Skeletal Muscle Signaling Following Whole-Body and Localized Heat Exposure in Humans
This study identified the changes in hypertrophy/atrophy and mitochondrial-related signaling in human skeletal muscle following whole-body (WB) and localized single leg (SL) heat treatment. Nine active male participants were administered either 60 min of passive WB (44–50°C, 50% humidity) or SL (wat...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2020.00839/full |
| _version_ | 1818684590611496960 |
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| author | Mohammed Ihsan Louise Deldicque John Molphy Florian Britto Anissa Cherif Sebastien Racinais |
| author_facet | Mohammed Ihsan Louise Deldicque John Molphy Florian Britto Anissa Cherif Sebastien Racinais |
| author_sort | Mohammed Ihsan |
| collection | DOAJ |
| description | This study identified the changes in hypertrophy/atrophy and mitochondrial-related signaling in human skeletal muscle following whole-body (WB) and localized single leg (SL) heat treatment. Nine active male participants were administered either 60 min of passive WB (44–50°C, 50% humidity) or SL (water-perfused suit at 49.5 ± 1.4°C) heat treatment at least 1 week apart in a counterbalanced order. The untreated leg during SL was considered as control (CON). Core, skin, and quadriceps muscle temperature were monitored throughout the experimental trials. Muscle microbiopsy samples were obtained prior to (PRE), and 30 min and 3 h post (POST) following heat treatment. Muscle temperature increased with time (p < 0.0001) in both WB and SL, with no differences between conditions (38.8 ± 0.5°C vs. 38.1 ± 0.6°C, p = 0.065). Core temperature increased only following WB, and was significantly higher compared with SL (39.1 ± 0.3°C vs. 37.1 ± 0.1, p < 0.0001). Compared with PRE, WB up-regulated the phosphorylation status of the majority of the Akt/mTOR pathway (Akt, mTOR, S6K1, rpS6, and p-eIF4E; p ≤ 0.050), with the exception of 4EBP1 (p = 0.139). WB also increased the mRNA of HSPs 72, 90, and 25 (all p < 0.021), and increased or tended to increase the phosphorylation of FOXO1 (p = 0.066) and FOXO3a (p = 0.038). In addition, most (NRF1, NRF2, COX2, and COX4-I2; all p ≤ 0.050), but not all (CS, Cyt c, and COX4-I1; p > 0.441) mRNA content indicative of mitochondrial biogenesis were increased following WB, with no changes evident in these parameters in SL or CON (all p > 0.090). These results indicate that 1 h of WB heat treatment enhanced anabolic (Akt/mTOR), mitochondrial, and cyto-protective signaling (HSP), with a concomitant possible inhibition of FOXO transcription factors. |
| first_indexed | 2024-12-17T10:53:03Z |
| format | Article |
| id | doaj.art-143d71481efd4e4c9c905b50a53d758c |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-17T10:53:03Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-143d71481efd4e4c9c905b50a53d758c2022-12-21T21:51:54ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2020-07-011110.3389/fphys.2020.00839535470Skeletal Muscle Signaling Following Whole-Body and Localized Heat Exposure in HumansMohammed Ihsan0Louise Deldicque1John Molphy2Florian Britto3Anissa Cherif4Sebastien Racinais5Research and Scientific Support, Aspetar Orthopaedic and Sports Medicine Hospital, Doha, QatarInstitute of Neuroscience, Université catholique de Louvain, Louvain-la-Neuve, BelgiumResearch and Scientific Support, Aspetar Orthopaedic and Sports Medicine Hospital, Doha, QatarInstitute of Neuroscience, Université catholique de Louvain, Louvain-la-Neuve, BelgiumResearch and Scientific Support, Aspetar Orthopaedic and Sports Medicine Hospital, Doha, QatarResearch and Scientific Support, Aspetar Orthopaedic and Sports Medicine Hospital, Doha, QatarThis study identified the changes in hypertrophy/atrophy and mitochondrial-related signaling in human skeletal muscle following whole-body (WB) and localized single leg (SL) heat treatment. Nine active male participants were administered either 60 min of passive WB (44–50°C, 50% humidity) or SL (water-perfused suit at 49.5 ± 1.4°C) heat treatment at least 1 week apart in a counterbalanced order. The untreated leg during SL was considered as control (CON). Core, skin, and quadriceps muscle temperature were monitored throughout the experimental trials. Muscle microbiopsy samples were obtained prior to (PRE), and 30 min and 3 h post (POST) following heat treatment. Muscle temperature increased with time (p < 0.0001) in both WB and SL, with no differences between conditions (38.8 ± 0.5°C vs. 38.1 ± 0.6°C, p = 0.065). Core temperature increased only following WB, and was significantly higher compared with SL (39.1 ± 0.3°C vs. 37.1 ± 0.1, p < 0.0001). Compared with PRE, WB up-regulated the phosphorylation status of the majority of the Akt/mTOR pathway (Akt, mTOR, S6K1, rpS6, and p-eIF4E; p ≤ 0.050), with the exception of 4EBP1 (p = 0.139). WB also increased the mRNA of HSPs 72, 90, and 25 (all p < 0.021), and increased or tended to increase the phosphorylation of FOXO1 (p = 0.066) and FOXO3a (p = 0.038). In addition, most (NRF1, NRF2, COX2, and COX4-I2; all p ≤ 0.050), but not all (CS, Cyt c, and COX4-I1; p > 0.441) mRNA content indicative of mitochondrial biogenesis were increased following WB, with no changes evident in these parameters in SL or CON (all p > 0.090). These results indicate that 1 h of WB heat treatment enhanced anabolic (Akt/mTOR), mitochondrial, and cyto-protective signaling (HSP), with a concomitant possible inhibition of FOXO transcription factors.https://www.frontiersin.org/article/10.3389/fphys.2020.00839/fullmuscle massheat treatmenthypertrophymitochondrial biogenesisheat shock proteinmuscle atrophy |
| spellingShingle | Mohammed Ihsan Louise Deldicque John Molphy Florian Britto Anissa Cherif Sebastien Racinais Skeletal Muscle Signaling Following Whole-Body and Localized Heat Exposure in Humans Frontiers in Physiology muscle mass heat treatment hypertrophy mitochondrial biogenesis heat shock protein muscle atrophy |
| title | Skeletal Muscle Signaling Following Whole-Body and Localized Heat Exposure in Humans |
| title_full | Skeletal Muscle Signaling Following Whole-Body and Localized Heat Exposure in Humans |
| title_fullStr | Skeletal Muscle Signaling Following Whole-Body and Localized Heat Exposure in Humans |
| title_full_unstemmed | Skeletal Muscle Signaling Following Whole-Body and Localized Heat Exposure in Humans |
| title_short | Skeletal Muscle Signaling Following Whole-Body and Localized Heat Exposure in Humans |
| title_sort | skeletal muscle signaling following whole body and localized heat exposure in humans |
| topic | muscle mass heat treatment hypertrophy mitochondrial biogenesis heat shock protein muscle atrophy |
| url | https://www.frontiersin.org/article/10.3389/fphys.2020.00839/full |
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