Correlations between SmCPS1 promoter polymorphism and tanshinone contents in Salvia miltiorrhiza
Copalyl diphosphate synthase 1 (SmCPS1) is the first committed enzyme in tanshinone biosynthesis. The promoter region plays an important role in the transcriptional regulation of genes. Mutations in the promoter region may affect gene expression, resulting in changes in the amount of metabolites. In...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2018-12-01
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| Series: | Czech Journal of Genetics and Plant Breeding |
| Subjects: | |
| Online Access: | https://cjgpb.agriculturejournals.cz/artkey/cjg-201804-0006_correlations-between-smcps1-promoter-polymorphism-and-tanshinone-contents-in-salvia-miltiorrhiza.php |
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| author | Ying SUN Xin CHEN Xiao-Yan GAN Zhu-Yun YAN Di-Xiu MU Qing-Rong WANG |
| author_facet | Ying SUN Xin CHEN Xiao-Yan GAN Zhu-Yun YAN Di-Xiu MU Qing-Rong WANG |
| author_sort | Ying SUN |
| collection | DOAJ |
| description | Copalyl diphosphate synthase 1 (SmCPS1) is the first committed enzyme in tanshinone biosynthesis. The promoter region plays an important role in the transcriptional regulation of genes. Mutations in the promoter region may affect gene expression, resulting in changes in the amount of metabolites. In this study, we investigated the SmCPS1 gene promoter region together with the 388 bp downstream from the translation start site and the content of tanshinones of 12 different genotypes of Salvia miltiorrhiza. The cis-elements of SmCPS1 promoter were predicted and analysed by the Plant Transcriptional Regulatory Map database. We found (1) a different correlation between the polymorphism in the promoter region and the contents of tanshinones; (2) functional polymorphic loci - four tandem repeat variations, three indels and five single nucleotide polymorphisms (SNPs) in five cis-elements, three SNPs in exons and two SNPs in introns; (3) the correlation coefficient was higher when only functional (informative) polymorphic loci were considered. These findings have laid the foundation for further exploring the interspecific variation of S. miltiorrhiza and its relationship with the contents of tanshinones. |
| first_indexed | 2024-04-10T08:29:05Z |
| format | Article |
| id | doaj.art-144a15c3991b48e48999725fdda241d6 |
| institution | Directory Open Access Journal |
| issn | 1212-1975 1805-9325 |
| language | English |
| last_indexed | 2024-04-10T08:29:05Z |
| publishDate | 2018-12-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Czech Journal of Genetics and Plant Breeding |
| spelling | doaj.art-144a15c3991b48e48999725fdda241d62023-02-23T03:30:42ZengCzech Academy of Agricultural SciencesCzech Journal of Genetics and Plant Breeding1212-19751805-93252018-12-0154417718210.17221/97/2017-CJGPBcjg-201804-0006Correlations between SmCPS1 promoter polymorphism and tanshinone contents in Salvia miltiorrhizaYing SUN0Xin CHENXiao-Yan GAN1Zhu-Yun YAN2Di-Xiu MU3Qing-Rong WANG4Chengdu University of Traditional Chinese Medicine, Chengdu, P.R. ChinaChengdu University of Traditional Chinese Medicine, Chengdu, P.R. ChinaChengdu University of Traditional Chinese Medicine, Chengdu, P.R. ChinaChengdu University of Traditional Chinese Medicine, Chengdu, P.R. ChinaChengdu University of Traditional Chinese Medicine, Chengdu, P.R. ChinaCopalyl diphosphate synthase 1 (SmCPS1) is the first committed enzyme in tanshinone biosynthesis. The promoter region plays an important role in the transcriptional regulation of genes. Mutations in the promoter region may affect gene expression, resulting in changes in the amount of metabolites. In this study, we investigated the SmCPS1 gene promoter region together with the 388 bp downstream from the translation start site and the content of tanshinones of 12 different genotypes of Salvia miltiorrhiza. The cis-elements of SmCPS1 promoter were predicted and analysed by the Plant Transcriptional Regulatory Map database. We found (1) a different correlation between the polymorphism in the promoter region and the contents of tanshinones; (2) functional polymorphic loci - four tandem repeat variations, three indels and five single nucleotide polymorphisms (SNPs) in five cis-elements, three SNPs in exons and two SNPs in introns; (3) the correlation coefficient was higher when only functional (informative) polymorphic loci were considered. These findings have laid the foundation for further exploring the interspecific variation of S. miltiorrhiza and its relationship with the contents of tanshinones.https://cjgpb.agriculturejournals.cz/artkey/cjg-201804-0006_correlations-between-smcps1-promoter-polymorphism-and-tanshinone-contents-in-salvia-miltiorrhiza.phpcis-elementscopalyl diphosphate synthase 1danshenpolymorphism |
| spellingShingle | Ying SUN Xin CHEN Xiao-Yan GAN Zhu-Yun YAN Di-Xiu MU Qing-Rong WANG Correlations between SmCPS1 promoter polymorphism and tanshinone contents in Salvia miltiorrhiza Czech Journal of Genetics and Plant Breeding cis-elements copalyl diphosphate synthase 1 danshen polymorphism |
| title | Correlations between SmCPS1 promoter polymorphism and tanshinone contents in Salvia miltiorrhiza |
| title_full | Correlations between SmCPS1 promoter polymorphism and tanshinone contents in Salvia miltiorrhiza |
| title_fullStr | Correlations between SmCPS1 promoter polymorphism and tanshinone contents in Salvia miltiorrhiza |
| title_full_unstemmed | Correlations between SmCPS1 promoter polymorphism and tanshinone contents in Salvia miltiorrhiza |
| title_short | Correlations between SmCPS1 promoter polymorphism and tanshinone contents in Salvia miltiorrhiza |
| title_sort | correlations between smcps1 promoter polymorphism and tanshinone contents in salvia miltiorrhiza |
| topic | cis-elements copalyl diphosphate synthase 1 danshen polymorphism |
| url | https://cjgpb.agriculturejournals.cz/artkey/cjg-201804-0006_correlations-between-smcps1-promoter-polymorphism-and-tanshinone-contents-in-salvia-miltiorrhiza.php |
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