Solid State Fermentation of Shrimp Shell Waste Using <i>Pseudonocardia carboxydivorans</i> 18A13O1 to Produce Bioactive Metabolites
Marine actinomycetes are prolific microorganisms; however, knowledge of their diversity, distribution, and secondary metabolites is limited. Marine actinomycetes represent an untapped source of novel bioactive compounds. In this study, we investigated shrimp shell as substrates for model production...
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MDPI AG
2021-10-01
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author | Andi Setiawan Widyastuti Widyastuti Arik Irawan Oklis Syahrin Wijaya Aspita Laila Wawan Abdullah Setiawan Ni Luh Gede Ratna Juliasih Kenichi Nonaka Masayoshi Arai John Hendri |
author_facet | Andi Setiawan Widyastuti Widyastuti Arik Irawan Oklis Syahrin Wijaya Aspita Laila Wawan Abdullah Setiawan Ni Luh Gede Ratna Juliasih Kenichi Nonaka Masayoshi Arai John Hendri |
author_sort | Andi Setiawan |
collection | DOAJ |
description | Marine actinomycetes are prolific microorganisms; however, knowledge of their diversity, distribution, and secondary metabolites is limited. Marine actinomycetes represent an untapped source of novel bioactive compounds. In this study, we investigated shrimp shell as substrates for model production bioactive metabolites from actinomycetes under solid state fermentation (SSF) conditions. A total of fifteen actinomycetes were isolated from six sponges and one tunicate. The isolated actinomycetes were grown on solid shrimp shells. Cultures of actinomycetes were extracted with ethyl acetate (EtOAc) and extracts were bioassayed for activity against <i>Staphylococcus aureus</i>. One isolate 18A13O1 from the sponge, <i>Rhabdastrella globostellata</i>, exhibited antibacterial activity on primary screening compared to the other samples and was chosen for further study. Visualization using SEM showed aerial and substrate mycelia. Through phylogenetic analysis, it was confirmed that isolate 18A13O1 is a <i>Pseudonocardia carboxydivorans</i>. Purification of an EtOAc extract yielded A13B2, which showed a minimum inhibition concentration against <i>S. aureus</i> at 15.6 μg/mL. It can be concluded that this basic information is very important for further studies related to the development of the production of bioactive secondary metabolites through the solid state fermentation process. |
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issn | 2311-5637 |
language | English |
last_indexed | 2024-03-10T04:09:51Z |
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spelling | doaj.art-1448d8d962df494b91708eedfda61a962023-11-23T08:12:59ZengMDPI AGFermentation2311-56372021-10-017424710.3390/fermentation7040247Solid State Fermentation of Shrimp Shell Waste Using <i>Pseudonocardia carboxydivorans</i> 18A13O1 to Produce Bioactive MetabolitesAndi Setiawan0Widyastuti Widyastuti1Arik Irawan2Oklis Syahrin Wijaya3Aspita Laila4Wawan Abdullah Setiawan5Ni Luh Gede Ratna Juliasih6Kenichi Nonaka7Masayoshi Arai8John Hendri9Department of Chemistry, Faculty of Mathematics and Natural Sciences, Lampung University, Bandar Lampung 35145, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Lampung University, Bandar Lampung 35145, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Lampung University, Bandar Lampung 35145, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Lampung University, Bandar Lampung 35145, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Lampung University, Bandar Lampung 35145, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Science, Lampung University, Bandar Lampung 35145, IndonesiaDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Lampung University, Bandar Lampung 35145, IndonesiaŌmura Satoshi Memorial Institute, Kitasato University, 5-9-1 Shirokane Minato-ku, Tokyo 108-8641, JapanGraduate School of Pharmaceutical Sciences, Osaka University, 1-6 Yamada-oka, Suita 565-0871, JapanDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Lampung University, Bandar Lampung 35145, IndonesiaMarine actinomycetes are prolific microorganisms; however, knowledge of their diversity, distribution, and secondary metabolites is limited. Marine actinomycetes represent an untapped source of novel bioactive compounds. In this study, we investigated shrimp shell as substrates for model production bioactive metabolites from actinomycetes under solid state fermentation (SSF) conditions. A total of fifteen actinomycetes were isolated from six sponges and one tunicate. The isolated actinomycetes were grown on solid shrimp shells. Cultures of actinomycetes were extracted with ethyl acetate (EtOAc) and extracts were bioassayed for activity against <i>Staphylococcus aureus</i>. One isolate 18A13O1 from the sponge, <i>Rhabdastrella globostellata</i>, exhibited antibacterial activity on primary screening compared to the other samples and was chosen for further study. Visualization using SEM showed aerial and substrate mycelia. Through phylogenetic analysis, it was confirmed that isolate 18A13O1 is a <i>Pseudonocardia carboxydivorans</i>. Purification of an EtOAc extract yielded A13B2, which showed a minimum inhibition concentration against <i>S. aureus</i> at 15.6 μg/mL. It can be concluded that this basic information is very important for further studies related to the development of the production of bioactive secondary metabolites through the solid state fermentation process.https://www.mdpi.com/2311-5637/7/4/247actinomycetesantibacteria<i>Staphylococcus aureus</i>solid state cultivation |
spellingShingle | Andi Setiawan Widyastuti Widyastuti Arik Irawan Oklis Syahrin Wijaya Aspita Laila Wawan Abdullah Setiawan Ni Luh Gede Ratna Juliasih Kenichi Nonaka Masayoshi Arai John Hendri Solid State Fermentation of Shrimp Shell Waste Using <i>Pseudonocardia carboxydivorans</i> 18A13O1 to Produce Bioactive Metabolites Fermentation actinomycetes antibacteria <i>Staphylococcus aureus</i> solid state cultivation |
title | Solid State Fermentation of Shrimp Shell Waste Using <i>Pseudonocardia carboxydivorans</i> 18A13O1 to Produce Bioactive Metabolites |
title_full | Solid State Fermentation of Shrimp Shell Waste Using <i>Pseudonocardia carboxydivorans</i> 18A13O1 to Produce Bioactive Metabolites |
title_fullStr | Solid State Fermentation of Shrimp Shell Waste Using <i>Pseudonocardia carboxydivorans</i> 18A13O1 to Produce Bioactive Metabolites |
title_full_unstemmed | Solid State Fermentation of Shrimp Shell Waste Using <i>Pseudonocardia carboxydivorans</i> 18A13O1 to Produce Bioactive Metabolites |
title_short | Solid State Fermentation of Shrimp Shell Waste Using <i>Pseudonocardia carboxydivorans</i> 18A13O1 to Produce Bioactive Metabolites |
title_sort | solid state fermentation of shrimp shell waste using i pseudonocardia carboxydivorans i 18a13o1 to produce bioactive metabolites |
topic | actinomycetes antibacteria <i>Staphylococcus aureus</i> solid state cultivation |
url | https://www.mdpi.com/2311-5637/7/4/247 |
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