Upregulated lncRNA‐NEF predicts recurrence and poor treatment outcomes of ankylosing spondylitis
Abstract Introduction Osteoporosis is related to lncRNA‐neighboring enhancer of FOXA2 (NEF) and inversely correlated to ankylosing spondylitis (AS), implying that lncRNA‐NEF might also relate to AS. Thus, the study was carried out to investigate the involvement of lncRNA‐NEF in AS. Methods The study...
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| Format: | Article |
| Language: | English |
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Wiley
2022-08-01
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| Series: | Immunity, Inflammation and Disease |
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| Online Access: | https://doi.org/10.1002/iid3.627 |
| _version_ | 1811312679606812672 |
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| author | Dapeng Han Guilin Ouyang Peijun Pan Yuan Yuan |
| author_facet | Dapeng Han Guilin Ouyang Peijun Pan Yuan Yuan |
| author_sort | Dapeng Han |
| collection | DOAJ |
| description | Abstract Introduction Osteoporosis is related to lncRNA‐neighboring enhancer of FOXA2 (NEF) and inversely correlated to ankylosing spondylitis (AS), implying that lncRNA‐NEF might also relate to AS. Thus, the study was carried out to investigate the involvement of lncRNA‐NEF in AS. Methods The study included 60 AS patients and 60 healthy controls. LncRNA‐NEF expression in synovial fluid samples was analyzed by reverse transcription quantitative real‐time polymerase chain reaction. Disease activity of the 60 AS patients was determined using the Ankylosing Spondylitis Disease Activity Score (ASDAS) 1–4 and Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). Western blot was carried out to investigate the effects of lncRNA‐NEF on inflammatory factors in human fibroblast‐like synovial (HFLS) cells. A 3‐year follow‐up was performed to analyze the role of lncRNA‐NEF in the prediction of the recurrence of AS. Results Our study observed that lncRNA‐NEF expression was upregulated in synovial fluid of AS patients and significantly correlated with the ASDAS 1–4, BASDAI, erythrocyte sedimentation rate (ESR), and C‐reactive protein level (p < .05). Treatment with nonsteroidal anti‐inflammatory drugs significantly downregulated lncRNA‐NEF expression (p < .01). A 3‐year follow‐up showed that patients with high lncRNA‐NEF levels had a high recurrence rate (hazard ratio = 2.266). In addition, lncRNA‐NEF was found to regulate the expression of inflammatory factors in HFLS cells. Conclusions Therefore, lncRNA‐NEF upregulation can predict recurrence and poor treatment outcomes of AS and has a great potential to serve as a predictive biomarker factor for the recurrent AS. |
| first_indexed | 2024-04-13T10:41:05Z |
| format | Article |
| id | doaj.art-144c47ed05184f11a6b88b0d51458736 |
| institution | Directory Open Access Journal |
| issn | 2050-4527 |
| language | English |
| last_indexed | 2024-04-13T10:41:05Z |
| publishDate | 2022-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Immunity, Inflammation and Disease |
| spelling | doaj.art-144c47ed05184f11a6b88b0d514587362022-12-22T02:49:55ZengWileyImmunity, Inflammation and Disease2050-45272022-08-01108n/an/a10.1002/iid3.627Upregulated lncRNA‐NEF predicts recurrence and poor treatment outcomes of ankylosing spondylitisDapeng Han0Guilin Ouyang1Peijun Pan2Yuan Yuan3Department of Orthopaedics Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine Shanghai ChinaDepartment of Orthopaedics Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine Shanghai ChinaDepartment of Orthopaedics Shanghai Guanghua Hospital of Integrated Traditional Chinese and Western Medicine Shanghai ChinaDepartment of the Health and Nursing Shanghai Nanhu Vocational and Technical College Shanghai ChinaAbstract Introduction Osteoporosis is related to lncRNA‐neighboring enhancer of FOXA2 (NEF) and inversely correlated to ankylosing spondylitis (AS), implying that lncRNA‐NEF might also relate to AS. Thus, the study was carried out to investigate the involvement of lncRNA‐NEF in AS. Methods The study included 60 AS patients and 60 healthy controls. LncRNA‐NEF expression in synovial fluid samples was analyzed by reverse transcription quantitative real‐time polymerase chain reaction. Disease activity of the 60 AS patients was determined using the Ankylosing Spondylitis Disease Activity Score (ASDAS) 1–4 and Bath Ankylosing Spondylitis Disease Activity Index (BASDAI). Western blot was carried out to investigate the effects of lncRNA‐NEF on inflammatory factors in human fibroblast‐like synovial (HFLS) cells. A 3‐year follow‐up was performed to analyze the role of lncRNA‐NEF in the prediction of the recurrence of AS. Results Our study observed that lncRNA‐NEF expression was upregulated in synovial fluid of AS patients and significantly correlated with the ASDAS 1–4, BASDAI, erythrocyte sedimentation rate (ESR), and C‐reactive protein level (p < .05). Treatment with nonsteroidal anti‐inflammatory drugs significantly downregulated lncRNA‐NEF expression (p < .01). A 3‐year follow‐up showed that patients with high lncRNA‐NEF levels had a high recurrence rate (hazard ratio = 2.266). In addition, lncRNA‐NEF was found to regulate the expression of inflammatory factors in HFLS cells. Conclusions Therefore, lncRNA‐NEF upregulation can predict recurrence and poor treatment outcomes of AS and has a great potential to serve as a predictive biomarker factor for the recurrent AS.https://doi.org/10.1002/iid3.627ankylosing spondylitislncRNA‐NEFrecurrencesynovial fluid |
| spellingShingle | Dapeng Han Guilin Ouyang Peijun Pan Yuan Yuan Upregulated lncRNA‐NEF predicts recurrence and poor treatment outcomes of ankylosing spondylitis Immunity, Inflammation and Disease ankylosing spondylitis lncRNA‐NEF recurrence synovial fluid |
| title | Upregulated lncRNA‐NEF predicts recurrence and poor treatment outcomes of ankylosing spondylitis |
| title_full | Upregulated lncRNA‐NEF predicts recurrence and poor treatment outcomes of ankylosing spondylitis |
| title_fullStr | Upregulated lncRNA‐NEF predicts recurrence and poor treatment outcomes of ankylosing spondylitis |
| title_full_unstemmed | Upregulated lncRNA‐NEF predicts recurrence and poor treatment outcomes of ankylosing spondylitis |
| title_short | Upregulated lncRNA‐NEF predicts recurrence and poor treatment outcomes of ankylosing spondylitis |
| title_sort | upregulated lncrna nef predicts recurrence and poor treatment outcomes of ankylosing spondylitis |
| topic | ankylosing spondylitis lncRNA‐NEF recurrence synovial fluid |
| url | https://doi.org/10.1002/iid3.627 |
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