Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant—Alzheimer's disease (ASUi005-A) and healthy non-demented control (ASUi006-A)
Although the majority of late-onset Alzheimer's disease (AD) patients are labeled sporadic, multiple genetic risk variants have been identified, the most powerful and prevalent of which is the e4 variant of the Apolipoprotein E (APOE) gene. Here, we generated human induced pluripotent stem cell...
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| Format: | Article |
| Language: | English |
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Elsevier
2018-10-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506118302277 |
| _version_ | 1811212855034249216 |
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| author | Nicholas Brookhouser Ping Zhang Richard Caselli Jean J. Kim David A. Brafman |
| author_facet | Nicholas Brookhouser Ping Zhang Richard Caselli Jean J. Kim David A. Brafman |
| author_sort | Nicholas Brookhouser |
| collection | DOAJ |
| description | Although the majority of late-onset Alzheimer's disease (AD) patients are labeled sporadic, multiple genetic risk variants have been identified, the most powerful and prevalent of which is the e4 variant of the Apolipoprotein E (APOE) gene. Here, we generated human induced pluripotent stem cell (hiPSC) lines from the peripheral blood mononuclear cells (PBMCs) of a clinically diagnosed AD patient [ASUi005-A] and a non-demented control (NDC) patient [ASUi006-A] homozygous for the APOE4 risk allele. These hiPSCs maintained their original genotype, expressed pluripotency markers, exhibited a normal karyotype, and retained the ability to differentiate into cells representative of the three germ layers.Resource tableUnlabelled TableUnique stem cell lines identifierASUi005-AASUi006-AAlternative names of stem cell linesASU-161ASU-487InstitutionArizona State University; Tempe, AZ; USAContact information of distributorDavid Brafman, David.Brafman@asu.eduType of cell linesiPSCOriginHumanCell sourceHuman peripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingCytoTune®-iPS 2.0 Reprogramming SystemMultiline rationaleAge-matched Alzheimer's disease and non-demented control hiPSC lines homozygous for the APOE 4 risk factorGene modificationNoType of modificationN/AAssociated diseaseAlzheimer's diseaseGene/locusApolipoprotein E (APOE)Method of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateMarch 2018Cell line repository/bankNot applicableEthical approvalMayo Clinic Institutional Review Board; IRB # 15-008674 |
| first_indexed | 2024-04-12T05:37:27Z |
| format | Article |
| id | doaj.art-1444ca730b154d22b21c99e031e5e39c |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-12T05:37:27Z |
| publishDate | 2018-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-1444ca730b154d22b21c99e031e5e39c2022-12-22T03:45:48ZengElsevierStem Cell Research1873-50612018-10-0132145149Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant—Alzheimer's disease (ASUi005-A) and healthy non-demented control (ASUi006-A)Nicholas Brookhouser0Ping Zhang1Richard Caselli2Jean J. Kim3David A. Brafman4School of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USAMolecular and Cellular Biology, Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, TX 77030, USADepartment of Neurology, Mayo Clinic College of Medicine, Scottsdale, AZ 85259, USAMolecular and Cellular Biology, Stem Cells and Regenerative Medicine Center, Baylor College of Medicine, Houston, TX 77030, USASchool of Biological and Health Systems Engineering, Arizona State University, Tempe, AZ 85287, USA; Corresponding author.Although the majority of late-onset Alzheimer's disease (AD) patients are labeled sporadic, multiple genetic risk variants have been identified, the most powerful and prevalent of which is the e4 variant of the Apolipoprotein E (APOE) gene. Here, we generated human induced pluripotent stem cell (hiPSC) lines from the peripheral blood mononuclear cells (PBMCs) of a clinically diagnosed AD patient [ASUi005-A] and a non-demented control (NDC) patient [ASUi006-A] homozygous for the APOE4 risk allele. These hiPSCs maintained their original genotype, expressed pluripotency markers, exhibited a normal karyotype, and retained the ability to differentiate into cells representative of the three germ layers.Resource tableUnlabelled TableUnique stem cell lines identifierASUi005-AASUi006-AAlternative names of stem cell linesASU-161ASU-487InstitutionArizona State University; Tempe, AZ; USAContact information of distributorDavid Brafman, David.Brafman@asu.eduType of cell linesiPSCOriginHumanCell sourceHuman peripheral blood mononuclear cells (PBMCs)ClonalityClonalMethod of reprogrammingCytoTune®-iPS 2.0 Reprogramming SystemMultiline rationaleAge-matched Alzheimer's disease and non-demented control hiPSC lines homozygous for the APOE 4 risk factorGene modificationNoType of modificationN/AAssociated diseaseAlzheimer's diseaseGene/locusApolipoprotein E (APOE)Method of modificationN/AName of transgene or resistanceN/AInducible/constitutive systemN/ADate archived/stock dateMarch 2018Cell line repository/bankNot applicableEthical approvalMayo Clinic Institutional Review Board; IRB # 15-008674http://www.sciencedirect.com/science/article/pii/S1873506118302277 |
| spellingShingle | Nicholas Brookhouser Ping Zhang Richard Caselli Jean J. Kim David A. Brafman Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant—Alzheimer's disease (ASUi005-A) and healthy non-demented control (ASUi006-A) Stem Cell Research |
| title | Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant—Alzheimer's disease (ASUi005-A) and healthy non-demented control (ASUi006-A) |
| title_full | Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant—Alzheimer's disease (ASUi005-A) and healthy non-demented control (ASUi006-A) |
| title_fullStr | Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant—Alzheimer's disease (ASUi005-A) and healthy non-demented control (ASUi006-A) |
| title_full_unstemmed | Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant—Alzheimer's disease (ASUi005-A) and healthy non-demented control (ASUi006-A) |
| title_short | Generation and characterization of two human induced pluripotent stem cell (hiPSC) lines homozygous for the Apolipoprotein e4 (APOE4) risk variant—Alzheimer's disease (ASUi005-A) and healthy non-demented control (ASUi006-A) |
| title_sort | generation and characterization of two human induced pluripotent stem cell hipsc lines homozygous for the apolipoprotein e4 apoe4 risk variant alzheimer s disease asui005 a and healthy non demented control asui006 a |
| url | http://www.sciencedirect.com/science/article/pii/S1873506118302277 |
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