The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells
Ovarian granulosa cells (GCs) are a critical approach to investigate the mechanism of gene regulation during folliculogenesis. The objective of this study was to investigate the role of MT2 in bovine GCs, and assess whether MT2 silencing affected GCs response to melatonin. We found that MT2 silencin...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-07-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1422-0067/19/7/2028 |
| _version_ | 1811281635911401472 |
|---|---|
| author | Shujuan Wang Wenju Liu Xunsheng Pang Sifa Dai Guodong Liu |
| author_facet | Shujuan Wang Wenju Liu Xunsheng Pang Sifa Dai Guodong Liu |
| author_sort | Shujuan Wang |
| collection | DOAJ |
| description | Ovarian granulosa cells (GCs) are a critical approach to investigate the mechanism of gene regulation during folliculogenesis. The objective of this study was to investigate the role of MT2 in bovine GCs, and assess whether MT2 silencing affected GCs response to melatonin. We found that MT2 silencing significantly decreased the secretion of progesterone and estradiol, and increased the concentration of inhibin B and activin B. To further reveal the regulatory mechanism of MT2 silencing on steroids synthesis, it was found that the expression of CYP19A1 and CYP11A1 enzymes (steroid hormone synthesis) were down-regulated, while genes related to hormonal synthesis (StAR, RUNX2, INHA and INHBB) were up-regulated without affecting the expression of INHBA, suggesting that MT2 silencing may regulate hormone abundance. Furthermore, MT2 silencing significantly increased the expression of TGFBR3 and BMP6, and decreased the expression of LHR and DNMT1A without significant difference in the expression of FSHR and EGFR. In addition, MT2 silencing didn’t affect the effect of melatonin on increasing the expression of DNMT1A, EGFR, INHBA and LHR, and progesterone level, or decreasing INHA, TGFBR3 and StAR expression, and production of inhibin B. Moreover, MT2 silencing could disrupt the role of melatonin in decreasing the FSHR, INHBB and BMP6 expression, and activin B secretion. In conclusion, these results reveal that melatonin and MT2 are essential regulator of bovine GCs function by modulating reproduction-related genes expression, hormones secretion and other regulators of folliculogenesis. |
| first_indexed | 2024-04-13T01:36:04Z |
| format | Article |
| id | doaj.art-143e231eda174c3285bd87fd639b2e63 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T01:36:04Z |
| publishDate | 2018-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-143e231eda174c3285bd87fd639b2e632022-12-22T03:08:20ZengMDPI AGInternational Journal of Molecular Sciences1422-00672018-07-01197202810.3390/ijms19072028ijms19072028The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa CellsShujuan Wang0Wenju Liu1Xunsheng Pang2Sifa Dai3Guodong Liu4College of Animal Science, Anhui Science and Technology University, Fengyang 233100, ChinaResearch Center for Biomedical and Health Science, Anhui Science and Technology University, Fengyang 233100, ChinaCollege of Animal Science, Anhui Science and Technology University, Fengyang 233100, ChinaCollege of Animal Science, Anhui Science and Technology University, Fengyang 233100, ChinaResearch Center for Biomedical and Health Science, Anhui Science and Technology University, Fengyang 233100, ChinaOvarian granulosa cells (GCs) are a critical approach to investigate the mechanism of gene regulation during folliculogenesis. The objective of this study was to investigate the role of MT2 in bovine GCs, and assess whether MT2 silencing affected GCs response to melatonin. We found that MT2 silencing significantly decreased the secretion of progesterone and estradiol, and increased the concentration of inhibin B and activin B. To further reveal the regulatory mechanism of MT2 silencing on steroids synthesis, it was found that the expression of CYP19A1 and CYP11A1 enzymes (steroid hormone synthesis) were down-regulated, while genes related to hormonal synthesis (StAR, RUNX2, INHA and INHBB) were up-regulated without affecting the expression of INHBA, suggesting that MT2 silencing may regulate hormone abundance. Furthermore, MT2 silencing significantly increased the expression of TGFBR3 and BMP6, and decreased the expression of LHR and DNMT1A without significant difference in the expression of FSHR and EGFR. In addition, MT2 silencing didn’t affect the effect of melatonin on increasing the expression of DNMT1A, EGFR, INHBA and LHR, and progesterone level, or decreasing INHA, TGFBR3 and StAR expression, and production of inhibin B. Moreover, MT2 silencing could disrupt the role of melatonin in decreasing the FSHR, INHBB and BMP6 expression, and activin B secretion. In conclusion, these results reveal that melatonin and MT2 are essential regulator of bovine GCs function by modulating reproduction-related genes expression, hormones secretion and other regulators of folliculogenesis.http://www.mdpi.com/1422-0067/19/7/2028RNA interferenceshRNAmelatoningranulosa cellMT2hormonegene regulation |
| spellingShingle | Shujuan Wang Wenju Liu Xunsheng Pang Sifa Dai Guodong Liu The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells International Journal of Molecular Sciences RNA interference shRNA melatonin granulosa cell MT2 hormone gene regulation |
| title | The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells |
| title_full | The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells |
| title_fullStr | The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells |
| title_full_unstemmed | The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells |
| title_short | The Mechanism of Melatonin and Its Receptor MT2 Involved in the Development of Bovine Granulosa Cells |
| title_sort | mechanism of melatonin and its receptor mt2 involved in the development of bovine granulosa cells |
| topic | RNA interference shRNA melatonin granulosa cell MT2 hormone gene regulation |
| url | http://www.mdpi.com/1422-0067/19/7/2028 |
| work_keys_str_mv | AT shujuanwang themechanismofmelatoninanditsreceptormt2involvedinthedevelopmentofbovinegranulosacells AT wenjuliu themechanismofmelatoninanditsreceptormt2involvedinthedevelopmentofbovinegranulosacells AT xunshengpang themechanismofmelatoninanditsreceptormt2involvedinthedevelopmentofbovinegranulosacells AT sifadai themechanismofmelatoninanditsreceptormt2involvedinthedevelopmentofbovinegranulosacells AT guodongliu themechanismofmelatoninanditsreceptormt2involvedinthedevelopmentofbovinegranulosacells AT shujuanwang mechanismofmelatoninanditsreceptormt2involvedinthedevelopmentofbovinegranulosacells AT wenjuliu mechanismofmelatoninanditsreceptormt2involvedinthedevelopmentofbovinegranulosacells AT xunshengpang mechanismofmelatoninanditsreceptormt2involvedinthedevelopmentofbovinegranulosacells AT sifadai mechanismofmelatoninanditsreceptormt2involvedinthedevelopmentofbovinegranulosacells AT guodongliu mechanismofmelatoninanditsreceptormt2involvedinthedevelopmentofbovinegranulosacells |