title_sort | dynamic changes of rhizosphere soil microbiome and functional genes involved in carbon and nitrogen cycling in chinese fir monoculture |
| topic | Chinese fir plantations rhizosphere monoculture metagenome functional gene C and N cycling |
| url | https://www.mdpi.com/1999-4907/13/11/1906 |
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